Asymmetrical Dimethylarginine (ADMA) antibody

Details for Product No. ABIN152942
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Antigen
Reactivity
Chemical
(4), (4)
Host
Mouse
(8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(8), (6), (6), (4), (4), (4), (4), (4), (2), (2)
Pubmed 2 references available
Quantity 0.2 mL
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Catalog No. ABIN152942
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Immunogen Asymmetric NG-dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)
Clone 21C7
Isotype IgM kappa
Specificity This reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the reacts with symmetric dimethyl arginine.
Purification Protein A purified
Alternative Name Asymetric Dimethylarginine (ADMA)
Target Type Chemical
Background This antibody will be of use in chromatin and transcription factor research to monitormethylation status. Since it is specific to dimethyl arginine it can be used in parallel withab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethylarginine only) to monitor the exact modification status.
Application Notes Recommended dilutions: Western Blot 1:500-1:5000, Immunohistochemistry-Paraffin 1:100-1:10000, Immunohistochemistry
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 200 µL of distilled water.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Britten, Howarth, Roche: "Immunohistochemistry on resin sections: a comparison of resin embedding techniques for small mucosal biopsies." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 68, Issue 5, pp. 271-80, 1994 (PubMed).

Mowen, Tang, Zhu et al.: "Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription." in: Cell, Vol. 104, Issue 5, pp. 731-41, 2001 (PubMed).

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