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CENPF antibody (Centromere Protein F) (C-Term)

Details for Product anti-CENPF Antibody No. ABIN152930, Supplier: Log in to see
Antigen
  • LOC100222985
  • 6530404A22Rik
  • AI325968
  • CENF
  • Lek1
  • PRO1779
  • mitosin
  • im:7140452
  • im:7144238
  • im:7154719
  • im:7156761
  • si:ch211-152h14.2
  • hcp-1
  • CMF1
  • centromere protein F
  • centromere protein F, (mitosin)
  • centromere protein F, 350/400kDa
  • centromere protein F, 350/400kDa (mitosin)
  • LOC100222985
  • LOC100343995
  • Cenpf
  • cenpf
  • CENPF
Alternatives
anti-Human CENPF antibody for Immunocytochemistry
Epitope
C-Term
34
7
3
2
2
1
1
1
1
Reactivity
Cow (Bovine), Human
66
15
3
3
1
1
Host
Rabbit
34
17
16
Clonality
Polyclonal
Conjugate
This CENPF antibody is un-conjugated
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
35
29
19
19
17
16
13
11
3
3
1
1
1
Options
Supplier
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Immunogen A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
Purification Immunogen affinity purified
Alternative Name CENPF (CENPF Antibody Abstract)
Background Gene Symbol: CENPF
Gene ID 1063
UniProt P49454
Research Area Cell Cycle, Chromatin and Nuclear Signaling
Pathways Chromatin Binding, M Phase
Application Notes Western Blot 1:1500-1:2500, Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunoprecipitation 1:50This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot where a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for CENPF Antibody Western Blot Procedure1) Resolve aliquots (30 mg) of total HeLa cell lysate on a 8.5 % lo-crosslinker gel (110:1, acryl:bis) for efficient separation and transfer. 2) Transfer to nitrocellulose membranes in 20 mM Tris-HCL (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol. Transfer time for a 1 mm thick gel is 1 hour 15 minutes at 450mA. 3) Block membranes for 1 hour with 5 % (vol/vol) nonfat dry milk/TBS-T (20 mM Tris-HCL, pH 7.6/ 137 mM NaCl/0.1 % TWEEN 20)4) Incubate membranes for 1 hour at room temperature (RT) in NB 500-101 diluted 1:3000 in nonfat dry milk/TBS-T. 5) Wash 3X30 minutes at RT with TBS-T. 6) Incubate membranes with alkaline phosphatase conjugated anti-rabbit IgG for 1 hour (RT).
7) Wash 3 x 30 minutes at RT with TBS-T8) Develop with ECL reagents (Amersham) and autoradiography.
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/mL
Buffer PBS and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-CENPF antibody (Centromere Protein F) (C-Term) (ABIN152930) Detection of CENP-F using ABIN152930 (lot D).
Flow Cytometry (FACS) image for anti-CENPF antibody (Centromere Protein F) (C-Term) (ABIN152930) Flow Cytometry: CENPF Antibody [ABIN152930] - Detection of CENPF in Hela cells using ...
Immunofluorescence (IF) image for anti-CENPF antibody (Centromere Protein F) (C-Term) (ABIN152930) Immunocytochemistry/Immunofluorescence: CENPF Antibody [ABIN152930] - Confocal immuno...
Immunofluorescence (IF) image for anti-CENPF antibody (Centromere Protein F) (C-Term) (ABIN152930) Immunocytochemistry/Immunofluorescence: CENPF Antibody [ABIN152930] - CENPF antibody ...
Product cited in: Magidson, He, Ault, OConnell, Yang, Tikhonenko, McEwen, Sui, Khodjakov: "Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells." in: The Journal of cell biology, Vol. 212, Issue 3, pp. 307-19, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Ma, Hou, Zhang, Sun, Schedl, Moley, Wang: "Rab5a is required for spindle length control and kinetochore-microtubule attachment during meiosis in oocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 9, pp. 4026-35, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Whole Mount),Western Blotting

