Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (Internal Region), (AA 1550-1700) antibody

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Antigen
Synonyms MKI67, kia, mki67, MGC132156, D630048A14Rik, Ki-67, Ki67, KIA, MIB-1
Epitope
Internal Region, AA 1550-1700
(37), (7), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(200), (30), (28), (2), (1), (1)
Host
Rabbit
(124), (76), (6), (2), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(95), (68), (62), (58), (36), (28), (17), (10), (9), (1), (1)
Pubmed 10 references available
Quantity 0.1 mL
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Catalog No. ABIN152984
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Immunogen A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700)
Isotype IgG
Purification affinity purified
Alternative Name Ki-67
Background Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed byproliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It isabsent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growingfraction in neoplasms. In neoplastic tissues the prognostic value is comparable to thetritiated thymidine-labelling index. The correlation between low Ki67 index andhistologically low-grade tumours is strong. Ki67 is routinely used as a neuronal markerof cell cycling and proliferation. Alternate Names: anti-Antigen identified by monoclonal Ki 67 antibody, anti-Antigen KI-67 antibody,anti-KIA antibody, anti-Proliferation related Ki 67 antigen antibody, anti-ki-67 antibody.
Gene Symbol: MKI67
Gene ID 4288, 17345, 291234
UniProt P46013
Research Area Proliferation Markers, Cell Cycle
Application Notes This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot, however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:20-1:100, Immunohistochemistry 1:50-1:300, Immunohistochemistry-Frozen 1:50-1:300, Immunohistochemistry-Paraffin 1:50-1:300, Western Blot 1-2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 1.03 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (Internal Region), (AA 1550-1700) antibody FFPE human tonsil
General Gerdes, Li, Schlueter et al.: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).

Knott, Juno, Jarboe et al.: "Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 287, Issue 3, pp. G562-70, 2004 (PubMed).

Zecchini, Domaschenz, Winton et al.: "Notch signaling regulates the differentiation of post-mitotic intestinal epithelial cells." in: Genes & development, Vol. 19, Issue 14, pp. 1686-91, 2005 (PubMed).

Micci, Pattillo, Kahrig et al.: "Caspase inhibition increases survival of neural stem cells in the gastrointestinal tract." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 17, Issue 4, pp. 557-64, 2005 (PubMed).

Kelly, Moeller, Juneja et al.: "The G12 family of heterotrimeric G proteins promotes breast cancer invasion and metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 21, pp. 8173-8, 2006 (PubMed).

Ip, McGee, Masso-Welch et al.: "The t10,c12 isomer of conjugated linoleic acid stimulates mammary tumorigenesis in transgenic mice over-expressing erbB2 in the mammary epithelium." in: Carcinogenesis, Vol. 28, Issue 6, pp. 1269-76, 2007 (PubMed).

Kojima, Vickers, Adsay et al.: "Inactivation of Smad4 accelerates Kras(G12D)-mediated pancreatic neoplasia." in: Cancer research, Vol. 67, Issue 17, pp. 8121-30, 2007 (PubMed).

Dai, Freudenberger, Zipper et al.: "Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1451-61, 2007 (PubMed).

Olofsson, Soederstroem, Wuagsaeter et al.: "CD137 is expressed in human atherosclerosis and promotes development of plaque inflammation in hypercholesterolemic mice." in: Circulation, Vol. 117, Issue 10, pp. 1292-301, 2008 (PubMed).

Campa, Gutiuerrez-Lanza, Cerignoli et al.: "Notch activates cell cycle reentry and progression in quiescent cardiomyocytes." in: The Journal of cell biology, Vol. 183, Issue 1, pp. 129-41, 2008 (PubMed).

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