SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term)

Details for Product anti-SCARB1 Antibody No. ABIN152880, Supplier: Log in to see
Antigen
  • cb1015
  • SCARB1
  • Scarb1
  • CD36L1
  • CLA-1
  • CLA1
  • HDLQTL6
  • SR-BI
  • SRB1
  • AI120173
  • CD36
  • Cd36l1
  • Cla-1
  • Cla1
  • D5Ertd460e
  • Hdlq1
  • Hlb398
  • SR-B1
  • SRBI
  • Srb1
  • mSR-BI
  • scavenger receptor class B, member 1
  • scavenger receptor class B member 1
  • scarb1
  • SCARB1
  • Scarb1
  • CpipJ_CPIJ014332
  • CpipJ_CPIJ019101
Alternatives
anti-Schistosoma japonicum SCARB1 antibody for Blocking Peptide
Epitope
C-Term, AA 450-509
50
27
17
16
15
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Chinese Hamster, Cow (Bovine), Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus), Schistosoma japonicum
103
75
73
37
25
25
25
15
14
13
12
12
2
2
1
Host
Rabbit
81
26
16
Clonality
Polyclonal
Conjugate
This SCARB1 antibody is un-conjugated
6
6
5
5
4
4
4
3
3
3
3
3
2
2
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Simple Western (SimWes), Western Blotting (WB)
104
55
44
43
41
41
32
30
12
5
3
2
1
1
1
1
Options
Supplier
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Immunogen A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Purification Immunogen affinity purified
Alternative Name SR-BI (SCARB1 Antibody Abstract)
Background Gene Symbol: SCARB1
Molecular Weight Theoretical MW: 82 kDa
Gene ID 949
UniProt Q8WTV0, Q61009, P97943
Pathways Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Application Notes Western Blot 1:1000-1:5000, Simple Western 1:100, Flow Cytometry 1:400, Immunohistochemistry 2.5-5 μg/mL, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 2.5-5 μg/mL, Immunohistochemistry-Frozen, Blocking/NeutralizingThis SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.Blocking/Neutralizing was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566) In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for SR-BI Antibody Procedure Guide for NB400-101 Polyclonal anti-SR-B1Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences.*NOTE: HL-60 whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152880) Detection of SR-BI in mouse liver lysate (20 ug) using ABIN152880. ECL detection 5 s...
Simple Western (SimWes) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152880) Simple Western: SR-BI Antibody [ABIN152880] - Simple Western lane view shows a specif...
Flow Cytometry (FACS) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152880) Flow Cytometry: SR-BI Antibody [ABIN152880] - SR-BI antibody was tested at 1:400 in H...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152880) Immunohistochemistry-Paraffin: SR-BI Antibody [ABIN152880] - Scavenger receptor class...
Immunofluorescence (IF) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152880) Immunocytochemistry/Immunofluorescence: SR-BI Antibody [ABIN152880] - SRB1 antibody w...
Product cited in: Tsugita, Morimoto, Tashiro, Kinoshita, Nakayama: "SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation." in: Cell reports, Vol. 18, Issue 5, pp. 1298-1311, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Bartelt, John, Schaltenberg, Berbée, Worthmann, Cherradi, Schlein, Piepenburg, Boon, Rinninger, Heine, Toedter, Niemeier, Nilsson, Fischer, Wijers, van Marken Lichtenbelt, Scheja, Rensen, Heeren: "Thermogenic adipocytes promote HDL turnover and reverse cholesterol transport." in: Nature communications, Vol. 8, pp. 15010, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Pasterk, Lemesch, Leber, Trieb, Curcic, Stadlbauer, Schuligoi, Schicho, Heinemann, Marsche: "Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression." in: Scientific reports, Vol. 6, pp. 22104, 2016 (PubMed). (Sample species: Human). Further details: Blocking Peptide,Neutralization

Conlon, Thomas, Fedotova, Hernandez-Ono, Di Paolo, Chan, Ruggles, Gibeley, Liu, Ginsberg: "Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis." in: The Journal of clinical investigation, Vol. 126, Issue 10, pp. 3852-3867, 2016 (PubMed).

