Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (Thr386) antibody

Details for Product No. ABIN152969
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Antigen
Synonyms
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN  ... show more
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN KINASE KINASE, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, NMAPKK, MEK show less
Epitope
Thr386
(32), (27), (26), (25), (24), (22), (21), (20), (19), (12), (12), (11), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(408), (222), (212), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(378), (41), (2), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (1), (1)
Application
Western Blotting (WB)
(329), (123), (104), (92), (60), (50), (21), (20), (14), (3), (2), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN152969
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Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH
Specificity Specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Cross-Reactivity (Details) Reactivity assumed based on 100% sequence identity: Bovine, Canine, Chicken, Human, Mouse, Primate,Xenopus.
Purification Affinity purified
Alternative Name MAP2K1
Background MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAPkinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al,2003). This pathway also plays a key role in synaptic plasticity in brain (Adams andSweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both athreonine and a tyrosine residue on MAP kinase (Kyriakis et al, 1991, Seger et al,1991, Crews et al, 1992) Conversely there also appears to be a feedbackphosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylatedby MAP kinase are Thr292 and Thr386 (Mansour et al, 1994). Alternate Names: anti-MEK1 antibody.
Gene Symbol: MAP2K1
Gene ID 5604
Application Notes NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application.
Recommended dilutions: Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES [pH 7.5], 150 mM NaCl, 0.1 mg/ml BSA and 50 % glycerol.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (Thr386) antibody Western Blots. Western Blot of recombinant WT and mutant MEK 1 immunolabeled with the Anti Thr386 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T386A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.
General Mansour, Resing, Candi et al.: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995 (PubMed).

Chong, Vikis, Guan: "Mechanisms of regulating the Raf kinase family." in: Cellular signalling, Vol. 15, Issue 5, pp. 463-9, 2003 (PubMed).

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