KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region)

Details for Product anti-MKI67 Antibody No. ABIN152984, Supplier: Log in to see
Antigen
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • antigen identified by monoclonal antibody Ki-67
  • antigen identified by monoclonal antibody Ki 67
  • MKI67
  • mki67
  • Mki67
Alternatives
anti-Rat (Rattus) KI-67 antibody for BioImaging
Epitope
Internal Region, AA 1550-1700
90
54
51
18
18
15
15
12
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
456
111
71
17
14
9
4
2
1
Host
Rabbit
258
158
32
10
5
3
Clonality
Polyclonal
Conjugate
This KI-67 antibody is un-conjugated
17
13
11
10
10
8
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
186
180
156
120
117
102
84
71
16
13
13
11
6
3
3
2
1
1
1
1
Options
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Immunogen A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]
Isotype IgG
Purification Immunogen affinity purified
Alternative Name Ki-67/MKI67 (MKI67 Antibody Abstract)
Background Gene Symbol: MKI67
Gene ID 4288
UniProt P46013, Q91VE6
Pathways Glycosaminoglycan Metabolic Process
Application Notes Western Blot 1-2 μg/mL, Immunohistochemistry 1:50-1:300, Immunocytochemistry/Immunofluorescence 1:20-1:100, Immunohistochemistry-Paraffin 1:50-1:300, Immunohistochemistry-Frozen 1:50-1:300This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot, however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) FFPE human tonsil
Western Blotting (WB) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Western Blot: Ki67 Antibody [ABIN152984] - Western blot analysis of A431 (A) and Hek2...
Immunofluorescence (IF) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN152984] - Confocal immunof...
Immunofluorescence (IF) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN152984] - Ki67 antibody (1...
Immunofluorescence (IF) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (Internal Region) (ABIN152984) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN152984] - Ki67 antibody wa...
Product cited in: Tan, Joshua, Wang, Bristow, Wouters, Allen, Tannock: "Up-regulation of autophagy is a mechanism of resistance to chemotherapy and can be inhibited by pantoprazole to increase drug sensitivity." in: Cancer chemotherapy and pharmacology, Vol. 79, Issue 5, pp. 959-969, 2017 (PubMed). (Sample species: Human).

Röck, Joosse, Müller, Heinisch, Fuchs, Meusch, Zipper, Reifenberger, Pantel, Fischer: "Chronic UVB-irradiation actuates perpetuated dermal matrix remodeling in female mice: Protective role of estrogen." in: Scientific reports, Vol. 6, pp. 30482, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Xiong, Zhou, Wang, Chen, Xu, Wang, Deng, Liu: "Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice." in: Scientific reports, Vol. 6, pp. 34079, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Maeda, Chen, Bu, Mujcic, Wouters, DaCosta: "In Vivo Imaging Reveals Significant Tumor Vascular Dysfunction and Increased Tumor Hypoxia-Inducible Factor-1α Expression Induced by High Single-Dose Irradiation in a Pancreatic Tumor Model." in: International journal of radiation oncology, biology, physics, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Zimmermann, Thormann, Hu, Köhler, Imai-Matsushima, Locht, Arnett, Schlesinger, Zoller, Schürmann, Kaufmann, Wardemann: "Human isotype-dependent inhibitory antibody responses against Mycobacterium tuberculosis." in: EMBO molecular medicine, Vol. 8, Issue 11, pp. 1325-1339, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Lohse, Rasowski, Cao, Pintilie, Do, Tsao, Hill, Hedley: "Targeting hypoxic microenvironment of pancreatic xenografts with the hypoxia-activated prodrug TH-302." in: Oncotarget, Vol. 7, Issue 23, pp. 33571-80, 2016 (PubMed). (Sample species: Mouse (Murine)).

Huang, Lien, Kuo, Huang: "DDA suppresses angiogenesis and tumor growth of colorectal cancer in vivo through decreasing VEGFR2 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63124-63137, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Peng, Chen, Ge, Zhu, Shi, Li, Yu, Chu, Yang: "miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes." in: International journal of molecular sciences, Vol. 17, Issue 12, 2016 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC)

Jayaraman, Doucet, Kominsky: "Down-regulation of CITED2 attenuates breast tumor growth, vessel formation and TGF-β-induced expression of VEGFA." in: Oncotarget, Vol. 8, Issue 4, pp. 6169-6178, 2016 (PubMed). (Sample species: Human).

Kawanishi, Nakasa, Shoji, Hamanishi, Shimizu, Kamei, Usman, Ochi: "Intra-articular injection of synthetic microRNA-210 accelerates avascular meniscal healing in rat medial meniscal injured model." in: Arthritis research & therapy, Vol. 16, Issue 6, pp. 488, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Nakagawa, Muneta, Kondo, Mizuno, Takakuda, Ichinose, Tabuchi, Koga, Tsuji, Sekiya: "Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs." in: Osteoarthritis and cartilage, Vol. 23, Issue 6, pp. 1007-17, 2015 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

de la Puente, Muz, Gilson, Azab, Luderer, King, Achilefu, Vij, Azab: "3D tissue-engineered bone marrow as a novel model to study pathophysiology and drug resistance in multiple myeloma." in: Biomaterials, Vol. 73, pp. 70-84, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Spiegelberg, Braks, Djasim, Farrell, van der Wal, Wolvius: "Effects of hyperbaric oxygen therapy on the viability of irradiated soft head and neck tissues in mice." in: Oral diseases, Vol. 20, Issue 3, pp. e111-9, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Saggar, Tannock: "Activity of the hypoxia-activated pro-drug TH-302 in hypoxic and perivascular regions of solid tumors and its potential to enhance therapeutic effects of chemotherapy." in: International journal of cancer, Vol. 134, Issue 11, pp. 2726-34, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Gou, Huang, Shi, Chen, Wang: "Chemo-immunotherapy with oxaliplatin and interleukin-7 inhibits colon cancer metastasis in mice." in: PLoS ONE, Vol. 9, Issue 1, pp. e85789, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Mertens, Hermann, Bühren, Olde-Damink, Möckel, Gremse, Ehling, Kiessling, Lammers: "Iron Oxide-labeled Collagen Scaffolds for Non-invasive MR Imaging in Tissue Engineering." in: Advanced functional materials, Vol. 24, Issue 6, pp. 754-762, 2014 (PubMed).

Kwon, Kim, Lee, Sohn, Park: "A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 6, Issue 4, pp. 430-7, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Sherman-Baust, Kuhn, Valle, Shih, Kurman, Wang, Amano, Ko, Miyoshi, Araki, Lehrmann, Zhang, Becker, Morin: "A genetically engineered ovarian cancer mouse model based on fallopian tube transformation mimics human high-grade serous carcinoma development." in: The Journal of pathology, Vol. 233, Issue 3, pp. 228-37, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Smith, Matheson, Trombly, Sun, Campeau, Han, Yates, Kaufman: "A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli." in: Molecular biology of the cell, Vol. 25, Issue 18, pp. 2866-81, 2014 (PubMed). (Sample species: Human).

Xie, Cui, Wang, Xia, Hu, Xian, Li, Xie, Crane, Wan, Zhen, Bian, Yu, Chang, Qiu, Pickarski, Duong, Windle, Luo, Liao, Cao: "PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis." in: Nature medicine, Vol. 20, Issue 11, pp. 1270-8, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry