SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term)

Details for Product anti-SCARB1 Antibody No. ABIN152882, Supplier: Log in to see
Antigen
  • cb1015
  • SCARB1
  • Scarb1
  • CD36L1
  • CLA-1
  • CLA1
  • HDLQTL6
  • SR-BI
  • SRB1
  • AI120173
  • CD36
  • Cd36l1
  • Cla-1
  • Cla1
  • D5Ertd460e
  • Hdlq1
  • Hlb398
  • SR-B1
  • SRBI
  • Srb1
  • mSR-BI
  • scavenger receptor class B, member 1
  • scavenger receptor class B member 1
  • scarb1
  • SCARB1
  • Scarb1
  • CpipJ_CPIJ014332
  • CpipJ_CPIJ019101
Alternatives
anti-Rat (Rattus) SCARB1 antibody for Blocking Peptide
Epitope
C-Term, AA 450-509
50
27
17
16
15
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Chinese Hamster, Golden Syrian Hamster, Hamster, Human, Mouse (Murine), Mustelid, Primate, Rabbit, Rat (Rattus)
102
75
73
37
25
25
24
15
14
13
12
12
2
2
1
Host
Rabbit
80
26
16
Clonality
Polyclonal
Conjugate
This SCARB1 antibody is un-conjugated
6
6
5
5
4
4
4
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
103
55
44
43
41
41
31
29
12
6
3
3
1
1
1
1
Options
Supplier
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Immunogen A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Purification Immunogen affinity purified
Alternative Name SR-BI (SCARB1 Antibody Abstract)
Background Gene Symbol: SCARB1
Molecular Weight Theoretical MW: 82 kDa
Gene ID 949
UniProt Q61009
Pathways Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Application Notes Western Blot 1:1000-1:5000, Simple Western 1:100, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 2.5-5 μg/mL, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 2.5-5 μg/mL, Immunohistochemistry-FrozenIn Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26865459). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for SR-BI Antibody Procedure Guide for NB 400-104 Polyclonal anti-SR-B1Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:250 dilution of NB400-104 (anti-SR-BI), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts were used as a positive control for this antibody.FACS Procedure
. Resuspend 1x10^6 cells in FACS
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Detection of SR-BI in 25 ug of mouse liver lysate, using ABIN152882 (lotJ) at a 1:1,0...
Flow Cytometry (FACS) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) anti-Scavenger Receptor Class B, Member 1 (SCARB1) (AA 450-509), (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunolocalization of SR-BI in adult mink testis using ABIN152882. SR-BI labeling is ...
Simple Western (SimWes) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Simple Western: SR-BI Antibody [ABIN152882] - Simple Western lane view shows a specif...
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Western Blot: SR-BI Antibody [ABIN152882] - SR-BI antibody was tested in human adrena...
Flow Cytometry (FACS) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Flow Cytometry: SR-BI Antibody [ABIN152882] - Analysis using the DyLight 550 conjugat...
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Western Blot: SR-BI Antibody [ABIN152882] - Detection of SR-BI in rat H4IIE total cel...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunohistochemistry-Paraffin: SR-BI Antibody [ABIN152882] - IHC analysis of a formal...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunohistochemistry-Paraffin: SR-BI Antibody [ABIN152882] - IHC analysis of a formal...
Immunohistochemistry (IHC) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunohistochemistry: SR-BI Antibody [ABIN152882] - Staining of adrenal cortex.
Immunofluorescence (IF) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (C-Term) (ABIN152882) Immunocytochemistry/Immunofluorescence: SR-BI Antibody [ABIN152882] - SRB1 antibody w...
Product cited in: Mysore, Zhou, Sädevirta, Savolainen-Peltonen, Nidhina Haridas, Soronen, Leivonen, Sarin, Fischer-Posovszky, Wabitsch, Yki-Järvinen, Olkkonen: "MicroRNA-192* impairs adipocyte triglyceride storage." in: Biochimica et biophysica acta, Vol. 1861, Issue 4, pp. 342-51, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Shi, Mao, Zhong, Liu, Zhao, Yu, Zhu, Wei, Zhu, Sun, Mao, Zeng: "Lanatoside C Promotes Foam Cell Formation and Atherosclerosis." in: Scientific reports, Vol. 6, pp. 20154, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hong, Xu, Huang, Lei, Xu, Zhao, Yu: "Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency." in: Scientific reports, Vol. 6, pp. 20503, 2016 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ganesan, Mates, Cheplowitz, Avila, Zimmerer, Yao, Maiseyeu, Rajaram, Robinson, Anderson: "Scavenger receptor B1, the HDL receptor, is expressed abundantly in liver sinusoidal endothelial cells." in: Scientific reports, Vol. 6, pp. 20646, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Cheng, Li, Huang, Ma, Wu, Jiang, Li, Li, Han, Li, Chen, Wang, Song, Peng, Jiang: "CD36 is a co-receptor for hepatitis C virus E1 protein attachment." in: Scientific reports, Vol. 6, pp. 21808, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Julovi, Xue, Thanh LE, Gill, Bulanadi, Patel, Waddington, Rye, Moghaddam, Smith: "Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151475, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Li, Wang, Li, Yin, Li, Zhang: "Up-regulated expression of scavenger receptor class B type 1 (SR-B1) is associated with malignant behaviors and poor prognosis of breast cancer." in: Pathology, research and practice, Vol. 212, Issue 6, pp. 555-9, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Song, Wu, Zhang, Yu, Yang, Jiao, Wang, Song, Wu, Zhang, Liu, Qin: "High-density lipoprotein inhibits ox-LDL-induced adipokine secretion by upregulating SR-BI expression and suppressing ER Stress pathway." in: Scientific reports, Vol. 6, pp. 30889, 2016 (PubMed). Further details: Western Blotting

