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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
phosphodiesterase 4A, CAMP-Specific (PDE4A) antibody
|Synonyms||PDE4, DPDE2, PDE46, Dpde2, D9Ertd60e, PHOSA, PDE4A, FLJ12949, pde4, dpde2, pde46, Pde4a|
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (1 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Gene ID||5141, 18577, 25638|
|Immunogen||Synthetic peptide corresponding to the C-terminal region of PDE4A (common to allPDE4A proteins).|
|Cross-Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus)|
|Description||Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family areimportant in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR)stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by thestimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are alsoimportant molecular targets for a variety of therapeutic agents like antidepressants,anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A,B, C and D), each exhibiting multiple isozymes due to alternate splicing that leads to alarger number of distinct PDE4 variants. Members of the PDE4 family areregulated/activated by phosphorylation/dephosphorylation by cAMP-dependent proteinkinase A and phosphatases. Protein-protein interactions and cellular trafficking ofPDE4A enzymes play an important role in cAMP compartmentalization andcAMP-dependent signaling. In brain members of the PDE4A, B and D family areassociated with GPCRs (adrenergic and dopaminergic) signaling. Alternate Names: Anti-cAMP specific 3 5 cyclic phosphodiesterase 4A antibody, anti- cAMP specific phosphodiesterase antibody, anti- Cyclic AMP phosphodiesterasePDE4A11 antibody, anti- Cyclic AMP specific phosphodiesterase HSPDE4A10 antibody,anti- DPDE 2 antibody, anti- dunce like phosphodiesterase E2 antibody, anti- PDE 4antibody.|
|Specificity||This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), A5 (109kDa), A8 (106 kDa), Ax (102 kDa) and a 76 kDa testis specific A variant. Species Reactivity: Cross-reacts with Human, Mouse, Rat and Rabbit.Not yet tested in other species. Localization: Cell Membrane.|
|Application Notes||Suggested working dilutions: immunoprecipitation 1:250,Western Blot 1:1000,Immunocytochemistry|
|Purification||Aff - Purified|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
|anti-phosphodiesterase 4A, CAMP-Specific (PDE4A) antibody|
Farooqui, Brock, Hamdi et al.: "Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum." in: Journal of neurochemistry, Vol. 57, Issue 4, pp. 1363-9, 1991 (PubMed).
Beavo: "Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms." in: Physiological reviews, Vol. 75, Issue 4, pp. 725-48, 1995 (PubMed).
Yarwood, Steele, Scotland et al.: "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14909-17, 1999 (PubMed).
Zhang, Crissman, Dorairaj et al.: "Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 23, Issue 2, pp. 198-204, 2000 (PubMed).
Hagendorf, Fluegge, Engelhardt et al.: "Homeostatic control of sensory output in basal vomeronasal neurons: activity-dependent expression of ether-à-go-go-related gene potassium channels." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 1, pp. 206-21, 2009 (PubMed).
|Hosts||Rabbit (44), Mouse (11)|
|Reactivities||Human (39), Mouse (Murine) (22), Rat (Rattus) (22), Chicken (3), Cat (Feline) (1), Cow (Bovine) (1), Dog (Canine) (1), Monkey (1)|
|Applications||Immunofluorescence (IF) (30), Western Blotting (WB) (18), Immunohistochemistry (IHC) (17), Flow Cytometry (FACS) (12), ELISA (9), Immunoprecipitation (IP) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (5), FITC (5), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Transcript Variant 4 (11), C-Term (4), Isoform specific (2), Phosphospecific (1), phosphorylated (1)|