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MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr386)

Details for Product anti-MAP2K1 Antibody No. ABIN152969, Supplier: Log in to see
Antigen
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • Map2k1
  • MAP2K1
  • map2k1
  • MEK1
Alternatives
anti-Human MAP2K1 antibody for Western Blotting
Epitope
pThr386
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15
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1
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Reactivity
Human, Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
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Application
Simple Western (SimWes), Western Blotting (WB)
466
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78
25
22
16
10
9
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6
2
2
2
1
1
Options
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Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH
Specificity Specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Purification Immunogen affinity purified
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background Gene Symbol: MAP2K1
Gene ID 5604
UniProt Q02750
Research Area Signaling, Tyrosine Kinases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
Application Notes Western Blot 1:1000, Simple Western 1:50NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-All.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 0.15M NaCl, 0.1 mg/mL BSA and 50 % Glycerol
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr386) (ABIN152969) Western Blots. Western Blot of recombinant WT and mutant MEK 1 immunolabeled with th...
Product cited in: Chong, Vikis, Guan: "Mechanisms of regulating the Raf kinase family." in: Cellular signalling, Vol. 15, Issue 5, pp. 463-9, 2003 (PubMed).

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002 (PubMed).

Mansour, Resing, Candi, Hermann, Gloor, Herskind, Wartmann, Davis, Ahn: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995 (PubMed).

Ahn: "The MAP kinase cascade. Discovery of a new signal transduction pathway." in: Molecular and cellular biochemistry, Vol. 127-128, pp. 201-9, 1994 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Kyriakis, Brautigan, Ingebritsen, Avruch: "pp54 microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10043-6, 1991 (PubMed).

Seger, Ahn, Boulton, Yancopoulos, Panayotatos, Radziejewska, Ericsson, Bratlien, Cobb, Krebs: "Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 14, pp. 6142-6, 1991 (PubMed).