Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37) antibody

Antigen: Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37)

Synonyms: P50CDC37, p50, p50Cdc37

Clonality: Monoclonal (C1)

Host: Mouse

Reactivity: Hamster

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN152873

Additional Information

Alternative name: CDC37

Gene ID: 11140, 12539, 114562

Immunogen: Purified mouse Cdc 37 fusion protein.

Cross-Reactivity: Hamster, Human, Mouse (Murine), Rat (Rattus)

Format: Ascites

Isotype: IgG2b

Clone: C1

Description: In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell divisioncycle through its action on cyclin-dependent kinases (CDK',s). Cdc37 has alsobeen associated with signaling pathways and kinases unrelated to cell cycle control,including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosinekinase. Interestingly, HSP90 has also been shown to be associated with many of thesame cellular targets as Cdc37.It has now been shown that Cdc37 is the same protein previously described as p50which has been found to be associated with many of the same proteins as HSP90.Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn,Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown tobe a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessaryfor the binding of HSP90 to Cdk4. Alternate Names: anti-S cerevisiae hypothetical protein CDC37 antibody, anti-CDC 37 antibody,anti-CDC37 antibody, anti-CDC37 cell division cycle 37 homolog antibody, anti-Cdc37homolog antibody, anti-CDC37 protein antibody, anti-Cell division cycle 37 homologantibody, anti-Hsp90 chaperone protein kinase targeting subunit antibody, anti-Hsp90chaperone protein kinase targeting subunit p50Cdc37 antibody, anti-Hsp90 cochaperone Cdc37 antibody, anti-p50 antibody, anti-p50Cdc37 antibody.

Specificity: Species Reactivity: Cross-reacts with Human, Rat, Mouse and Hamster.Not yet tested in other species. Localization: Cytoplasmic and Nuclear.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:5,000,Immunocytochemistry 1:200

Purification: Ascites

Buffer: Preservative: 0.05% sodium azide

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Cell Cycle, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Perdew, Wiegand, Vanden Heuvel et al.: "A 50 kilodalton protein associated with raf and pp60(v-src) protein kinases is a mammalian homolog of the cell cycle control protein cdc37." in: Biochemistry, Vol. 36, Issue 12, pp. 3600-7, 1997 (PubMed).

Lamphere, Fiore, Xu et al.: "Interaction between Cdc37 and Cdk4 in human cells." in: Oncogene, Vol. 14, Issue 16, pp. 1999-2004, 1997 (PubMed).

Kimura, Rutherford, Miyata et al.: "Cdc37 is a molecular chaperone with specific functions in signal transduction." in: Genes & development, Vol. 11, Issue 14, pp. 1775-85, 1997 (PubMed).