Histone Deacetylase 3 (HDAC3) antibody

Antigen: Histone Deacetylase 3 (HDAC3)

Synonyms: HD3, RPD3, RPD3-2, AW537363, DHDAC3, DmHDAC3, HDAC, HDAC3, dmHDA402, DmelCG2128, CG2128, MGC55927, zgc:55927, hdac3, HDm, hd3, XRpd3, rpd3-2, DKFZp469I1039

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN152952

Additional Information

Alternative name: HDAC3

Gene ID: 8841, 15183, 84578

Immunogen: Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding toamino acids 411-428 of human and chicken HDAC3.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Histones play a critical role in transcriptional regulation, cell cycle progression, anddevelopmental events. Histone cetylation/deacetylation alters chromosome structureand affects transcription factor access to DNA. The protein encoded by this genebelongs to the histone acetylase/acuc/apha family. It has histone deacetylase activityand represses transcription when tethered to a promoter. It may participate in theregulation of transcription through its binding with the zinc-finger transcription factorYY1. This protein can also down-regulate p53 function and thus modulate cell growthand apoptosis. This gene is regarded as a potential tumor suppressor gene. Alternate Names: Anti-RPD3 2 antibody, Anti-HD 3 antibody, Anti-HD3 antibody, Anti-HDAC 3 antibody,Anti-Histone deacetylase 3 (HD3) (RPD3-2) antibody, Anti-histone deacetylase 3antibody, Anti-RPD 3 antibody, Anti-RPD3 antibody, Anti-RPD3-2 antibody, Anti-SMAP45 antibody, Anti-SMAP45 antibody.

Specificity: Anti-Histone Deacetylase 3 specifically recognizes histone deacetylase 3 byimmunoblotting and immunoprecipitation (50 kD). Species Reactivity: Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: chromatin immunoprecipitation 2 ul for106cells,immunoprecipitation 1/2500,Immunohistochemistry-Paraffin 1/500. Positive Controls: HeLa, NIH3T3, and human lymph node

Purification: Purified

Buffer: 0.01M PBS [pH7.4]

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Cancer, Hypertrophy, DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Publications

Wolffe: "Histone deacetylase: a regulator of transcription." in: Science (New York, N.Y.), Vol. 272, Issue 5260, pp. 371-2, 1996 (PubMed).

Taunton, Hassig, Schreiber: "A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p." in: Science (New York, N.Y.), Vol. 272, Issue 5260, pp. 408-11, 1996 (PubMed).

Grunstein: "Histone acetylation in chromatin structure and transcription." in: Nature, Vol. 389, Issue 6649, pp. 349-52, 1997 (PubMed).

Lin, Nagy, Inoue et al.: "Role of the histone deacetylase complex in acute promyelocytic leukaemia." in: Nature, Vol. 391, Issue 6669, pp. 811-4, 1998 (PubMed).

Zhang, LeRoy, Seelig et al.: "The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities." in: Cell, Vol. 95, Issue 2, pp. 279-89, 1998 (PubMed).

Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).

Fischle, Emiliani, Hendzel et al.: "A new family of human histone deacetylases related to Saccharomyces cerevisiae HDA1p." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 11713-20, 1999 (PubMed).

Grozinger, Hassig, Schreiber: "Three proteins define a class of human histone deacetylases related to yeast Hda1p." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 9, pp. 4868-73, 1999 (PubMed).

Glaser, Li, Staver et al.: "Role of class I and class II histone deacetylases in carcinoma cells using siRNA." in: Biochemical and biophysical research communications, Vol. 310, Issue 2, pp. 529-36, 2003 (PubMed).

Li, Staver, Curtin et al.: "Expression and functional characterization of recombinant human HDAC1 and HDAC3." in: Life sciences, Vol. 74, Issue 22, pp. 2693-705, 2004 (PubMed).

Furuya, Guigon, Zhao et al.: "Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling." in: Molecular and cellular biology, Vol. 27, Issue 17, pp. 6116-26, 2007 (PubMed).