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Scavenger Receptor Class B, Member 1 (SCARB1) (C-Term), (AA 450-509) antibody

Details for Product No. ABIN152880
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Antigen
Synonyms cb1015, SCARB1, Scarb1, CD36L1, CLA-1, CLA1, HDLQTL6, SR-BI, SRB1, AI120173, CD36, Cd36l1, Cla-1, Cla1, D5Ertd460e, Hdlq1, Hlb398, SR-B1, SRBI, Srb1, mSR-BI
Epitope
C-Term, AA 450-509
(18), (12), (7), (5), (3)
Reactivity
Human, Mouse (Murine), Rat (Rattus), Mustelid
(48), (48), (41), (17), (17), (17), (4), (2)
Host
Rabbit
(60), (8), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Blocking Reagent (BR), Neutralization (Neut), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(51), (20), (18), (18), (14), (13), (11), (10), (8), (6), (3), (1)
Pubmed 13 references available
Quantity 0.1 mL
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Catalog No. ABIN152880
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Immunogen A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509).
Cross-Reactivity (Details) Bovine reactivity reported in scientific literature (PMID: 24196350)
Purification affinity purified
Alternative Name SCARB1
Background High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and theirplasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BIbinds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDLwith high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues,and mediates selective cholesterol uptake by a distinct mechanism.In mice, it seems that SR-BI plays a key role in determining the levels of plasmalipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland. Alternate Names: anti-SCARB1 antibody, anti-SR-B1 antibody, anti-SR-BI antibody, anti-ScavengerReceptor-BI antibody, anti-Scavenger Receptor-B1 antibody, anti-HCLA 1 antibody,anti-SRBI antibody, anti-SR BI antibody, anti-SRB1 antibody, anti-CD36 and LIMPIIanalogous 1 antibody, anti-CD36 antigen antibody, anti-CD36L1 antibody, anti-CLA1antibody, anti-CLA 1 antibody, anti-Collagen type I receptor, anti-thrombospondinreceptor-like 1 antibody, anti-Scavenger Receptor Class B Type 1 antibody,anti-scavenger receptor class B type 1 antibody, anti-scavenger receptor class B type IIIantibody, anti-scavenger receptor class B, member 1 antibody, anti-MGC138242antibody. Related Diseases: Cancer, Coronary Artery Disease, Atherosclerosis
Gene Symbol: SCARB1
Gene ID 949, 20778, 25073
UniProt Q61009
Application Notes This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.Blocking/Neutralizing was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566)
Recommended dilutions: Blocking/Neutralizing, Flow Cytometry 1:400, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry 2.5-5 µg/mL, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 2.5-5 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1:1000-1:5000
Protocol Western Blot protocol specific for SR-BI Antibody Procedure Guide for NB400-101 Polyclonal anti-SR-B1Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences.*NOTE: HL-60 whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
anti-Scavenger Receptor Class B, Member 1 (SCARB1) (C-Term), (AA 450-509) antibody Detection of SR-BI in mouse liver lysate (20 ug) using ABIN152880. ECL detection 5 seconds.
General Frolov, Petrescu, Atshaves et al.: "High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. A single- and multiphoton fluorescence approach." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12769-80, 2000 (PubMed).

Wang, Silver, Costet et al.: "Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 33053-8, 2000 (PubMed).

Husemann, Loike, Kodama et al.: "Scavenger receptor class B type I (SR-BI) mediates adhesion of neonatal murine microglia to fibrillar beta-amyloid." in: Journal of neuroimmunology, Vol. 114, Issue 1-2, pp. 142-50, 2001 (PubMed).

Han, Nicholson, Zhou et al.: "Oxidized low density lipoprotein decreases macrophage expression of scavenger receptor B-I." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16567-72, 2001 (PubMed).

Ohgami, Nagai, Miyazaki et al.: "Scavenger receptor class B type I-mediated reverse cholesterol transport is inhibited by advanced glycation end products." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13348-55, 2001 (PubMed).

Silver, Wang, Xiao et al.: "High density lipoprotein (HDL) particle uptake mediated by scavenger receptor class B type 1 results in selective sorting of HDL cholesterol from protein and polarized cholesterol secretion." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25287-93, 2001 (PubMed).

Cai, Kirby, Howles et al.: "Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine." in: Journal of lipid research, Vol. 42, Issue 6, pp. 902-9, 2001 (PubMed).

Tsuruoka, Khovidhunkit, Brown et al.: "Scavenger receptor class B type I is expressed in cultured keratinocytes and epidermis. Regulation in response to changes in cholesterol homeostasis and barrier requirements." in: The Journal of biological chemistry, Vol. 277, Issue 4, pp. 2916-22, 2002 (PubMed).

Huang, Mazzone: "ApoE-dependent sterol efflux from macrophages is modulated by scavenger receptor class B type I expression." in: Journal of lipid research, Vol. 43, Issue 3, pp. 375-82, 2002 (PubMed).

Silver: "A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34042-7, 2002 (PubMed).

Willner, Tow, Buhman et al.: "Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1262-7, 2003 (PubMed).

Basso, Freeman, Knapper et al.: "Role of the hepatic ABCA1 transporter in modulating intrahepatic cholesterol and plasma HDL cholesterol concentrations." in: Journal of lipid research, Vol. 44, Issue 2, pp. 296-302, 2003 (PubMed).

Zhang, Ahmed, McFarlane et al.: "Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways." in: Journal of lipid research, Vol. 48, Issue 2, pp. 405-16, 2007 (PubMed).

Validation Images
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