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ABCG8 antibody (ATP-Binding Cassette, Sub-Family G (WHITE), Member 8) (N-Term)

Details for Product anti-ABCG8 Antibody No. ABIN152894, Supplier: Log in to see
Antigen
  • DDBDRAFT_0167690
  • DDBDRAFT_0191232
  • DDB_0167690
  • DDB_0191232
  • zgc:172358
  • GBD4
  • STSL
  • 1300003C16Rik
  • AI114946
  • sterolin-2
  • ATP-binding cassette, sub-family G (WHITE), member 8
  • ATP-binding cassette sub-family G member 8
  • ABC transporter G family protein
  • ATP-binding cassette, subfamily G (WHITE), member 8
  • ABCG8
  • PTRG_02054
  • abcG8
  • abcg8
  • Abcg8
Epitope
N-Term
30
2
1
1
1
Reactivity
Human, Mouse (Murine)
65
39
11
Host
Rabbit
56
13
Clonality
Polyclonal
Conjugate
This ABCG8 antibody is un-conjugated
5
5
5
5
5
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
67
23
23
21
16
6
2
1
1
Options
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Immunogen A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]
Purification Immunogen affinity purified
Alternative Name ABCG8 (ABCG8 Antibody Abstract)
Background Gene Symbol: ABCG8
Molecular Weight Theoretical MW: 70 kDa
Gene ID 64241
UniProt Q9H221
Research Area Cancer, Atherosclerosis, Metabolism
Application Notes Western Blot 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:250This ABCG8 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot where a doublet is seen at ~67 kDa and ~72 kDa. Longer exposure times are needed to visualize the wild type ABCG8. For mouse, 100 μg protein (B6/129AKRFVB liver homogenate) loaded with antibody diluted at 1:500. In ICC/IF puncate membrane localization with some weak nuclear reactivity was observed in HEK293 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot protocol specific for ABCG8 Antibody Western Blot Protocol
1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8 % SDS-PAGE, reducing.
. Block the protein transferred membrane for 30 minutes in blocking buffer [5 % NFDM, 10 mM Tris (pH 7.
6) saline, 0.2 % Tween-20] at room temperature.
. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-117], 1:250 for 1 hour at room temperature.
. Wash the membrane 3x 5 minutes in blocking buffer without milk.
. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.
. Wash the membrane 3x 5 minutes in blocking buffer without milk.
. Wash the membrane for 5 minutes in TBS.
. Develop using West-Pico ECL reagent (Pierce). ICC/IF protocol specific for ABCG8 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ABCG8 antibody (ATP-Binding Cassette, Sub-Family G (WHITE), Member 8) (N-Term) (ABIN152894) Western Blot: ABCG8 Antibody [ABIN438175] - Total protein from Mouse small intestine ...
Immunofluorescence (IF) image for anti-ABCG8 antibody (ATP-Binding Cassette, Sub-Family G (WHITE), Member 8) (N-Term) (ABIN152894) Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [ABIN438175] - ABCG8 antibody ...
Product cited in: Heverin, Ali, Olin, Tillander, Joibari, Makoveichuk, Leitersdorf, Warner, Olivercrona, Gustafsson, Björkhem: "On the regulatory importance of 27-hydroxycholesterol in mouse liver." in: The Journal of steroid biochemistry and molecular biology, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Sachdev, Leopold, Bauer, Patankar, Iqbal, Obrowsky, Boverhof, Doktorova, Scheicher, Goeritzer, Kolb, Turnbull, Zimmer, Hoefler, Hussain, Groen, Kratky: "Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism." in: Biochimica et biophysica acta, Vol. 1861, Issue 9 Pt A, pp. 1132-41, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Huang, Wang, Quan, Wang, Yang, Zhong: "Lactobacillus acidophilus ATCC 4356 prevents atherosclerosis via inhibition of intestinal cholesterol absorption in apolipoprotein E-knockout mice." in: Applied and environmental microbiology, Vol. 80, Issue 24, pp. 7496-504, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Bujold, Mellal, Zoccal, Rhainds, Brissette, Febbraio, Marleau, Ong: "EP 80317, a CD36 selective ligand, promotes reverse cholesterol transport in apolipoprotein E-deficient mice." in: Atherosclerosis, Vol. 229, Issue 2, pp. 408-14, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Levy, Ben Djoudi Ouadda, Spahis, Sane, Garofalo, Grenier, Emonnot, Yara, Couture, Beaulieu, Ménard, Seidah, Elchebly: "PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells." in: Atherosclerosis, Vol. 227, Issue 2, pp. 297-306, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Grenier, Garofalo, Delvin, Levy: "Modulatory role of PYY in transport and metabolism of cholesterol in intestinal epithelial cells." in: PLoS ONE, Vol. 7, Issue 7, pp. e40992, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Mathur, Watt, Field: "Regulation of intestinal NPC1L1 expression by dietary fish oil and docosahexaenoic acid." in: Journal of lipid research, Vol. 48, Issue 2, pp. 395-404, 2007 (PubMed).

Background publications Lu, Lee, Hazard, Brooks-Wilson, Hidaka, Kojima, Ose, Stalenhoef, Mietinnen, Bjorkhem, Bruckert, Pandya, Brewer, Salen, Dean, Srivastava, Patel: "Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8, respectively." in: American journal of human genetics, Vol. 69, Issue 2, pp. 278-90, 2001 (PubMed).

Garcia, Wilund, Arca, Zuliani, Fellin, Maioli, Calandra, Bertolini, Cossu, Grishin, Barnes, Cohen, Hobbs: "Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein." in: Science (New York, N.Y.), Vol. 292, Issue 5520, pp. 1394-8, 2001 (PubMed).

Schmitz, Langmann, Heimerl: "Role of ABCG1 and other ABCG family members in lipid metabolism." in: Journal of lipid research, Vol. 42, Issue 10, pp. 1513-20, 2001 (PubMed).