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SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term)

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C-Term, AA 450-509
(23), (15), (9), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(58), (32), (27), (2)
(54), (15), (7)
This SCARB1 antibody is un-conjugated
(4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(59), (26), (24), (20), (9), (7), (6), (6), (5), (2), (2), (1), (1)
Pubmed 23 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509).
Specificity Mouse, rat, mink, hamster, primate, rabbit,the long form of human SR-B
Cross-Reactivity (Details) Reactivity with CHO cells reported in scientific literature (PMID: 12356718).
Purification affinity purified
Alternative Name SCARB1 (SCARB1 Antibody Abstract)
Background High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and theirplasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BIbinds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDLwith high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues,and mediates selective cholesterol uptake by a distinct mechanism.In mice, it seems that SR-BI plays a key role in determining the levels of plasmalipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland. Alternate Names: anti-SCARB1 antibody, anti-SR-B1 antibody, anti-SR-BI antibody, anti-ScavengerReceptor-BI antibody, anti-Scavenger Receptor-B1 antibody, anti-HCLA 1 antibody,anti-SRBI antibody, anti-SR BI antibody, anti-SRB1 antibody, anti-CD36 and LIMPIIanalogous 1 antibody, anti-CD36 antigen antibody, anti-CD36L1 antibody, anti-CLA1antibody, anti-CLA 1 antibody, anti-Collagen type I receptor, anti-thrombospondinreceptor-like 1 antibody, anti-Scavenger Receptor Class B Type 1 antibody,anti-scavenger receptor class B type 1 antibody, anti-scavenger receptor class B type IIIantibody, anti-scavenger receptor class B, member 1 antibody, anti-MGC138242antibody.
Gene Symbol: SCARB1
Gene ID 949, 20778, 25073
UniProt Q61009
Application Notes This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry 2.5-5 µg/mL, Immunohistochemistry-Paraffin 2.5-5 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1:1000-1:5000
Protocol Protocol specific for SR-BI Antibody Procedure Guide for NB 400-104 Polyclonal anti-SR-B1Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:250 dilution of NB400-104 (anti-SR-BI), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts were used as a positive control for this antibody.FACS Procedure
. Resuspend 1x10^6 cells in FACS
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Detection of SR-BI in 25 ug of mouse liver lysate, using ABIN152882 (lotJ) at a 1:1,0...
Flow Cytometry (FACS) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) anti-Scavenger Receptor Class B, Member 1 (SCARB1) (AA 450-509), (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunolocalization of SR-BI in adult mink testis using ABIN152882. SR-BI labeling is ...
Product cited in: Ashraf, Kar, Chen et al.: "Specific oxidized phospholipids inhibit scavenger receptor bi-mediated selective uptake of cholesteryl esters." in: The Journal of biological chemistry, Vol. 283, Issue 16, pp. 10408-14, 2008 (PubMed).

Montoudis, Seidman, Boudreau et al.: "Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake." in: Journal of lipid research, Vol. 49, Issue 5, pp. 961-72, 2008 (PubMed).

Barth, Schnober, Neumann-Haefelin et al.: "Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells." in: Journal of virology, Vol. 82, Issue 7, pp. 3466-79, 2008 (PubMed).

Tietge, Nijstad, Havinga et al.: "Secretory phospholipase A2 increases SR-BI-mediated selective uptake from HDL but not biliary cholesterol secretion." in: Journal of lipid research, Vol. 49, Issue 3, pp. 563-71, 2008 (PubMed).

Eacker, Agrawal, Qian et al.: "Hormonal regulation of testicular steroid and cholesterol homeostasis." in: Molecular endocrinology (Baltimore, Md.), Vol. 22, Issue 3, pp. 623-35, 2008 (PubMed).

Valiyaveettil, Kar, Ashraf et al.: "Oxidized high-density lipoprotein inhibits platelet activation and aggregation via scavenger receptor BI." in: Blood, Vol. 111, Issue 4, pp. 1962-71, 2008 (PubMed).

Yvan-Charvet, Pagler, Wang et al.: "SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL." in: Journal of lipid research, Vol. 49, Issue 1, pp. 107-14, 2007 (PubMed).

During, Harrison: "Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells." in: Journal of lipid research, Vol. 48, Issue 10, pp. 2283-94, 2007 (PubMed).

Qu, Perdomo, Su et al.: "Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice." in: Journal of lipid research, Vol. 48, Issue 7, pp. 1476-87, 2007 (PubMed).

Timmers, Sharma, Opavsky et al.: "E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop." in: Molecular and cellular biology, Vol. 27, Issue 1, pp. 65-78, 2006 (PubMed).

Wadsack, Tabano, Maier et al.: "Intrauterine growth restriction is associated with alterations in placental lipoprotein receptors and maternal lipoprotein composition." in: American journal of physiology. Endocrinology and metabolism, Vol. 292, Issue 2, pp. E476-84, 2007 (PubMed).

Zhong, Gastaminza, Chung et al.: "Persistent hepatitis C virus infection in vitro: coevolution of virus and host." in: Journal of virology, Vol. 80, Issue 22, pp. 11082-93, 2006 (PubMed).

Azhar, Medicherla, Shen et al.: "LDL and cAMP cooperate to regulate the functional expression of the LRP in rat ovarian granulosa cells." in: Journal of lipid research, Vol. 47, Issue 11, pp. 2538-50, 2006 (PubMed).

Akpovi, Yoon, Vitale et al.: "The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2233-47, 2006 (PubMed).

Sané, Sinnett, Delvin et al.: "Localization and role of NPC1L1 in cholesterol absorption in human intestine." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2112-20, 2006 (PubMed).

Thomuas-Moyua, Gianotti, Proenza et al.: "The age-related paraoxonase 1 response is altered by long-term caloric restriction in male and female rats." in: Journal of lipid research, Vol. 47, Issue 9, pp. 2042-8, 2006 (PubMed).

Yesilaltay, Morales, Amigo et al.: "Effects of hepatic expression of the high-density lipoprotein receptor SR-BI on lipoprotein metabolism and female fertility." in: Endocrinology, Vol. 147, Issue 4, pp. 1577-88, 2006 (PubMed).

Harder, Vassiliou, McBride et al.: "Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy." in: Journal of lipid research, Vol. 47, Issue 3, pp. 492-503, 2006 (PubMed).

Johansson, Rudling, Scanlan et al.: "Selective thyroid receptor modulation by GC-1 reduces serum lipids and stimulates steps of reverse cholesterol transport in euthyroid mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10297-302, 2005 (PubMed).

Lemieux, Guelinas, Lalonde et al.: "Hypolipidemic action of the SERM acolbifene is associated with decreased liver MTP and increased SR-BI and LDL receptors." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1285-94, 2005 (PubMed).

Han, Parsons, Zhou et al.: "Functional interplay between the macrophage scavenger receptor class B type I and pitavastatin (NK-104)." in: Circulation, Vol. 110, Issue 22, pp. 3472-9, 2004 (PubMed).

Background publications Hullinger, Panek, Xu et al.: "p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain." in: The Journal of biological chemistry, Vol. 276, Issue 50, pp. 46807-14, 2001 (PubMed).

Witt, Kolleck, Fechner et al.: "Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells." in: Journal of lipid research, Vol. 41, Issue 12, pp. 2009-16, 2001 (PubMed).

Catalog No. ABIN152882

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