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Asymmetrical Dimethylarginine (ADMA) antibody

Antigen

Asymmetrical Dimethylarginine (ADMA)
Clonality Monoclonal (21C7)
Host
Alternatives

Mouse
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
2 references available
Catalog no. ABIN152942
Quantity 0.2 ml
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives back to top

Alternative name Asymetric Dimethylarginine (ADMA)
Immunogen Asymmetric NG-NG-dimethyl arginine (two methyl groups onone of the terminal nitrogen atoms of the guanidinium group).
Format Ascites
Isotype IgM
Clone 21C7
Description This antibody will be of use in chromatin and transcription factor research to monitormethylation status. Since it is specific to dimethyl arginine it can be used in parallel withab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethylarginine only) to monitor the exact modification status.
Specificity This antibody reacts with free and bound asymmetricNG-NG-dimethyl arginine. It is not known whether theantibody reacts with symmetric dimethyl arginine. Species Reactivity: All Species.

Application Details
Publications Alternatives back to top

Application Notes ChIP: Use 5ul for 4x106 cells.IHC: Use at a dilution of 1:100 - 1:1000.IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:50 - 1:500. Optimal dilutions/concentrations should be determinedby the end user.
Purification Ascites
Buffer Preservative: None Constituents: Raw Ascites. Preservative: None Constituents: Raw Ascites.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Britten, Howarth, Roche: "Immunohistochemistry on resin sections: a comparison of resin embedding techniques for small mucosal biopsies." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 68, Issue 5, pp. 271-80, 1994 (PubMed).

Mowen, Tang, Zhu et al.: "Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription." in: Cell, Vol. 104, Issue 5, pp. 731-41, 2001 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Asymmetrical Dimethylarginine (ADMA)", type "Antibodies"
Hosts Rabbit (7)
Reactivities All Species (3)
Applications ELISA (7), Western Blotting (WB) (7), Immunocytochemistry (ICC) (4), Immunofluorescence (IF) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (IHC) (3)
Conjugates Biotin (1)