Scavenger Receptor Class B, Member 1 (SCARB1) antibody

Antigen: Scavenger Receptor Class B, Member 1 (SCARB1)

Synonyms: CLA1, SRB1, CLA-1, SR-BI, CD36L1, MGC138242, CD36, Cla1, SRBI, Srb1, Cla-1, SR-B1, Cd36l1, mSR-BI, AI120173, D5Ertd460e, SCARB1, cb1015, Scarb1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN152882

Additional Information

Alternative name: SCARB1

Gene ID: 949, 20778, 25073

UniProt: Q8WTV0

Immunogen: A C-terminal peptide containing residues from mouse Scavenger Receptor-BI (withinresidues 450-509).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and theirplasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BIbinds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDLwith high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues,and mediates selective cholesterol uptake by a distinct mechanism.In mice, it seems that SR-BI plays a key role in determining the levels of plasmalipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland. Alternate Names: anti-SCARB1 antibody, anti-SR-B1 antibody, anti-SR-BI antibody, anti-ScavengerReceptor-BI antibody, anti-Scavenger Receptor-B1 antibody, anti-HCLA 1 antibody,anti-SRBI antibody, anti-SR BI antibody, anti-SRB1 antibody, anti-CD36 and LIMPIIanalogous 1 antibody, anti-CD36 antigen antibody, anti-CD36L1 antibody, anti-CLA1antibody, anti-CLA 1 antibody, anti-Collagen type I receptor, anti-thrombospondinreceptor-like 1 antibody, anti-Scavenger Receptor Class B Type 1 antibody,anti-scavenger receptor class B type 1 antibody, anti-scavenger receptor class B type IIIantibody, anti-scavenger receptor class B, member 1 antibody, anti-MGC138242antibody.

Specificity: 400-104 is specific for the SR-BI. Species Reactivity: Reacts with mouse, rat, mink and hamster SR-BI and the long form of human CLA-I.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:1000 -1:5000

Concentration: 3.0 mg/ml

Purification: Purified

Buffer: Tris-citrate/phosphate [pH 7-8] buffer.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Detection of SR-BI in 25 ug of mouse liver lysate, using ABIN152882 (lotJ) at a 1:1,000 dilution, shows distinct band at 82 kDa.

anti-Scavenger Receptor Class B, Member 1 (SCARB1) antibody (Image 2)

Immunolocalization of SR-BI in adult mink testis using ABIN152882. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal.

Publications

Witt, Kolleck, Fechner et al.: "Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells." in: Journal of lipid research, Vol. 41, Issue 12, pp. 2009-16, 2001 (PubMed).

Hullinger, Panek, Xu et al.: "p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain." in: The Journal of biological chemistry, Vol. 276, Issue 50, pp. 46807-14, 2001 (PubMed).

Han, Parsons, Zhou et al.: "Functional interplay between the macrophage scavenger receptor class B type I and pitavastatin (NK-104)." in: Circulation, Vol. 110, Issue 22, pp. 3472-9, 2004 (PubMed).

Brodeur, Luangrath, Bourret et al.: "Physiological importance of SR-BI in the in vivo metabolism of human HDL and LDL in male and female mice." in: Journal of lipid research, Vol. 46, Issue 4, pp. 687-96, 2005 (PubMed).

Lemieux, Guelinas, Lalonde et al.: "Hypolipidemic action of the SERM acolbifene is associated with decreased liver MTP and increased SR-BI and LDL receptors." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1285-94, 2005 (PubMed).

Lalanne, Lambert, Amar et al.: "Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1312-9, 2005 (PubMed).

Johansson, Rudling, Scanlan et al.: "Selective thyroid receptor modulation by GC-1 reduces serum lipids and stimulates steps of reverse cholesterol transport in euthyroid mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10297-302, 2005 (PubMed).

Harder, Vassiliou, McBride et al.: "Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy." in: Journal of lipid research, Vol. 47, Issue 3, pp. 492-503, 2006 (PubMed).

Yesilaltay, Morales, Amigo et al.: "Effects of hepatic expression of the high-density lipoprotein receptor SR-BI on lipoprotein metabolism and female fertility." in: Endocrinology, Vol. 147, Issue 4, pp. 1577-88, 2006 (PubMed).

Thomuas-Moyua, Gianotti, Proenza et al.: "The age-related paraoxonase 1 response is altered by long-term caloric restriction in male and female rats." in: Journal of lipid research, Vol. 47, Issue 9, pp. 2042-8, 2006 (PubMed).

Sanue, Sinnett, Delvin et al.: "Localization and role of NPC1L1 in cholesterol absorption in human intestine." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2112-20, 2006 (PubMed).

Akpovi, Yoon, Vitale et al.: "The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2233-47, 2006 (PubMed).

Azhar, Medicherla, Shen et al.: "LDL and cAMP cooperate to regulate the functional expression of the LRP in rat ovarian granulosa cells." in: Journal of lipid research, Vol. 47, Issue 11, pp. 2538-50, 2006 (PubMed).

Zhong, Gastaminza, Chung et al.: "Persistent hepatitis C virus infection in vitro: coevolution of virus and host." in: Journal of virology, Vol. 80, Issue 22, pp. 11082-93, 2006 (PubMed).

Wadsack, Tabano, Maier et al.: "Intrauterine growth restriction is associated with alterations in placental lipoprotein receptors and maternal lipoprotein composition." in: American journal of physiology. Endocrinology and metabolism, Vol. 292, Issue 2, pp. E476-84, 2007 (PubMed).

Timmers, Sharma, Opavsky et al.: "E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop." in: Molecular and cellular biology, Vol. 27, Issue 1, pp. 65-78, 2006 (PubMed).

Qu, Perdomo, Su et al.: "Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice." in: Journal of lipid research, Vol. 48, Issue 7, pp. 1476-87, 2007 (PubMed).

During, Harrison: "Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells." in: Journal of lipid research, Vol. 48, Issue 10, pp. 2283-94, 2007 (PubMed).

Yvan-Charvet, Pagler, Wang et al.: "SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL." in: Journal of lipid research, Vol. 49, Issue 1, pp. 107-14, 2007 (PubMed).

Valiyaveettil, Kar, Ashraf et al.: "Oxidized high-density lipoprotein inhibits platelet activation and aggregation via scavenger receptor BI." in: Blood, Vol. 111, Issue 4, pp. 1962-71, 2008 (PubMed).

Eacker, Agrawal, Qian et al.: "Hormonal regulation of testicular steroid and cholesterol homeostasis." in: Molecular endocrinology (Baltimore, Md.), Vol. 22, Issue 3, pp. 623-35, 2008 (PubMed).

Tietge, Nijstad, Havinga et al.: "Secretory phospholipase A2 increases SR-BI-mediated selective uptake from HDL but not biliary cholesterol secretion." in: Journal of lipid research, Vol. 49, Issue 3, pp. 563-71, 2008 (PubMed).

Barth, Schnober, Neumann-Haefelin et al.: "Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells." in: Journal of virology, Vol. 82, Issue 7, pp. 3466-79, 2008 (PubMed).

Montoudis, Seidman, Boudreau et al.: "Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake." in: Journal of lipid research, Vol. 49, Issue 5, pp. 961-72, 2008 (PubMed).

Ashraf, Kar, Chen et al.: "Specific oxidized phospholipids inhibit scavenger receptor bi-mediated selective uptake of cholesteryl esters." in: The Journal of biological chemistry, Vol. 283, Issue 16, pp. 10408-14, 2008 (PubMed).

Drover, Nguyen, Bastie et al.: "CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 13108-15, 2008 (PubMed).