Cannabinoid Receptor 1 (CNR1) antibody

Antigen: Cannabinoid Receptor 1 (CNR1)

Synonyms: CB1, CNR, CB-R, CB1A, CB1R, CANN6, CB1K5, SKR6R, CNR1, LOC540382

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN152711

Additional Information

Alternative name: Cannabinoid receptor 1

Gene ID: 1268, 25248

Immunogen: Fusion protein, corresponding to amino acids 1-99 of Human Cannabinoid Receptor I.

Cross-Reactivity: Human, Rat (Rattus)

Description: Cannabinoids exert their well known physiological effects through two G protein coupledreceptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors havebeen shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated proteinkinase, MAPK. CB1 receptors also modulate ion channels through direct G-proteininteractions. Delta 9-tetrahydrocannibinol and related ligands likely exert theirpsychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2is thought to function primarily in the immune system although it has been suggested tobe present in the central nervous system, including the retina. Alternate Names: anti-CANN6 antibody, anti-CB R antibody, anti-CB1 antibody, anti-CB1A antibody,anti-CB1K5 antibody, anti-Central cannabinoid receptor isoform a antibody, anti-Centralcannabinoid receptor isoform b antibody, anti-CNR antibody.

Specificity: Detects CB1 from human and rat tissues as well as transfected rat CB1. Species Reactivity: Cross-reacts with Human and Rat.Not yet tested in other species. Localization: Integral membrane protein.

Application Details

Application Notes: Western Blot: use at a dilution of 1:250. Immunocytochemistry: use at a dilution of1:1000. Optimal dilutions / concentrations should be determined by the end user. ByWestern blot, this antibody detects an ~60 kDa protein representing CB1 from rat brainhomogenate. Immunocytochemical staining of CB1 in AtT20 cells transfected with therat CB1 gene yields a pattern consistent with plasma membrane staining.

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: PBS, 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide. Preservative: 0.05% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Receptors

Restrictions: For Research Use only

Images

Figure 1 shows immunlocalization of rat CB1 transfected into AtT20 cells using ABIN152711.

Publications

Mulhern, Madson, Danford et al.: "The unfolded protein response in lens epithelial cells from galactosemic rat lenses." in: Investigative ophthalmology & visual science, Vol. 47, Issue 9, pp. 3951-9, 2006 (PubMed).