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SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term)

Details for Product anti-SCARB1 Antibody No. ABIN152882, Supplier: Log in to see
Antigen
  • AI120173
  • cb1015
  • CD36
  • CD36L1
  • Cd36l1
  • CLA-1
  • Cla-1
  • CLA1
  • Cla1
  • D5Ertd460e
  • Hdlq1
  • HDLQTL6
  • Hlb398
  • mSR-BI
  • SCARB1
  • Scarb1
  • SR-B1
  • SR-BI
  • Srb1
  • SRB1
  • SRBI
Epitope
C-Term, AA 450-509
50
27
23
17
15
2
2
1
1
1
1
1
1
Reactivity
Chinese Hamster, Golden Syrian Hamster, Hamster, Human, Mouse (Murine), Mustelid, Primate, Rabbit, Rat (Rattus)
99
70
65
37
25
25
24
13
13
12
12
12
1
Host
Rabbit
78
24
15
Clonality
Polyclonal
Conjugate
This SCARB1 antibody is un-conjugated
6
6
5
5
4
4
4
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
99
52
45
41
36
31
30
22
13
9
6
1
1
1
1
Supplier
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Immunogen A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Cross-Reactivity (Details) Reactivity with CHO cells reported in scientific literature (PMID: 12356718).
Purification Immunogen affinity purified
Alternative Name SR-BI (SCARB1 Antibody Abstract)
Background Gene Symbol: SCARB1
Molecular Weight Theoretical MW: 82 kDa
Gene ID 949, 20778, 25073
UniProt Q61009
Application Notes Western Blot 1:1000-1:5000, Simple Western 1:100, Flow Cytometry 1:10-1:1000, Immunohistochemistry 2.5-5 μg/mL, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 2.5-5 μg/mL, Immunohistochemistry-Frozen
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for SR-BI Antibody Procedure Guide for NB 400-104 Polyclonal anti-SR-B1Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:250 dilution of NB400-104 (anti-SR-BI), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts were used as a positive control for this antibody.FACS Procedure
. Resuspend 1x10^6 cells in FACS
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Detection of SR-BI in 25 ug of mouse liver lysate, using ABIN152882 (lotJ) at a 1:1,0...
Flow Cytometry (FACS) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) anti-Scavenger Receptor Class B, Member 1 (SCARB1) (AA 450-509), (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunolocalization of SR-BI in adult mink testis using ABIN152882. SR-BI labeling is ...
Simple Western (SimWes) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Simple Western: SR-BI Antibody [ABIN152882] - Simple Western lane view shows a specif...
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Western Blot: SR-BI Antibody [ABIN152882] - SR-BI antibody was tested in human adrena...
Flow Cytometry (FACS) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Flow Cytometry: SR-BI Antibody [ABIN152882] - Analysis using the DyLight 550 conjugat...
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Western Blot: SR-BI Antibody [ABIN152882] - Detection of SR-BI in rat H4IIE total cel...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunohistochemistry-Paraffin: SR-BI Antibody [ABIN152882] - IHC analysis of a formal...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunohistochemistry-Paraffin: SR-BI Antibody [ABIN152882] - IHC analysis of a formal...
Immunohistochemistry (IHC) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunohistochemistry: SR-BI Antibody [ABIN152882] - Staining of adrenal cortex.
Immunofluorescence (IF) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunocytochemistry/Immunofluorescence: SR-BI Antibody [ABIN152882] - SRB1 antibody w...
Product cited in: Mysore, Zhou, Sädevirta, Savolainen-Peltonen, Nidhina Haridas, Soronen, Leivonen, Sarin, Fischer-Posovszky, Wabitsch, Yki-Järvinen, Olkkonen: "MicroRNA-192* impairs adipocyte triglyceride storage." in: Biochimica et biophysica acta, Vol. 1861, Issue 4, pp. 342-51, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Shi, Mao, Zhong, Liu, Zhao, Yu, Zhu, Wei, Zhu, Sun, Mao, Zeng: "Lanatoside C Promotes Foam Cell Formation and Atherosclerosis." in: Scientific reports, Vol. 6, pp. 20154, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hong, Xu, Huang, Lei, Xu, Zhao, Yu: "Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency." in: Scientific reports, Vol. 6, pp. 20503, 2016 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ganesan, Mates, Cheplowitz, Avila, Zimmerer, Yao, Maiseyeu, Rajaram, Robinson, Anderson: "Scavenger receptor B1, the HDL receptor, is expressed abundantly in liver sinusoidal endothelial cells." in: Scientific reports, Vol. 6, pp. 20646, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Cheng, Li, Huang, Ma, Wu, Jiang, Li, Li, Han, Li, Chen, Wang, Song, Peng, Jiang: "CD36 is a co-receptor for hepatitis C virus E1 protein attachment." in: Scientific reports, Vol. 6, pp. 21808, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Julovi, Xue, Thanh LE, Gill, Bulanadi, Patel, Waddington, Rye, Moghaddam, Smith: "Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151475, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Li, Wang, Li, Yin, Li, Zhang: "Up-regulated expression of scavenger receptor class B type 1 (SR-B1) is associated with malignant behaviors and poor prognosis of breast cancer." in: Pathology, research and practice, Vol. 212, Issue 6, pp. 555-9, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Song, Wu, Zhang, Yu, Yang, Jiao, Wang, Song, Wu, Zhang, Liu, Qin: "High-density lipoprotein inhibits ox-LDL-induced adipokine secretion by upregulating SR-BI expression and suppressing ER Stress pathway." in: Scientific reports, Vol. 6, pp. 30889, 2016 (PubMed). Further details: Western Blotting

