Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody

Details for Product No. ABIN152969
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Antigen
Synonyms CFC3, MAPKK1, MEK1, MKK1, PRKMK1, Mek1, MEKK1, Prkmk1, MEK, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, mek-2
Epitope
pThr386
(31), (26), (24), (22), (21), (18), (17), (15), (14), (12), (12), (11), (8), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(376), (220), (212), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1)
Host
Rabbit
(346), (43), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application
Western Blotting (WB)
(306), (110), (95), (86), (60), (44), (18), (15), (14), (3), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN152969
Quantity 0.1 mL
Price
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Immunogen ABIN152969 was raised against a Synthetic phosphopeptide corresponding to aminoacids residues surrounding the phospho Thr386 of human MEK 1.
Specificity Specific for the ~45k MEK 1 protein phosphorylated at Thr386 in Western blots ofhuman brain extracts.Phosphorylated:Thr386. Species Reactivity: ABIN152969 reacts with human.
Cross-Reactivity Human, Rat (Rattus)
Purification Affinity Purified
Alternative Name MAP2K1
Background MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAPkinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al.,2003). This pathway also plays a key role in synaptic plasticity in brain (Adams andSweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both athreonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al.,1991, Crews et al., 1992) Conversely there also appears to be a feedbackphosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylatedby MAP kinase are Thr292 and Thr386 (Mansour et al., 1994). Alternate Names: anti-MEK1 antibody.
Gene ID 5604
Application Notes Suggested working dilutions: Western Blot 1:1,000
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol
Storage -20 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody Western Blots. Western Blot of recombinant WT and mutant MEK 1 immunolabeled with the Anti Thr386 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T386A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.
General Mansour, Resing, Candi et al.: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995 (PubMed).

Chong, Vikis, Guan: "Mechanisms of regulating the Raf kinase family." in: Cellular signalling, Vol. 15, Issue 5, pp. 463-9, 2003 (PubMed).

Hosts (346), (43), (2)
Reactivities (376), (220), (212), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1)
Applications (306), (110), (95), (86), (60), (44), (18), (15), (14), (3), (1), (1), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Epitopes (31), (26), (24), (22), (21), (18), (17), (15), (14), (12), (12), (11), (8), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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