phosphodiesterase 4A, CAMP-Specific (PDE4A) antibody

Antigen: phosphodiesterase 4A, CAMP-Specific (PDE4A)

Synonyms: PDE4, DPDE2, PDE46, Dpde2, D9Ertd60e, PHOSA, PDE4A, FLJ12949, pde4, dpde2, pde46, Pde4a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine)

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN152828

Additional Information

Alternative name: PDE4A

Gene ID: 5141, 18577, 25638

Immunogen: Synthetic peptide corresponding to the C-terminal region of PDE4A (common to allPDE4A proteins).

Cross-Reactivity: Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Description: Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family areimportant in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR)stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by thestimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are alsoimportant molecular targets for a variety of therapeutic agents like antidepressants,anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A,B, C and D), each exhibiting multiple isozymes due to alternate splicing that leads to alarger number of distinct PDE4 variants. Members of the PDE4 family areregulated/activated by phosphorylation/dephosphorylation by cAMP-dependent proteinkinase A and phosphatases. Protein-protein interactions and cellular trafficking ofPDE4A enzymes play an important role in cAMP compartmentalization andcAMP-dependent signaling. In brain members of the PDE4A, B and D family areassociated with GPCRs (adrenergic and dopaminergic) signaling. Alternate Names: Anti-cAMP specific 3 5 cyclic phosphodiesterase 4A antibody, anti- cAMP specific phosphodiesterase antibody, anti- Cyclic AMP phosphodiesterasePDE4A11 antibody, anti- Cyclic AMP specific phosphodiesterase HSPDE4A10 antibody,anti- DPDE 2 antibody, anti- dunce like phosphodiesterase E2 antibody, anti- PDE 4antibody.

Specificity: This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), A5 (109kDa), A8 (106 kDa), Ax (102 kDa) and a 76 kDa testis specific A variant. Species Reactivity: Cross-reacts with Human, Mouse, Rat and Rabbit.Not yet tested in other species. Localization: Cell Membrane.

Application Details

Application Notes: Suggested working dilutions: immunoprecipitation 1:250,Western Blot 1:1000,Immunocytochemistry

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: 02% Thimerosal/Merthiolate.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Signaling

Restrictions: For Research Use only

Images

anti-phosphodiesterase 4A, CAMP-Specific (PDE4A) antibody

Publications

Farooqui, Brock, Hamdi et al.: "Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum." in: Journal of neurochemistry, Vol. 57, Issue 4, pp. 1363-9, 1991 (PubMed).

Beavo: "Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms." in: Physiological reviews, Vol. 75, Issue 4, pp. 725-48, 1995 (PubMed).

Yarwood, Steele, Scotland et al.: "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14909-17, 1999 (PubMed).

Zhang, Crissman, Dorairaj et al.: "Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 23, Issue 2, pp. 198-204, 2000 (PubMed).

Hagendorf, Fluegge, Engelhardt et al.: "Homeostatic control of sensory output in basal vomeronasal neurons: activity-dependent expression of ether-à-go-go-related gene potassium channels." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 1, pp. 206-21, 2009 (PubMed).