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Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Details for Product No. ABIN153011
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Antigen
Synonyms API4, EPR-1, AAC-11, Api4, TIAP, survivin40, AP14
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(66), (25), (8), (1), (1), (1), (1)
Host
Mouse
(68), (14), (2)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(68), (25), (24), (17), (17), (12), (7), (4), (4), (3), (1), (1), (1)
Pubmed 9 references available
Quantity 0.1 mL
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Catalog No. ABIN153011
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Immunogen Full length recombinant human Survivin
Clone 60-11
Isotype IgG2a kappa
Specificity NB500-205 is specific for the cytoplasmic form of survivin.
Purification Ascites
Alternative Name IAP4 / BIRC5
Background Regulated inhibition of programmed cell death (apoptosis) preserves normalhomeostasis and tissue and organ morphogenesis. Aberrations in this processcontribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have beenfound in various species, including human. IAP proteins contain one/three Cys/Hisbaculovirus IAP repeats plus a C-terminal RING finger and are thought to block anevolutionary conserved step in apoptosis.Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expressionis turned off during fetal development and is not found in non-neoplastic adult humantissues. Survivin becomes abundantly re-expressed in transformed cells and in all ofthe most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division,governing a checkpoint involved in cytokinesis while also suppressing apoptosis. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) ofadult rats following traumatic brain injury. Survivin has been found co-expressed withNeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affectregulation of neural cell proliferative responses after brain injury. Alternate Names: anti-API4 antibody, anti-Apoptosis Inhibitor 4 antibody, anti-Apoptosis inhibitor survivinantibody, anti-Baculoviral IAP repeat containing 5 antibody, anti-BIRC 5 antibody,anti-EPR 1 antibody, anti-IAP4 antibody, anti-SVV antibody, anti-TIAP antibody,anti-BIRC5 antibody.
Gene Symbol: BIRC5
Gene ID 332, 11799, 64041
UniProt O15392
Research Area Cancer, Apoptosis/Necrosis, Neurology
Application Notes This Survivin (60.11) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Immunohistochemistry-Paraffin and Western Blot were reported in scientific literature. This antibody weakly detects Survivin in Western blot, for this application we suggest using NB500-235 instead. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 22841823)
Recommended dilutions: Flow Cytometry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation 1:10-1:500, Western Blot
Protocol Protocol specific for Survivin Antibody Western Blot Procedure 1) HeLa whole cell lysates were heated to 70 degrees C for 10 minutes and then microfuged at room temperature. 2) 65 mg of lysate were electrophoresed (200 V) through a 4-12 % NuPAGE Bis-Tris gel, for 1 hr. 3) Proteins were transferred (30 V) onto a nitrocellulose membrane for 40 minutes. 4) The membrane was incubated for 1 hr. at ~ 27 degrees C (RT) in blocking buffer [TBST / 5 % nonfat milk]. 5) Rinsed the membrane twice in TBS. 6) Incubated the membrane with a dilution of anti-Survivin antibody, diluted in TBS + 1 % BSA, overnight at 4degrees C, gently shaking. 7) Washed the membrane in TBST, 1x15 min, 3x5min. 8) Incubated the membrane with a goat anti-mouse secondary antibody, diluted in TBS + 1 % BSA, gently shaking for 35 minutes at room temperature. 9) Washed the membrane in TBST, 1x15 min, 3x5min. 10) Visualized membrane by ECL (AlphaInnotech ). NOTE: HeLa whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody Survivin detected in rat aorta smooth muscle cell lysate using ABIN153011.
General Fortugno, Wall, Giodini et al.: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002 (PubMed).

Allen, Florell, Hanks et al.: "Survivin expression in mouse skin prevents papilloma regression and promotes chemical-induced tumor progression." in: Cancer research, Vol. 63, Issue 3, pp. 567-72, 2003 (PubMed).

Xu, Zhao, Ding et al.: "Promyelocytic leukemia protein 4 induces apoptosis by inhibition of survivin expression." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 1838-44, 2004 (PubMed).

Ansell, Arendt, Grote et al.: "Inhibition of survivin expression suppresses the growth of aggressive non-Hodgkin's lymphoma." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 18, Issue 3, pp. 616-23, 2004 (PubMed).

Ryan, ODonovan, Browne et al.: "Expression of survivin and its splice variants survivin-2B and survivin-DeltaEx3 in breast cancer." in: British journal of cancer, Vol. 92, Issue 1, pp. 120-4, 2005 (PubMed).

Brocardo, Lei, Tighe et al.: "Mitochondrial targeting of adenomatous polyposis coli protein is stimulated by truncating cancer mutations: regulation of Bcl-2 and implications for cell survival." in: The Journal of biological chemistry, Vol. 283, Issue 9, pp. 5950-9, 2008 (PubMed).

Hino, Uozaki, Inoue et al.: "Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A." in: Cancer research, Vol. 68, Issue 5, pp. 1427-35, 2008 (PubMed).

Levkau, Schaefers, Wohlschlaeger et al.: "Survivin determines cardiac function by controlling total cardiomyocyte number." in: Circulation, Vol. 117, Issue 12, pp. 1583-93, 2008 (PubMed).

Huang, Fletcher, Beeharry et al.: "Abnormal cytokinesis after X-irradiation in tumor cells that override the G2 DNA damage checkpoint." in: Cancer research, Vol. 68, Issue 10, pp. 3724-32, 2008 (PubMed).

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