Proteasome (Prosome, Macropain) Subunit, alpha Type, 1 (PSMA1) antibody

Antigen: Proteasome (Prosome, Macropain) Subunit, alpha Type, 1 (PSMA1)

Synonyms: NU, HC2, PROS30, MGC1667, MGC14542, MGC14575, MGC14751, MGC21459, MGC22853, MGC23915, C2, alpha-type, CC2, PSMA1, MGC90008, MGC127224, MGC115143, DKFZp468K0822

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine)

Application: Western Blotting (WB)

Catalog no.: ABIN152777

Additional Information

Alternative name: PSMA1

Immunogen: Synthetic Peptide: CP(249)ADEPAEKADEPMEH(263).Immunizing peptide correspondsto amino acid residues 249-263 from the C-terminus of human proteasome 20S C2subunit. This sequence is 93% conserved in the rat and chicken protein and 87%conserved in the mouse protein.

Cross-Reactivity: Dog (Canine), Hamster, Human, Mouse (Murine), Rat (Rattus)

Specificity: Detects proteasome 20S C2 subunit. Species Reactivity: Cross-reacts with Human, Mouse, Rat, Dog and Hamster. Expected to cross-react withChicken due to sequence homology.

Application Details

Application Notes: Western Blot: use at 1 ug/ml. By Western blot, this antibody detects a 29 kDa proteinrepresenting proteasome 20S C2 subunit from rat brain extract.

Purification: Aff - Purified

Buffer: PBS with 1 mg/ml BSA

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Figure 1 shows a Western blot of 20S C2 subunit in CHO cell extract using ABIN152777.

Publications

Schwartz, Ciechanover: "The ubiquitin-proteasome pathway and pathogenesis of human diseases." in: Annual review of medicine, Vol. 50, pp. 57-74, 1999 (PubMed).

Voges, Zwickl, Baumeister: "The 26S proteasome: a molecular machine designed for controlled proteolysis." in: Annual review of biochemistry, Vol. 68, pp. 1015-68, 2000 (PubMed).

Vishnyakova, Kurlander, Bocharov et al.: "CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 45, pp. 16888-93, 2006 (PubMed).

Laporte, Salin, Daignan-Fornier et al.: "Reversible cytoplasmic localization of the proteasome in quiescent yeast cells." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 737-45, 2008 (PubMed).