Centromere Protein F (CENPF) (C-Term) antibody

Details for Product No. ABIN152930
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Synonyms LOC100222985, 6530404A22Rik, AI325968, CENF, Lek1, PRO1779, mitosin, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, hcp-1, CMF1
(20), (6), (2), (2), (1), (1)
(56), (2), (1), (1)
(28), (17), (13)
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(22), (18), (17), (16), (11), (9), (8), (5), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 50 μL
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Catalog No. ABIN152930
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Immunogen A bacterial fusion protein from the C-terminus of human CENPF.
Purification Affinity purified
Alternative Name CENP-F
Background The kinetophore is a macromolecular structure that is associated with the centromeresof chromosomes and is responsible for establishing and maintaining the connectionswith the microtubules of the mitotic spindle. The proteins that reside within thechromosomal domain fall into two broad classes: those that associate with thecentromere throughout the cell cycle and those that are found at the centromere in a cellcycle specific manner. Members of the first class include CENP-A, CENP-B, andCENP-C. The members of the second class include several ubiquitous cellular proteinssuch as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochorecomplex. CENP-F also shows a cell-cycle distribution that is both temporally regulated anddiverse in terms of the structural components it localizes to and therefore may play arole in several mitotic events. (1-3). Alternate Names: anti-AH Antigen antibody, anti-CENF antibody, anti-CENP F Kinetochore Proteinantibody, anti-Centromere Protein F antibody, anti-Mitosin antibody, anti-CENPFantibody.
Gene Symbol: CENPF
Gene ID 1063
UniProt P49454
Application Notes This CENPF antibody is useful for Flow Cytometry, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Western Blot where a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.
Recommended dilutions: Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:200-1:1000, immunoprecipitation 1:50, Western Blot 1:1500-1:2500
Protocol Protocol specific for CENPF Antibody Western Blot Procedure1) Resolve aliquots (30 mg) of total HeLa cell lysate on a 8.5 % lo-crosslinker gel (110:1, acryl:bis) for efficient separation and transfer. 2) Transfer to nitrocellulose membranes in 20 mM Tris-HCL (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol. Transfer time for a 1 mm thick gel is 1 hour 15 minutes at 450mA. 3) Block membranes for 1 hour with 5 % (vol/vol) nonfat dry milk/TBS-T (20 mM Tris-HCL, pH 7.6/ 137 mM NaCl/0.1 % TWEEN 20)4) Incubate membranes for 1 hour at room temperature (RT) in NB 500-101 diluted 1:3000 in nonfat dry milk/TBS-T. 5) Wash 3X30 minutes at RT with TBS-T. 6) Incubate membranes with alkaline phosphatase conjugated anti-rabbit IgG for 1 hour (RT).
7) Wash 3 x 30 minutes at RT with TBS-T8) Develop with ECL reagents (Amersham) and autoradiography.
Restrictions For Research Use only
Format Liquid
Concentration 1.25 mg/mL
Buffer PBS with 50 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Centromere Protein F (CENPF) (C-Term) antibody Detection of CENP-F using ABIN152930 (lot D).
General Steensgaard, Garrè, Muradore et al.: "Sgt1 is required for human kinetochore assembly." in: EMBO reports, Vol. 5, Issue 6, pp. 626-31, 2004 (PubMed).

Rattner, Rao, Fritzler et al.: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, 1994 (PubMed).

Liao, Winkfein, Mack et al.: "CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis." in: The Journal of cell biology, Vol. 130, Issue 3, pp. 507-18, 1995 (PubMed).

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