Centromere Protein F (CENPF) antibody

Antigen: Centromere Protein F (CENPF)

Synonyms: CENF, hcp-1, PRO1779, Lek1, mitosin, AI325968, 6530404A22Rik, CMF1, im:7154719, im:7156761, si:ch211-152h14.2, LOC100222985

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN152930

Additional Information

Alternative name: CENP-F

Gene ID: 1063

UniProt: P49454

Immunogen: A bacterial fusion protein from the C-terminus of CENP-F.

Cross-Reactivity: Human

Description: The kinetophore is a macromolecular structure that is associated with the centromeresof chromosomes and is responsible for establishing and maintaining the connectionswith the microtubules of the mitotic spindle. The proteins that reside within thechromosomal domain fall into two broad classes: those that associate with thecentromere throughout the cell cycle and those that are found at the centromere in a cellcycle specific manner. Members of the first class include CENP-A, CENP-B, andCENP-C. The members of the second class include several ubiquitous cellular proteinssuch as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochorecomplex. CENP-F also shows a cell-cycle distribution that is both temporally regulated anddiverse in terms of the structural components it localizes to and therefore may play arole in several mitotic events. (1-3). Alternate Names: anti-AH Antigen antibody, anti-CENF antibody, anti-CENP F Kinetochore Proteinantibody, anti-Centromere Protein F antibody, anti-Mitosin antibody, anti-CENPFantibody.

Specificity: This antibody is specific for CENP-F. Species Reactivity: This antibody is reactive against human. It has not yet been tested in other species. Localization: As cells progress through S-phase, weak nuclear staining is observed. In G2 phase,very bright CENP-F staining is detected uniformly throughout the nucleus but excludedfrom the nucleoli.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1:750,immunoprecipitation 1:50,Western Blot 1:1500

Concentration: 2.5 mg/ml

Purification: Purified

Buffer: PBS, 50% glycerol and 2 mg/ml BSA. Preservative: 0.05% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Detection of CENP-F using ABIN152930 (lot D).

Publications

Liao, Winkfein, Mack et al.: "CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis." in: The Journal of cell biology, Vol. 130, Issue 3, pp. 507-18, 1995 (PubMed).

Rattner, Rao, Fritzler et al.: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, 1994 (PubMed).

Steensgaard, Garrue, Muradore et al.: "Sgt1 is required for human kinetochore assembly." in: EMBO reports, Vol. 5, Issue 6, pp. 626-31, 2004 (PubMed).