Survivin antibody (BIRC5)

Details for Product anti-BIRC5 Antibody No. ABIN153009, Supplier: Log in to see
Antigen
  • bir5a
  • survivin
  • LOC398389
  • BIRC5
  • BIR1
  • Su2
  • Survivin
  • Survivin1
  • XBIR1
  • api4
  • epr-1
  • svv1
  • xSurvivin1
  • xsurv
  • SIX
  • Su1
  • svv2
  • birc5
  • xSurvivin
  • xSurvivin2
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • baculoviral IAP repeat-containing 5a
  • survivin
  • baculoviral IAP repeat-containing 5
  • baculoviral IAP repeat-containing 5, gene 1
  • baculoviral IAP repeat containing 5
  • birc5a
  • LOC398389
  • BIRC5
  • birc5.1
  • birc5.2
  • AaeL_AAEL012446
  • CpipJ_CPIJ001663
  • CpipJ_CPIJ002689
  • CpipJ_CPIJ006694
  • Birc5
Alternatives
anti-Rat (Rattus) Survivin antibody for Blocking Peptide
Reactivity
Cat (Feline), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus)
425
166
162
34
19
18
11
4
2
2
1
Host
Rabbit
293
139
16
1
Clonality
Polyclonal
Conjugate
This Survivin antibody is un-conjugated
24
17
16
13
13
13
12
11
9
9
8
8
6
5
5
5
5
3
3
3
2
2
2
2
2
2
2
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
346
202
179
158
147
123
106
32
32
26
7
5
3
3
3
2
2
2
2
2
1
1
Options
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Purification Immunogen affinity purified
Alternative Name Survivin (BIRC5 Antibody Abstract)
Background Gene Symbol: BIRC5
Molecular Weight Theoretical MW: 16.5 kDa
Gene ID 332
UniProt O15392
Research Area Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Pathways Nuclear Hormone Receptor Binding
Application Notes Western Blot 1 μg/mL, Simple Western 1:25, Chromatin Immunoprecipitation 1:10-1:500, Flow Cytometry, ELISA, Immunohistochemistry 1:50-1:100, Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:500, Immunohistochemistry-FrozenThis Survivin antibody is useful for Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band at approx. 16.5 kDa can be seen. For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin. Immunohistochemistry-Frozen and Flow Cytometry were reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocol Specific for Survivin Antibody Western Blot Procedure1) Cells were pelleted, washed in 1XPBS, suspended in ice water (~ 5 x 10(6) cells/ml), and placed on ice2) Lysates were prepared with the addition of 2X lysis buffer [2 % SDS/ 50mM Tris-HCl / 10 % glycerol]3) Lysates were heated to 95 degrees C for 3 minutes and then microfuged at room temperature for 10 minutes4) 50 µg of lysate were electrophoresed (150 V) through a 4-15 % PAGE5) Proteins were transferred (60 V) onto an Immobilon-P membrane (Millipore Corp.) for 45 minutes6) The blot was blocked overnight at 4 degrees C in blocking buffer [1XPBS, pH 7 / 5 % nonfat milk / 0.1 % Tween-20]7) Washed the blot in 1XPBS / 0.1 % Tween-208) Incubated the blot with 1 µg/ml of anti-Survivin antibody, diluted in blocking buffer, for 2 hours at room temperature9) Washed the blot in 1XPBS / 0.1 % Tween-2010) Reacted the blot with HRP-conjugated donkey anti-rabbit Ig, diluted in 1XPBS / 0.1 % Tween-20, for 30 minutes at room temperature11) Washed the blot in 1XPBS / 0.1 % Tween-2012) Visualized blot by ECL and autoradiographyNOTE: HeLa whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Survivin antibody (BIRC5) (ABIN153009) anti-Survivin (BIRC5) antibody
Immunofluorescence (IF) image for anti-Survivin antibody (BIRC5) (ABIN153009) Telophase with accumulation of survivin in the midbodies of two daughter cells. Survi...
Western Blotting (WB) image for anti-Survivin antibody (BIRC5) (ABIN153009) Detection of survivin in HeLa whole cell lysate (30 ug) using ABIN153009 at 1ug/ml (1...
Simple Western (SimWes) image for anti-Survivin antibody (BIRC5) (ABIN153009) Simple Western: Survivin Antibody [ABIN446306] - Simple Western lane view shows a spe...
Immunofluorescence (IF) image for anti-Survivin antibody (BIRC5) (ABIN153009) Immunocytochemistry/Immunofluorescence: Survivin Antibody [ABIN446306] - IF Confocal ...
Western Blotting (WB) image for anti-Survivin antibody (BIRC5) (ABIN153009) Western Blot: Survivin Antibody [ABIN446306] - analysis of Survivin in human hepatocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Survivin antibody (BIRC5) (ABIN153009) Immunohistochemistry-Paraffin: Survivin Antibody [ABIN446306] - IHC staining of Survi...
Immunohistochemistry (IHC) image for anti-Survivin antibody (BIRC5) (ABIN153009) Immunohistochemistry: Survivin Antibody [ABIN446306] - HRP conjugated Survivin expres...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Survivin antibody (BIRC5) (ABIN153009) Immunohistochemistry-Paraffin: Survivin Antibody [ABIN446306] - analysis of Survivin ...
Western Blotting (WB) image for anti-Survivin antibody (BIRC5) (ABIN153009) Western Blot: Survivin Antibody [ABIN446306] - Analysis of Survivin in Hela cell lysa...
Product cited in: Kim, Cheon, Kim: "Mitochondrial CCAR2/DBC1 is required for cell survival against rotenone-induced mitochondrial stress." in: Biochemical and biophysical research communications, Vol. 485, Issue 4, pp. 782-789, 2017 (PubMed).