Vaisman, Vishnyakova, Freeman, Amar, Demosky, Liu, Stonik, Sampson, Pryor, Bocharov, Eggerman, Patterson, Remaley: "Endothelial Expression of Scavenger Receptor Class B, Type I Protects against Development of Atherosclerosis in Mice." in: BioMed research international, Vol. 2015, pp. 607120, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kartz, Holme, Nicholson, Sahoo: "SR-BI/CD36 chimeric receptors define extracellular subdomains of SR-BI critical for cholesterol transport." in: Biochemistry, Vol. 53, Issue 39, pp. 6173-82, 2014 (PubMed).

Okumura-Noji, Miura, Lu, Asai, Ohta, Brindley, Yokoyama: "CD36-related protein in Schistosoma japonicum: candidate mediator of selective cholesteryl ester uptake from high-density lipoprotein for egg maturation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 3, pp. 1236-44, 2013 (PubMed). Further details: Western Blotting

Chakraborty, Lou, Huan, Kuo, Park, Cao, Jiang: "Myeloid cell-specific serine palmitoyltransferase subunit 2 haploinsufficiency reduces murine atherosclerosis." in: The Journal of clinical investigation, Vol. 123, Issue 4, pp. 1784-97, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Flow Cytometry

Béaslas, Metso, Nissilä, Laurila, Kaiharju, Batchu, Kaipiainen, Mäyränpää, Yan, Gylling, Jauhiainen, Olkkonen: "Osbpl8 deficiency in mouse causes an elevation of high-density lipoproteins and gender-specific alterations of lipid metabolism." in: PLoS ONE, Vol. 8, Issue 3, pp. e58856, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Freeman: "Western blots." in: Methods in molecular biology (Clifton, N.J.), Vol. 1027, pp. 369-85, 2013 (PubMed). (Sample species: Human).

Nicod, Parker: "Vitamin E secretion by Caco-2 monolayers to APOA1, but not to HDL, is vitamer selective." in: The Journal of nutrition, Vol. 143, Issue 10, pp. 1565-72, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Mao, Hou, Talbott, Cushman, Cupp, Davis: "ATF3 expression in the corpus luteum: possible role in luteal regression." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 12, pp. 2066-79, 2013 (PubMed). (Sample species: Cow (Bovine)). Further details: Western Blotting

Robert, Weber, Frese, Emmert, Schmidt, von Eckardstein, Rohrer, Hoerstrup: "A three-dimensional engineered artery model for in vitro atherosclerosis research." in: PLoS ONE, Vol. 8, Issue 11, pp. e79821, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Kushiyama, Okubo, Sakoda, Kikuchi, Fujishiro, Sato, Kushiyama, Iwashita, Nishimura, Fukushima, Nakatsu, Kamata, Kawazu, Higashi, Kurihara, Asano: "Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 32, Issue 2, pp. 291-8, 2012 (PubMed).

Saha, Graessler, Schwarz, Goettsch, Bornstein, Kopprasch: "Modified high-density lipoprotein modulates aldosterone release through scavenger receptors via extra cellular signal-regulated kinase and Janus kinase-dependent pathways." in: Molecular and cellular biochemistry, Vol. 366, Issue 1-2, pp. 1-10, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Chadwick, Sahoo: "Functional characterization of newly-discovered mutations in human SR-BI." in: PLoS ONE, Vol. 7, Issue 9, pp. e45660, 2012 (PubMed). (Sample species: Primate). Further details: Western Blotting,Flow Cytometry

Papale, Hanson, Sahoo: "Extracellular disulfide bonds support scavenger receptor class B type I-mediated cholesterol transport." in: Biochemistry, Vol. 50, Issue 28, pp. 6245-54, 2011 (PubMed). (Sample species: Primate). Further details: Western Blotting

Dong, Xie, Zhou, Hu, Chen, Duan, Li, Han: "Induction of macrophage scavenger receptor type BI expression by tamoxifen and 4-hydroxytamoxifen." in: Atherosclerosis, Vol. 218, Issue 2, pp. 435-42, 2011 (PubMed).

Truong, Aubin, Falstrault, Brodeur, Brissette: "SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells." in: Cell biochemistry and function, Vol. 28, Issue 6, pp. 480-9, 2010 (PubMed).

Nakaya, Summers, Nicholson, Gotto, Hajjar, Han: "Atherosclerosis in LDLR-knockout mice is inhibited, but not reversed, by the PPARgamma ligand pioglitazone." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2007-14, 2009 (PubMed).