Centromere Protein F (CENPF) antibody

Details for Product No. ABIN152930
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Antigen
Synonyms CENF, hcp-1, PRO1779, CMF1, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, Lek1, mitosin, AI325968, 6530404A22Rik, LOC100222985
Reactivity
Human
(39), (1), (1), (1)
Host
Rabbit
(23), (11), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(21), (13), (12), (10), (9), (9), (7), (3), (2), (2), (1)
Pubmed 3 references available
Catalog no. ABIN152930
Quantity 50 µL
Price
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Immunogen A bacterial fusion protein from the C-terminus of CENP-F.
Specificity This antibody is specific for CENP-F. Species Reactivity: This antibody is reactive against human. It has not yet been tested in other species. Localization: As cells progress through S-phase, weak nuclear staining is observed. In G2 phase,very bright CENP-F staining is detected uniformly throughout the nucleus but excludedfrom the nucleoli.
Cross-Reactivity Human
Purification Purified
Alternative Name CENP-F
Background The kinetophore is a macromolecular structure that is associated with the centromeresof chromosomes and is responsible for establishing and maintaining the connectionswith the microtubules of the mitotic spindle. The proteins that reside within thechromosomal domain fall into two broad classes: those that associate with thecentromere throughout the cell cycle and those that are found at the centromere in a cellcycle specific manner. Members of the first class include CENP-A, CENP-B, andCENP-C. The members of the second class include several ubiquitous cellular proteinssuch as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochorecomplex. CENP-F also shows a cell-cycle distribution that is both temporally regulated anddiverse in terms of the structural components it localizes to and therefore may play arole in several mitotic events. (1-3). Alternate Names: anti-AH Antigen antibody, anti-CENF antibody, anti-CENP F Kinetochore Proteinantibody, anti-Centromere Protein F antibody, anti-Mitosin antibody, anti-CENPFantibody.
Gene ID 1063
UniProt P49454
Application Notes Suggested working dilutions: immunofluorescence 1:750,immunoprecipitation 1:50,Western Blot 1:1500
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/ml
Buffer PBS, 50% glycerol and 2 mg/ml BSA. Preservative: 0.05% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Centromere Protein F (CENPF) antibody Detection of CENP-F using ABIN152930 (lot D).
General Liao, Winkfein, Mack et al.: "CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis." in: The Journal of cell biology, Vol. 130, Issue 3, pp. 507-18, 1995 (PubMed).

Rattner, Rao, Fritzler et al.: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, 1994 (PubMed).

Steensgaard, Garrue, Muradore et al.: "Sgt1 is required for human kinetochore assembly." in: EMBO reports, Vol. 5, Issue 6, pp. 626-31, 2004 (PubMed).

Hosts (23), (11), (6)
Reactivities (39), (1), (1), (1)
Applications (21), (13), (12), (10), (9), (9), (7), (3), (2), (2), (1)
Conjugates (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (3)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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