Bromodeoxyuridine (BrDU) antibody

Antigen: Bromodeoxyuridine (BrDU)

Clonality: Monoclonal (BU1-75)

Host: Rat

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN152983

Additional Information

Isotype: IgG2a

Clone: BU1-75

Description: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNAis a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or invivo labeling of cells with the thymidine analogue BrdU and the subsequent detection ofincorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate andcomprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thusprovide an estimate for the fraction of cells in S-phase. Also dynamic proliferativeinformation (such as the S-phase transit rate and the potential doubling time) can beobtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Specificity: This antibody reacts with BrdU in single stranded DNA, BrdU attached to a proteincarrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdUincorporated into their DNA. Also reacts with chlorodeoxyuridine but with reducedstaining. The antibody does not react with thymidine. The antibody does not cross reactwith IdU. Species Reactivity: All Species. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: flow cytometry / FACS analysis 1:25 - 1:200,Immunohistochemistry-Frozen 1:25 - 1:200

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS with 25% Glycerol. Preservative: 0.09% Sodium Azide.

Storage: Store at 4 degrees C short term or -20 degrees C long term.

Research Area: Proliferation Markers

Restrictions: For Research Use only

Publications

Barbie, Kudlow, Frock et al.: "Nuclear reorganization of mammalian DNA synthesis prior to cell cycle exit." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 595-607, 2003 (PubMed).

Siegenthaler, Tremper-Wells, Miller: "Foxg1 haploinsufficiency reduces the population of cortical intermediate progenitor cells: effect of increased p21 expression." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 8, pp. 1865-75, 2008 (PubMed).

Denis-Donini, Dellarole, Crociara et al.: "Impaired adult neurogenesis associated with short-term memory defects in NF-kappaB p50-deficient mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 15, pp. 3911-9, 2008 (PubMed).

Jiao, Feldheim, Chen: "Ephrins as negative regulators of adult neurogenesis in diverse regions of the central nervous system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 25, pp. 8778-83, 2008 (PubMed).

Chang, Gaysinskaya, Karatayev et al.: "Maternal high-fat diet and fetal programming: increased proliferation of hypothalamic peptide-producing neurons that increase risk for overeating and obesity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 46, pp. 12107-19, 2008 (PubMed).