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KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region)

Details for Product anti-MKI67 Antibody No. ABIN152984, Supplier: Log in to see
Antigen
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • antigen identified by monoclonal antibody Ki-67
  • antigen identified by monoclonal antibody Ki 67
  • MKI67
  • mki67
  • Mki67
Alternatives
anti-Human KI-67 antibody for Immunohistochemistry (Frozen Sections)
Epitope
Internal Region, AA 1550-1700
88
54
52
18
17
15
12
12
9
5
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
425
106
70
17
14
7
4
1
Host
Rabbit
239
148
32
11
3
Clonality
Polyclonal
Conjugate
This KI-67 antibody is un-conjugated
16
10
10
10
10
7
7
6
5
4
4
4
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
168
168
148
111
109
97
81
66
10
10
9
9
6
3
3
2
1
1
1
1
Options
Supplier
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Immunogen A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]
Isotype IgG
Purification Immunogen affinity purified
Alternative Name Ki-67/MKI67 (MKI67 Antibody Abstract)
Background Gene Symbol: MKI67
Gene ID 4288
UniProt P46013, Q91VE6
Pathways Glycosaminoglycan Metabolic Process
Application Notes Western Blot 1-2 μg/mL, Immunohistochemistry 1:50-1:300, Immunocytochemistry/Immunofluorescence 1:20-1:100, Immunohistochemistry-Paraffin 1:50-1:300, Immunohistochemistry-Frozen 1:50-1:300This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot, however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) FFPE human tonsil
Western Blotting (WB) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Western Blot: Ki67 Antibody [ABIN152984] - Western blot analysis of A431 (A) and Hek2...
Immunofluorescence (IF) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN152984] - Confocal immunof...
Immunofluorescence (IF) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN152984] - Ki67 antibody (1...
Immunofluorescence (IF) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN152984] - Ki67 antibody wa...
Product cited in: Röck, Joosse, Müller, Heinisch, Fuchs, Meusch, Zipper, Reifenberger, Pantel, Fischer: "Chronic UVB-irradiation actuates perpetuated dermal matrix remodeling in female mice: Protective role of estrogen." in: Scientific reports, Vol. 6, pp. 30482, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Xiong, Zhou, Wang, Chen, Xu, Wang, Deng, Liu: "Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice." in: Scientific reports, Vol. 6, pp. 34079, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Maeda, Chen, Bu, Mujcic, Wouters, DaCosta: "In Vivo Imaging Reveals Significant Tumor Vascular Dysfunction and Increased Tumor Hypoxia-Inducible Factor-1α Expression Induced by High Single-Dose Irradiation in a Pancreatic Tumor Model." in: International journal of radiation oncology, biology, physics, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Zimmermann, Thormann, Hu, Köhler, Imai-Matsushima, Locht, Arnett, Schlesinger, Zoller, Schürmann, Kaufmann, Wardemann: "Human isotype-dependent inhibitory antibody responses against Mycobacterium tuberculosis." in: EMBO molecular medicine, Vol. 8, Issue 11, pp. 1325-1339, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Lohse, Rasowski, Cao, Pintilie, Do, Tsao, Hill, Hedley: "Targeting hypoxic microenvironment of pancreatic xenografts with the hypoxia-activated prodrug TH-302." in: Oncotarget, Vol. 7, Issue 23, pp. 33571-80, 2016 (PubMed). (Sample species: Mouse (Murine)).

Kawanishi, Nakasa, Shoji, Hamanishi, Shimizu, Kamei, Usman, Ochi: "Intra-articular injection of synthetic microRNA-210 accelerates avascular meniscal healing in rat medial meniscal injured model." in: Arthritis research & therapy, Vol. 16, Issue 6, pp. 488, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Nakagawa, Muneta, Kondo, Mizuno, Takakuda, Ichinose, Tabuchi, Koga, Tsuji, Sekiya: "Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs." in: Osteoarthritis and cartilage, Vol. 23, Issue 6, pp. 1007-17, 2015 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Spiegelberg, Braks, Djasim, Farrell, van der Wal, Wolvius: "Effects of hyperbaric oxygen therapy on the viability of irradiated soft head and neck tissues in mice." in: Oral diseases, Vol. 20, Issue 3, pp. e111-9, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Saggar, Tannock: "Activity of the hypoxia-activated pro-drug TH-302 in hypoxic and perivascular regions of solid tumors and its potential to enhance therapeutic effects of chemotherapy." in: International journal of cancer, Vol. 134, Issue 11, pp. 2726-34, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Gou, Huang, Shi, Chen, Wang: "Chemo-immunotherapy with oxaliplatin and interleukin-7 inhibits colon cancer metastasis in mice." in: PLoS ONE, Vol. 9, Issue 1, pp. e85789, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Mertens, Hermann, Bühren, Olde-Damink, Möckel, Gremse, Ehling, Kiessling, Lammers: "Iron Oxide-labeled Collagen Scaffolds for Non-invasive MR Imaging in Tissue Engineering." in: Advanced functional materials, Vol. 24, Issue 6, pp. 754-762, 2014 (PubMed).

Kwon, Kim, Lee, Sohn, Park: "A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 6, Issue 4, pp. 430-7, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Sherman-Baust, Kuhn, Valle, Shih, Kurman, Wang, Amano, Ko, Miyoshi, Araki, Lehrmann, Zhang, Becker, Morin: "A genetically engineered ovarian cancer mouse model based on fallopian tube transformation mimics human high-grade serous carcinoma development." in: The Journal of pathology, Vol. 233, Issue 3, pp. 228-37, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Smith, Matheson, Trombly, Sun, Campeau, Han, Yates, Kaufman: "A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli." in: Molecular biology of the cell, Vol. 25, Issue 18, pp. 2866-81, 2014 (PubMed). (Sample species: Human).

Xie, Cui, Wang, Xia, Hu, Xian, Li, Xie, Crane, Wan, Zhen, Bian, Yu, Chang, Qiu, Pickarski, Duong, Windle, Luo, Liao, Cao: "PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis." in: Nature medicine, Vol. 20, Issue 11, pp. 1270-8, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Uegaki, Taniguchi, Nakamura, Osaki, Okada, Yamamoto, Harada: "Inhibitor of apoptosis proteins (IAPs) may be effective therapeutic targets for treating endometriosis." in: Human reproduction (Oxford, England), Vol. 30, Issue 1, pp. 149-58, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Xiang, Gilkes, Chaturvedi, Luo, Hu, Takano, Liang, Semenza: "Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 2, pp. 151-64, 2014 (PubMed).

Sano, Sasaki, Hirakawa, Sakabe, Ogawa, Baba, Zaima, Tanaka, Inuzuka, Yamamoto, Setou, Sato, Konno, Unno: "Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm." in: PLoS ONE, Vol. 9, Issue 3, pp. e89830, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Takano, Sarfraz, Gilkes, Chaturvedi, Xiang, Suematsu, Zagzag, Semenza: "Decreased expression of cystathionine ?-synthase promotes glioma tumorigenesis." in: Molecular cancer research : MCR, Vol. 12, Issue 10, pp. 1398-406, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Hu, Takano, Xiang, Gilkes, Luo, Semenza: "Hypoxia-inducible factors enhance glutamate signaling in cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 8853-68, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)