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MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (Thr386)

Details for Product anti-MAP2K1 Antibody No. ABIN152969, Supplier: Login to see
Antigen
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
Alternatives
anti-Rat (Rattus) MAP2K1 antibody for Western Blotting
Epitope
Thr386
44
39
33
32
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31
28
26
23
18
15
13
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13
11
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7
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3
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1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Rat (Rattus)
599
310
266
55
37
20
14
12
10
5
4
2
2
Host
Rabbit
485
122
4
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
13
13
13
10
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6
1
1
Application
Western Blotting (WB)
446
204
149
119
92
66
26
23
17
10
9
6
6
4
3
2
2
2
Supplier
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Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH
Specificity Specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Cross-Reactivity (Details) Reactivity assumed based on 100% sequence identity: Bovine, Canine, Chicken, Human, Mouse, Primate,Xenopus.
Purification Affinity purified
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAPkinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al,2003). This pathway also plays a key role in synaptic plasticity in brain (Adams andSweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both athreonine and a tyrosine residue on MAP kinase (Kyriakis et al, 1991, Seger et al,1991, Crews et al, 1992) Conversely there also appears to be a feedbackphosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylatedby MAP kinase are Thr292 and Thr386 (Mansour et al, 1994). Alternate Names: anti-MEK1 antibody.
Gene Symbol: MAP2K1
Gene ID 5604
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application.
Recommended dilutions: Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES [pH 7.5], 150 mM NaCl, 0.1 mg/ml BSA and 50 % glycerol.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (Thr386) (ABIN152969) Western Blots. Western Blot of recombinant WT and mutant MEK 1 immunolabeled with th...
Product cited in: Chong, Vikis, Guan: "Mechanisms of regulating the Raf kinase family." in: Cellular signalling, Vol. 15, Issue 5, pp. 463-9, 2003 (PubMed).

Background publications Mansour, Resing, Candi et al.: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995 (PubMed).