MLX Interacting Protein-Like (MLXIPL) antibody

Antigen: MLX Interacting Protein-Like (MLXIPL)

Synonyms: ChREBP, WS-bHLH, Wbscr14, bHLHd14, MLXIPL

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN152902

Additional Information

Alternative name: MLXIPL

Gene ID: 51085, 58805, 171078

Immunogen: An C-terminal synthetic peptide made to the human ChREBP protein sequence(between residues 800-852). [NCBI# NP_116569.1, isoform 1/alpha].

Cross-Reactivity: Human, Rat (Rattus)

Description: ChREBP is a transcription factor involved in activating genes that encode enzymes offatty acid biosynthesis in liver and adipose. This protein may have relevance to lipidmetabolism, obesity, and type II diabetes. Alternate Names: anti-WBSCR14 antibody, anti-Williams Beuren Syndrome Chromosome Region 14antibody, anti-MIO antibody, anti-MONDOB antibody, anti-WS-bHLH antibody. Related Diseases: Williams-Beuren syndrome, Cancer

Specificity: This antibody is specific for ChREBP. Species Reactivity: This antibody reacts with human, mouse (see Dentin et al) and rat ChREBP. There is83% homology of the immunogen with the mouse ChREBP protein. Other species havenot been tested.

Application Details

Application Notes: Suggested working dilutions: chromatin immunoprecipitation see #5,immunofluorescence 1:100-1:500

Concentration: 1.2 mg/ml

Purification: Purified

Buffer: Preservative: 0.1% sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Detection of ChREBP in 20 ug of human hepatocyte lysate using ABIN152902. 5-10 second film exposure.

Immunofluorescent staining of a human hepatocyte using ABIN152902. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).

Detection of ChREBP in liver nuclear extracts from well-fed rats. 7% SDS gel, 1:1,000 dilution of ABIN152902. Photo courtesy of Dr. Uyeda, UT Southwestern University.

Publications

Xu, Christian, Jump: "Regulation of rat hepatic L-pyruvate kinase promoter composition and activity by glucose, n-3 polyunsaturated fatty acids, and peroxisome proliferator-activated receptor-alpha agonist." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18351-62, 2006 (PubMed).

Wang, Botolin, Xu et al.: "Regulation of hepatic fatty acid elongase and desaturase expression in diabetes and obesity." in: Journal of lipid research, Vol. 47, Issue 9, pp. 2028-41, 2006 (PubMed).

Dentin, Benhamed, Hainault et al.: "Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice." in: Diabetes, Vol. 55, Issue 8, pp. 2159-70, 2006 (PubMed).

Erhuma, Salter, Sculley et al.: "Prenatal exposure to a low-protein diet programs disordered regulation of lipid metabolism in the aging rat." in: American journal of physiology. Endocrinology and metabolism, Vol. 292, Issue 6, pp. E1702-14, 2007 (PubMed).

Aneja, Guha, Shilpi et al.: "The presence of distal and proximal promoters for rat mitochondrial glycerol-3-phosphate acyltransferase." in: Archives of biochemistry and biophysics, Vol. 470, Issue 1, pp. 35-43, 2008 (PubMed).