Geng, Chen, Yuan, Peng, Maitra, Diao, Chen, Zhang, Hu, Qi, Pierce, Ling, Xiong, Li: "The persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis." in: Nature communications, Vol. 7, pp. 13436, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Cedó, García-León, Baila-Rueda, Santos, Grijalva, Martínez-Cignoni, Carbó, Metso, López-Vilaró, Zorzano, Valledor, Cenarro, Jauhiainen, Lerma, Fogelman, Reddy, Escolà-Gil, Blanco-Vaca: "ApoA-I mimetic administration, but not increased apoA-I-containing HDL, inhibits tumour growth in a mouse model of inherited breast cancer." in: Scientific reports, Vol. 6, pp. 36387, 2016 (PubMed). (Sample species: Mouse (Murine)).

Wang, Palme, Rotter, Schilcher, Cukaj, Wang, Ladurner, Heiss, Stangl, Dirsch, Atanasov: "Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages." in: Molecular nutrition & food research, 2016 (PubMed).

Ng, Schwartz, March, Galstian, Gural, Shan, Prabhu, Mota, Bhatia: "Human iPSC-derived hepatocyte-like cells support Plasmodium liver-stage infection in vitro." in: Stem cell reports, Vol. 4, Issue 3, pp. 348-59, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Wang, Meng, Wei, Shi, Mao: "Expression and regulation of scavenger receptor class B type 1 in the rat ovary and uterus during the estrous cycle." in: Acta histochemica, Vol. 117, Issue 3, pp. 297-304, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Sané, Seidman, Spahis, Lamantia, Garofalo, Montoudis, Marcil, Levy: "New Insights In Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis." in: Journal of cellular biochemistry, Vol. 116, Issue 10, pp. 2270-82, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Yan, Shen, Jiang, Tang, Wang, Li, Ling: "Coenzyme Q10 consumption promotes ABCG1-mediated macrophage cholesterol efflux: a randomized, double-blind, placebo-controlled, cross-over study in healthy volunteers." in: Molecular nutrition & food research, Vol. 59, Issue 9, pp. 1725-34, 2015 (PubMed).

Calattini, Fusil, Mancip, Dao Thi, Granier, Gadot, Scoazec, Zeisel, Baumert, Lavillette, Dreux, Cosset: "Functional and Biochemical Characterization of Hepatitis C Virus (HCV) Particles Produced in a Humanized Liver Mouse Model." in: The Journal of biological chemistry, Vol. 290, Issue 38, pp. 23173-87, 2015 (PubMed).

Bartels, Christoffersen, Lindholm, Nielsen: "Altered metabolism of LDL in the arterial wall precedes atherosclerosis regression." in: Circulation research, Vol. 117, Issue 11, pp. 933-42, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Nadeem, Thomas, Ulf, Elena, Anggakusuma, Mohamed, Frank, Patrick: "Cell culture-derived HCV cannot infect synovial fibroblasts." in: Scientific reports, Vol. 5, pp. 18043, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Cameron, Morrell, Bao, Swaim, Rodriguez, Lowenstein: "A Novel Anti-Inflammatory Effect for High Density Lipoprotein." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144372, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Reboul, Soayfane, Goncalves, Cantiello, Bott, Nauze, Tercé, Collet, Coméra: "Respective contributions of intestinal Niemann-Pick C1-like 1 and scavenger receptor class B type I to cholesterol and tocopherol uptake: in vivo v. in vitro studies." in: The British journal of nutrition, Vol. 107, Issue 9, pp. 1296-304, 2014 (PubMed).