Lee, Lee, Lee: "CaMKKβ-AMPKα2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 4, pp. 598-611, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Nakajima, Hagiwara, Oike, Okayasu, Murakami, Nakano, Shibata: "Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122582, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Bee, Nasca, Zanolini, Cendron, dAdamo, Costa, Lamperti, Celotti, Ghezzi, Zeviani: "A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy." in: EMBO molecular medicine, Vol. 7, Issue 7, pp. 918-29, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Nishikawa, Goto, Kurozumi, Matsushita, Enokida, Kojima, Naya, Nakagawa, Ichikawa, Seki: "MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer." in: International journal of urology : official journal of the Japanese Urological Association, Vol. 22, Issue 9, pp. 867-77, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Kaida, Miura: "Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells." in: Cancer science, Vol. 106, Issue 10, pp. 1370-6, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Guo, Zheng: "Lamins position the nuclear pores and centrosomes by modulating dynein." in: Molecular biology of the cell, Vol. 26, Issue 19, pp. 3379-89, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Kanfer, Courthéoux, Peterka, Meier, Soste, Melnik, Reis, Aspenström, Peter, Picotti, Kornmann: "Mitotic redistribution of the mitochondrial network by Miro and Cenp-F." in: Nature communications, Vol. 6, pp. 8015, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Zhu, Wang, Wang, Wang, Hua, Su, Brako, Garcia-Barrio, Ye, Wei, Zou, Ding, Liu, Liu, Yao: "Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis." in: The Journal of biological chemistry, Vol. 290, Issue 45, pp. 27053-66, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Magidson, Paul, Yang, Ault, OConnell, Tikhonenko, McEwen, Mogilner, Khodjakov: "Adaptive changes in the kinetochore architecture facilitate proper spindle assembly." in: Nature cell biology, Vol. 17, Issue 9, pp. 1134-44, 2015 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Shibata, Moiani, Arvai, Perry, Harding, Genois, Maity, van Rossum-Fikkert, Kertokalio, Romoli, Ismail, Ismalaj, Petricci, Neale, Bristow, Masson, Wyman, Jeggo, Tainer: "DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities." in: Molecular cell, Vol. 53, Issue 1, pp. 7-18, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Gómez-Herreros, Schuurs-Hoeijmakers, McCormack, Greally, Rulten, Romero-Granados, Counihan, Chaila, Conroy, Ennis, Delanty, Cortés-Ledesma, de Brouwer, Cavalleri, El-Khamisy, de Vries, Caldecott: "TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function." in: Nature genetics, Vol. 46, Issue 5, pp. 516-21, 2014 (PubMed).

Mannino, Gomez-Roman, Hochegger, Chalmers: "Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors: implications for stem cell mitosis and centrosome dynamics." in: Stem cell research, Vol. 13, Issue 1, pp. 135-43, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Averbeck, Ringel, Herrlitz, Jakob, Durante, Taucher-Scholz: "DNA end resection is needed for the repair of complex lesions in G1-phase human cells." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 16, pp. 2509-16, 2014 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Grundy, Rulten, Zeng, Arribas-Bosacoma, Iles, Manley, Oliver, Caldecott: "APLF promotes the assembly and activity of non-homologous end joining protein complexes." in: The EMBO journal, Vol. 32, Issue 1, pp. 112-25, 2013 (PubMed).

Bee, Fabris, Cherubini, Mognato, Celotti: "The efficiency of homologous recombination and non-homologous end joining systems in repairing double-strand breaks during cell cycle progression." in: PLoS ONE, Vol. 8, Issue 7, pp. e69061, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Rich, Henderson, Becker, Cornell, Patterson-Kane: "Indicators of replicative damage in equine tendon fibroblast monolayers." in: BMC veterinary research, Vol. 9, pp. 180, 2013 (PubMed). (Sample species: Horse (Equine)). Further details: Immunocytochemistry,Immunofluorescence

Yajima, Fujisawa, Nakajima, Hirakawa, Jeggo, Okayasu, Fujimori: "The complexity of DNA double strand breaks is a critical factor enhancing end-resection." in: DNA repair, Vol. 12, Issue 11, pp. 936-46, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Lee, Lin, Equilibrina, Uchiyama, Matsunaga, Fukui: "ASURA (PHB2) Is Required for Kinetochore Assembly and Subsequent Chromosome Congression." in: Acta histochemica et cytochemica, Vol. 44, Issue 6, pp. 247-58, 2012 (PubMed).