Apolipoprotein A-V (APOA5) antibody

Antigen: Apolipoprotein A-V (APOA5)

Synonyms: RAP3, Apoav, 1300007O05Rik, APOAV, APOA-V, MGC126836, MGC126838, MGC108612, APOA5, MGC140487, rap3, apoav, apoa-v

Clonality: Monoclonal (4H8H8E2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Catalog no.: ABIN152905

Additional Information

Alternative name: Apolipoprotein A V (ApoA V)

Gene ID: 116519

UniProt: Q6Q788

Immunogen: Ni-NTA purified full length recombinant human APOA5 expressed in E. Coli strain BL21(DE3).

Cross-Reactivity: Human

Format: Ascites

Isotype: IgG1

Clone: 4H8H8E2

Description: Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serumtriglyceride concentrations. An ApoA5 mouse knock-out model produced anapproximately four fold increase in serum triglycerides, whereas a knock-in model withhuman ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. Inaddition, peroxisome proliferator-activated receptor-4 (PPAR-alpha) agonists, which areused clinically to lower serum triglyceride concentrations, cause increased ApoA5mRNA expression. Recently, it was demonstrated that ApoA5 ispresent in human serum detected by polyclonal antibodies against both the NH2 andCOOH termini, although at much lower concentration than other apolipoproteins. Alternate Names: anti-ApoA5 antibody, anti-Apolipoprotein A5 antibody, anti-Apo A-V antibody,anti-Apolipoprotein A-V antibody, anti-Apo-A5 antibody, anti-ApoA-V antibody,anti-Apolipoprotein A V antibody. Related Diseases: Coronary Artery Disease, Hypertriglyceridemia

Specificity: Species Reactivity: Reacts with human. 83% sequence identity with Bovine, 79% sequence identity withRat, and 78% sequence identity with Mouse proteins. Theoretical Molecular Weight: 41 kDa.

Application Details

Application Notes: This antibody has been tested in ELISA. The MW of this protein is ~41 kDa. Suggested working dilutions: *Immunohistochemistry - ND*The investigator should determine the optimal working dilution for a specific application.

Concentration: 1 mg/ml

Purification: Ascites

Buffer: 0.1 ml of crude ascites (1 mg/ml) containing 0.1% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Metabolism

Restrictions: For Research Use only

Publications

Pennacchio, Olivier, Hubacek et al.: "An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing." in: Science (New York, N.Y.), Vol. 294, Issue 5540, pp. 169-73, 2001 (PubMed).