Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Ser152), (Ser156) antibody

Details for Product No. ABIN152966
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Antigen
Synonyms marcks, MGC89127, MARCKS, Macs, PKCSL, KINC, macs, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, 80K-L, MACS, PRKCSL
Epitope
Ser152, Ser156
(29), (28), (26), (26), (25), (15), (9), (8), (6), (6), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(209), (142), (131), (54), (40), (36), (25), (3), (3), (1), (1), (1)
Host
Rabbit
(211), (17), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB)
(171), (61), (58), (40), (29), (29), (12), (5), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN152966
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Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152,Ser156 conjugated to KLH
Specificity Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase
Cross-Reactivity (Details) Chinese Hamster reactivity reported in scientific literature (PMID: 17079793). Reactivity assumed based on 100% sequence identity: Bovine,Chicken, Human, Mouse, Xenopus,Zebrafish.
Purification Immunogen affinity purified
Alternative Name MARCKS
Background Myristolated Alanine-Rich C-Kinase Substrate (MARCKS) is a major in vivo substrate ofprotein kinase C (PKC) and for the lipid-activated PKC-related kinase (PRK1). Furthermore, PRK1 and OPKC phosphorylate MARCKS on the same sites in vitro,serine 152, 156, and 163. MARCKS serves as an in vitro substrate for PKCu as well. However, in contrast to other PKCs, MARCKS is phosphorylated by PKCu only at serine156 and not at serines 152 and 163, implicating a differential regulation by PKCu. Therefore, control of MARCKS phosphorylation on these previously identified 'PKC'sites may be regulated under certain circumstances by PRK as well as PKC mediatedsignaling pathways. MARCKS associates with the plasma membrane in response toPKC-catalyzed phosphorylation of MARCKS. It has been suggested that MARCKS iscapable of associating with the plasma membrane through binding to phospholipidswithout interaction with membranous proteins. Alternate Names: Anti-80K L protein antibody, Anti-80KL antibody, Anti-81 kDa protein, light chainantibody, Anti-MACS antibody, Anti-MARCS antibody, Anti-MGC52672 antibody,Anti-Myristoylated alanine rich protein kinase C substrate antibody,Anti-Phosphomyristin antibody, Anti-PKCSL antibody, Anti-PRKCSL antibody,Anti-Protein kinase C substrate antibody. Related Diseases: Cancer
Gene Symbol: MARCKS
Gene ID 4082
Application Notes The investigator should determine the optimal dilution for a specific application NB 500-140 can be used in Western blot where a band is seen at ~ 80 kDa, representing MARCKS. Immunohistochemical analysis has not yet been determined.
Recommended dilutions: Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Buffer 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50 % glycerol.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Ser152), (Ser156) antibody anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Ser152), (Ser156) antibody
General Zhang, Ahmed, McFarlane et al.: "Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways." in: Journal of lipid research, Vol. 48, Issue 2, pp. 405-16, 2007 (PubMed).

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