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SCARB1 antibody (Scavenger Receptor Class B, Member 1) (AA 230-380)

Details for Product anti-SCARB1 Antibody No. ABIN152901, Supplier: Login to see New
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AA 230-380, Extracellular Domain
(23), (15), (9), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(58), (32), (27), (2)
(54), (15), (7)
This SCARB1 antibody is un-conjugated
(4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(59), (26), (24), (20), (9), (7), (6), (6), (5), (2), (2), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mL
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Immunogen A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.
Cross-Reactivity (Details) Previous lots have been shown to react with rat,and human as well.
Purification Whole antisera
Alternative Name SCARB1 (SCARB1 Antibody Abstract)
Background High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and theirplasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI andSR-BII (previously known as SR-BI.2) are the alternatively spliced products of a singlegene. SR-BII and SR-BI are identical except for the encoded c-terminal cytoplasmicdomain. Both SR-BI and SR-BII bind HDL and mediates selective uptake of HDLcholesteryl ester, but with SR-BII having an approximately 4-fold lower efficiency thanSR-BI. SR-BI and SR-BII are expressed primarily in liver and non-placental steroidgenictissues.Although the role of these scavenger receptors is not completely clear, SR-BII mRNAresults from the alternative splicing of SR-BI precursor transcripts with both isoformsmediating selective transfer of lipid between HDL and cells. Therefore, the relativeexpression and functional activities of these two isoforms create a potential means ofregulating selective lipid transfer between HDL and cells. Alternate Names: anti-SR-B1/B2 antibody, anti-SR-BI/BII antibody, anti-Scavenger Receptor-BI/BIIantibody, anti-Scavenger Receptor-B1/B2 antibody, anti-RED1 antibody, anti-RED 1antibody, anti-RED-1 antibody. Related Diseases: Cancer, Coronary Artery Disease, Atherosclerosis
Gene Symbol: SCARB1
Gene ID 949
Application Notes This SR-BI/SR-BII antibody is useful for Flow Cytometry (PMID: 22097902), functional studies (customer feedback), Immunocytochemistry, Western blot and Immunohistochemistry. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI and/or SR-BII such as liver, ovary, adrenal glands, and to as lesser extent testes, heart and mammary glands.
Recommended dilutions: Flow Cytometry 1:400, Functional, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry 1:10-1:500, Immunoprecipitation, Western Blot 1:1000
Protocol Western blot Protocol for SR-BI/SR-BII Antibody Western Blot Procedure
1. Run 80 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:1,000 dilution of NB400-134 (anti-SR-BI/BII), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with dilution of goat anti-rabbit IgG-HRP (secondary), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Chemiluminescence reagents. ICC/IF Protocol for SR-BI/SR-BII Antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (AA 230-380) (ABIN152901) anti-Scavenger Receptor Class B, Member 1 (SCARB1) (AA 230-380), (Extracellular Domain) antibody
Product cited in: Harder, Meng, Rippstein et al.: "SR-BI undergoes cholesterol-stimulated transcytosis to the bile canaliculus in polarized WIF-B cells." in: The Journal of biological chemistry, Vol. 282, Issue 2, pp. 1445-55, 2007 (PubMed).

Kocher, Yesilaltay, Cirovic et al.: "Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52820-5, 2003 (PubMed).

Silver: "A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34042-7, 2002 (PubMed).

Catalog No. ABIN152901

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