LIPG antibody (Lipase, Endothelial) (C-Term)

Details for Product anti-LIPG Antibody No. ABIN152888, Supplier: Log in to see
Antigen
  • zgc:55345
  • MGC69106
  • LIPG
  • lipe
  • EDL
  • EL
  • PRO719
  • 3110013K01Rik
  • lipase
  • mEDL
  • lipase, endothelial
  • endothelial lipase
  • Endothelial lipase
  • endothelial lipase-like
  • LIPG
  • lipg
  • CpipJ_CPIJ004802
  • lipe
  • LOC509808
  • Lipg
Epitope
C-Term
21
20
15
11
7
6
5
5
4
3
3
2
2
2
1
1
1
1
Reactivity
Human
194
50
31
8
5
4
3
3
3
2
2
Host
Rabbit
113
73
16
Clonality
Polyclonal
Conjugate
This LIPG antibody is un-conjugated
9
7
7
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
162
75
64
51
41
41
28
27
26
12
3
2
2
Options
Supplier
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Immunogen A C-terminal synthetic peptide made to human Endothelial Lipase [UniProt# Q9Y5X9]
No Cross-Reactivity Mouse (Murine)
Purification Immunogen affinity purified
Alternative Name Endothelial Lipase (LIPG Antibody Abstract)
Background Gene Symbol: LIPG
Gene ID 9388
UniProt Q9Y5X9
Research Area Enzymes
Application Notes Western Blot 1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-FrozenThis Endothelial Lipase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. In Western blot a band is observed at ~57 kDa, representing the mature, non-glycosylated form of endothelial lipase protein. Another band is observed at ~30 kDa, representing the proteolytic form of the protein. In ICC/IF cytoplasmic staining was observed in HeLa cells.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Endothelial Lipase Antibody Western Blot Procedure
1. Heat diluted samples at 70 degrees Celcius for 10 minutes.
. Load samples onto a 10 % Bis-Tris gel.
. Run gel for ~45 minutes at 200V.
. Transfer gel to PVDF membrane at 45V for ~90 minutes.
. Block the membrane overnight at 4 degrees Celcius, in blocking buffer [PBS + 0.2 % Tween-20 + 5 % milk], gently shaking.
. Rinse the membrane twice, quickly, in PBS-T.
. Wash the membrane in PBS-T 3 times for 5 minutes, each.
. Incubate the membrane with primary antibody [NB 400-111 or NB 400-118] , diluted 1:500 in blocking buffer, for 1 hour at RT, gently shaking.
. Rinse the membrane twice, quickly, in PBS-T.
. Wash the membrane in PBS-T 3 times for 5 minutes, each.
. Incubate the membrane with secondary antibody [NB 730-H], diluted 1:5,000 in blocking buffer, for 1 hour at RT, gently shaking.
. Rinse the membrane twice, quickly, in PBS-T.
. Wash the membrane in PBS-T 3 times for 5 minutes, each.
. Detect protein using ECL, ~1 minute exposure.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-LIPG antibody (Lipase, Endothelial) (C-Term) (ABIN152888) Human endothelial lipase detected in transfected 293 lysates using ABIN152888 (MW 57 ...
Western Blotting (WB) image for anti-LIPG antibody (Lipase, Endothelial) (C-Term) (ABIN152888) Western Blot: Endothelial Lipase Antibody [ABIN152888] - Cells were transfected with ...
Immunofluorescence (IF) image for anti-LIPG antibody (Lipase, Endothelial) (C-Term) (ABIN152888) Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [ABIN152888] - An...
Product cited in: Yang, Kuwano, Lagor, Albert, Brenton, Rader, Ford, Brown: "Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species." in: Lipids, Vol. 49, Issue 6, pp. 505-15, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Razzaghi, Tempczyk-Russell, Haubold, Santorico, Shokati, Christians, Churchill: "Genetic and structure-function studies of missense mutations in human endothelial lipase." in: PLoS ONE, Vol. 8, Issue 3, pp. e55716, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Essaji, Yang, Albert, Ford, Brown: "Hydrolysis products generated by lipoprotein lipase and endothelial lipase differentially impact THP-1 macrophage cell signalling pathways." in: Lipids, Vol. 48, Issue 8, pp. 769-78, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Robert, Weber, Frese, Emmert, Schmidt, von Eckardstein, Rohrer, Hoerstrup: "A three-dimensional engineered artery model for in vitro atherosclerosis research." in: PLoS ONE, Vol. 8, Issue 11, pp. e79821, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Nijstad, Wiersma, Gautier, van der Giet, Maugeais, Tietge: "Scavenger receptor BI-mediated selective uptake is required for the remodeling of high density lipoprotein by endothelial lipase." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6093-100, 2009 (PubMed). (Sample species: Human). Further details: Western Blotting

Wiersma, Gatti, Nijstad, Kuipers, Tietge: "Hepatic SR-BI, not endothelial lipase, expression determines biliary cholesterol secretion in mice." in: Journal of lipid research, Vol. 50, Issue 8, pp. 1571-80, 2009 (PubMed).