Geng, Chen, Yuan, Peng, Maitra, Diao, Chen, Zhang, Hu, Qi, Pierce, Ling, Xiong, Li: "The persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis." in: Nature communications, Vol. 7, pp. 13436, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Cedó, García-León, Baila-Rueda, Santos, Grijalva, Martínez-Cignoni, Carbó, Metso, López-Vilaró, Zorzano, Valledor, Cenarro, Jauhiainen, Lerma, Fogelman, Reddy, Escolà-Gil, Blanco-Vaca: "ApoA-I mimetic administration, but not increased apoA-I-containing HDL, inhibits tumour growth in a mouse model of inherited breast cancer." in: Scientific reports, Vol. 6, pp. 36387, 2016 (PubMed). (Sample species: Mouse (Murine)).

Wang, Palme, Rotter, Schilcher, Cukaj, Wang, Ladurner, Heiss, Stangl, Dirsch, Atanasov: "Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages." in: Molecular nutrition & food research, 2016 (PubMed).

Ng, Schwartz, March, Galstian, Gural, Shan, Prabhu, Mota, Bhatia: "Human iPSC-derived hepatocyte-like cells support Plasmodium liver-stage infection in vitro." in: Stem cell reports, Vol. 4, Issue 3, pp. 348-59, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Wang, Meng, Wei, Shi, Mao: "Expression and regulation of scavenger receptor class B type 1 in the rat ovary and uterus during the estrous cycle." in: Acta histochemica, Vol. 117, Issue 3, pp. 297-304, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Sané, Seidman, Spahis, Lamantia, Garofalo, Montoudis, Marcil, Levy: "New Insights In Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis." in: Journal of cellular biochemistry, Vol. 116, Issue 10, pp. 2270-82, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Yan, Shen, Jiang, Tang, Wang, Li, Ling: "Coenzyme Q10 consumption promotes ABCG1-mediated macrophage cholesterol efflux: a randomized, double-blind, placebo-controlled, cross-over study in healthy volunteers." in: Molecular nutrition & food research, Vol. 59, Issue 9, pp. 1725-34, 2015 (PubMed).

Calattini, Fusil, Mancip, Dao Thi, Granier, Gadot, Scoazec, Zeisel, Baumert, Lavillette, Dreux, Cosset: "Functional and Biochemical Characterization of Hepatitis C Virus (HCV) Particles Produced in a Humanized Liver Mouse Model." in: The Journal of biological chemistry, Vol. 290, Issue 38, pp. 23173-87, 2015 (PubMed).

Bartels, Christoffersen, Lindholm, Nielsen: "Altered metabolism of LDL in the arterial wall precedes atherosclerosis regression." in: Circulation research, Vol. 117, Issue 11, pp. 933-42, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Nadeem, Thomas, Ulf, Elena, Anggakusuma, Mohamed, Frank, Patrick: "Cell culture-derived HCV cannot infect synovial fibroblasts." in: Scientific reports, Vol. 5, pp. 18043, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Cameron, Morrell, Bao, Swaim, Rodriguez, Lowenstein: "A Novel Anti-Inflammatory Effect for High Density Lipoprotein." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144372, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Reboul, Soayfane, Goncalves, Cantiello, Bott, Nauze, Tercé, Collet, Coméra: "Respective contributions of intestinal Niemann-Pick C1-like 1 and scavenger receptor class B type I to cholesterol and tocopherol uptake: in vivo v. in vitro studies." in: The British journal of nutrition, Vol. 107, Issue 9, pp. 1296-304, 2014 (PubMed).