Dheekollu, Malecka, Wiedmer, Delecluse, Chiang, Altieri, Messick, Lieberman: "Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency." in: Oncotarget, Vol. 8, Issue 5, pp. 7248-7264, 2017 (PubMed).

Liu, Shi, Yang, Ye, Wang, Dong, Guo, Liao, Song, Xu, Hu, Zhang, Deng: "Nuclear survivin promoted by acetylation is associated with the aggressive phenotype of oral squamous cell carcinoma." in: Cell cycle (Georgetown, Tex.), Vol. 16, Issue 9, pp. 894-902, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Tung, Tew, Hsu, Khung: "A novel 4-arm DNA/RNA Nanoconstruct triggering Rapid Apoptosis of Triple Negative Breast Cancer Cells within 24 hours." in: Scientific reports, Vol. 7, Issue 1, pp. 793, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Scheer, Knauer, Verhaegh: "Survivin expression pattern in the intestine of normoxic and ischemic rats." in: BMC gastroenterology, Vol. 17, Issue 1, pp. 76, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rat (Rattus)).

Magadum, Ding, He, Kim, Vasudevarao, Long, Yang, Wickramasinghe, Renikunta, Dubois, Weidinger, Yang, Engel: "Live cell screening platform identifies PPARδ as a regulator of cardiomyocyte proliferation and cardiac repair." in: Cell research, Vol. 27, Issue 8, pp. 1002-1019, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Wang, Yang, Xu, Zou, Zhou, Bian, Wang: "Survivin mRNA-circulating tumor cells are associated with prostate cancer metastasis." in: Tumour biology, Vol. 37, Issue 1, pp. 723-7, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lotti, Palazzo, Petrachi, Dallaglio, Saltari, Truzzi, Quadri, Puviani, Maiorana, Marconi, Pincelli: "Survivin Modulates Squamous Cell Carcinoma-Derived Stem-Like Cell Proliferation, Viability and Tumor Formation in Vivo." in: International journal of molecular sciences, Vol. 17, Issue 1, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Werner, Tamkan-Ölcek, Dizdar, Riemer, Wolf, Cupisti, Verde, Knoefel, Krieg: "Survivin and XIAP: two valuable biomarkers in medullary thyroid carcinoma." in: British journal of cancer, Vol. 114, Issue 4, pp. 427-34, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Wu, Tang, Marquez, Li, Highfill, He, Lian, Lin, Fuchs, Ji, Li, Xu: "Overcoming chemo/radio-resistance of pancreatic cancer by inhibiting STAT3 signaling." in: Oncotarget, Vol. 7, Issue 10, pp. 11708-23, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Iimori, Watanabe, Kiyonari, Matsuoka, Sakasai, Saeki, Oki, Kitao, Maehara: "Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability." in: Nature communications, Vol. 7, pp. 11117, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Pace, Di Vincenzo, Ferraro, Bruno, Dino, Bonsignore, Battaglia, Saibene, Lanata, Gjomarkaj: "Carbocysteine counteracts the effects of cigarette smoke on cell growth and on the SIRT1/FoxO3 axis in bronchial epithelial cells." in: Experimental gerontology, Vol. 81, pp. 119-28, 2016 (PubMed).

Genshaft, Li, Gallant, Darmanis, Prakadan, Ziegler, Lundberg, Fredriksson, Hong, Regev, Livak, Landegren, Shalek: "Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction." in: Genome biology, Vol. 17, Issue 1, pp. 188, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Tastekin, Caloglu, Puyan, Tokuc, Caloglu, Yalta, Can, Guler: "Prognostic Value of Angiogenesis and Survivin Expression in Patients with Glioblastoma." in: Turkish neurosurgery, Vol. 26, Issue 4, pp. 484-90, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Redli, Gasic, Meraldi, Nigg, Santamaria: "The Ska complex promotes Aurora B activity to ensure chromosome biorientation." in: The Journal of cell biology, Vol. 215, Issue 1, pp. 77-93, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Qi, Kudo, Tang, Liu, Jin, Liu, Zuo, Mi, Shao, Ma, Tsunematsu, Ishimaru, Zeng, Tatsuka, Shimamoto: "PARP6 acts as a tumor suppressor via downregulating Survivin expression in colorectal cancer." in: Oncotarget, Vol. 7, Issue 14, pp. 18812-24, 2016 (PubMed).

Unruhe-Knauf, Knauer: "Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization." in: Methods in molecular biology (Clifton, N.J.), Vol. 1510, pp. 47-59, 2016 (PubMed).

Varughese, Torp: "Survivin and gliomas: A literature review." in: Oncology letters, Vol. 12, Issue 3, pp. 1679-1686, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Makii, Oda, Ikeda, Sone, Hasegawa, Uehara, Nishijima, Asada, Koso, Fukuda, Inaba, Oki, Machino, Kojima, Kashiyama, Mori-Uchino, Arimoto, Wada-Hiraike, Kawana, Yano, Fujiwara, Aburatani, Osuga, Fujii: "MDM2 is a potential therapeutic target and prognostic factor for ovarian clear cell carcinomas with wild type TP53." in: Oncotarget, Vol. 7, Issue 46, pp. 75328-75338, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Alzoubi, Brody, Rahman, Mahul-Mellier, Mercado, Ito, El-Bahrawy, Silver, Boobis, Bell, Hajji: "Synergy between histone deacetylase inhibitors and DNA-damaging agents is mediated by histone deacetylase 2 in colorectal cancer." in: Oncotarget, Vol. 7, Issue 28, pp. 44505-44521, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).