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Details for Product No. ABIN152942

Asymmetrical Dimethylarginine (ADMA) antibody

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Antigen
Reactivity
»Alternatives Chemical
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN152942
Quantity 0.2 mL
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Immunogen Asymmetric NG-NG-dimethyl arginine (two methyl groups onone of the terminal nitrogen atoms of the guanidinium group).
Clone 21C7
Isotype IgM
Specificity This antibody reacts with free and bound asymmetricNG-NG-dimethyl arginine. It is not known whether theantibody reacts with symmetric dimethyl arginine. Species Reactivity: All Species.
Purification Ascites
Alternative Name Asymetric Dimethylarginine (ADMA)
Background This antibody will be of use in chromatin and transcription factor research to monitormethylation status. Since it is specific to dimethyl arginine it can be used in parallel withab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethylarginine only) to monitor the exact modification status.
Application Notes ChIP: Use 5ul for 4x106 cells.IHC: Use at a dilution of 1:100 - 1:1000.IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:50 - 1:500. Optimal dilutions/concentrations should be determinedby the end user.
Restrictions For Research Use only
Format Liquid
Buffer Preservative: None Constituents: Raw Ascites. Preservative: None Constituents: Raw Ascites.
Storage 4 °C
Britten, Howarth, Roche: "Immunohistochemistry on resin sections: a comparison of resin embedding techniques for small mucosal biopsies." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 68, Issue 5, pp. 271-80, 1994 (PubMed).

Mowen, Tang, Zhu et al.: "Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription." in: Cell, Vol. 104, Issue 5, pp. 731-41, 2001 (PubMed).

Alternatives for antigen "Asymmetrical Dimethylarginine (ADMA)", type "Antibodies"
Hosts (8)
Reactivities (4), (4)
Applications (8), (8), (6), (6), (4), (4), (4), (2), (2)
Conjugates (2)
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