Survivin antibody (BIRC5) (Cytoplasmic Form)

Details for Product anti-BIRC5 Antibody No. ABIN153011, Supplier: Log in to see
Antigen
  • bir5a
  • survivin
  • LOC398389
  • BIRC5
  • BIR1
  • Su2
  • Survivin
  • Survivin1
  • XBIR1
  • api4
  • epr-1
  • svv1
  • xSurvivin1
  • xsurv
  • SIX
  • Su1
  • svv2
  • birc5
  • xSurvivin
  • xSurvivin2
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • baculoviral IAP repeat-containing 5a
  • survivin
  • baculoviral IAP repeat-containing 5
  • baculoviral IAP repeat-containing 5, gene 1
  • baculoviral IAP repeat containing 5
  • birc5a
  • LOC398389
  • BIRC5
  • birc5.1
  • birc5.2
  • AaeL_AAEL012446
  • CpipJ_CPIJ001663
  • CpipJ_CPIJ002689
  • CpipJ_CPIJ006694
  • Birc5
Alternatives
anti-Rat (Rattus) Survivin antibody for Blocking Peptide
Epitope
Cytoplasmic Form
65
25
23
18
18
16
15
15
13
7
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
407
166
161
35
20
19
12
4
2
2
1
Host
Mouse
294
120
16
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Survivin antibody is un-conjugated
23
16
14
12
12
12
11
10
8
8
7
7
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
332
187
166
145
132
109
91
32
32
26
7
5
3
3
3
3
2
2
2
2
1
1
1
Options
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Clone 60-11
Isotype IgG2a kappa
Specificity This antibody is specific for the cytoplasmic form of survivin.
Purification Unpurified
Alternative Name Survivin (BIRC5 Antibody Abstract)
Background Gene Symbol: BIRC5
Molecular Weight Theoretical MW: 16.5 kDa
Gene ID 332
UniProt O15392
Research Area Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Pathways Nuclear Hormone Receptor Binding
Application Notes Western Blot, Flow Cytometry 1:200, ELISA, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenThis Survivin (60.11) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Immunohistochemistry-Paraffin and Western Blot were reported in scientific literature. This antibody weakly detects Survivin in Western blot, for this application we suggest using NB500-235 instead. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 22841823) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Survivin Antibody Western Blot Procedure 1) HeLa whole cell lysates were heated to 70 degrees C for 10 minutes and then microfuged at room temperature. 2) 65 mg of lysate were electrophoresed (200 V) through a 4-12 % NuPAGE Bis-Tris gel, for 1 hr. 3) Proteins were transferred (30 V) onto a nitrocellulose membrane for 40 minutes. 4) The membrane was incubated for 1 hr. at ~ 27 degrees C (RT) in blocking buffer [TBST / 5 % nonfat milk]. 5) Rinsed the membrane twice in TBS. 6) Incubated the membrane with a dilution of anti-Survivin antibody, diluted in TBS + 1 % BSA, overnight at 4degrees C, gently shaking. 7) Washed the membrane in TBST, 1x15 min, 3x5min. 8) Incubated the membrane with a goat anti-mouse secondary antibody, diluted in TBS + 1 % BSA, gently shaking for 35 minutes at room temperature. 9) Washed the membrane in TBST, 1x15 min, 3x5min. 10) Visualized membrane by ECL (AlphaInnotech ). NOTE: HeLa whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Buffer Ascites with PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Survivin detected in rat aorta smooth muscle cell lysate using ABIN153011.
Flow Cytometry (FACS) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Flow Cytometry: Survivin Antibody (60.11) [ABIN153011] - Staining of NTERA-2 cells us...
Western Blotting (WB) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Western Blot: Survivin Antibody (60.11) [ABIN153011] - Detection in 30ug of HeLa whol...
Western Blotting (WB) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Western Blot: Survivin Antibody (60.11) [ABIN153011] - Cells were transfected with th...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [ABIN153011] - Staining of o...
Immunofluorescence (IF) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [ABIN153011] - Dete...
Immunofluorescence (IF) image for anti-Survivin antibody (BIRC5) (Cytoplasmic Form) (ABIN153011) Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [ABIN153011] - HeLa...
Product cited in: Kapinas, Kim, Mandeville, Martin-Buley, Croce, Lian, van Wijnen, Stein, Altieri, Stein: "microRNA-mediated survivin control of pluripotency." in: Journal of cellular physiology, Vol. 230, Issue 1, pp. 63-70, 2014 (PubMed). (Sample species: Human).

Hoel, Yu, Nguyen, Sui, Plescia, Altieri, Conte: "Mitochondrial heat shock protein-90 modulates vascular smooth muscle cell survival and the vascular injury response in vivo." in: The American journal of pathology, Vol. 181, Issue 4, pp. 1151-7, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

de Souza, West, Rebert, de la Motte, Drazba, Fiocchi: "Increased levels of survivin, via association with heat shock protein 90, in mucosal T cells from patients with Crohn's disease." in: Gastroenterology, Vol. 143, Issue 4, pp. 1017-26.e9, 2012 (PubMed). (Sample species: Human).

Knauer, Heinrich, Bier, Habtemichael, Docter, Helling, Mann, Stauber: "An otoprotective role for the apoptosis inhibitor protein survivin." in: Cell death & disease, Vol. 1, pp. e51, 2011 (PubMed).

Lechler, Balakrishnan, Schaumburger, Grässel, Baier, Grifka, Straub, Renkawitz: "The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro." in: BMC musculoskeletal disorders, Vol. 12, pp. 150, 2011 (PubMed).

Habtemichael, Heinrich, Knauer, Schmidtmann, Bier, Docter, Brochhausen, Helling, Brieger, Stauber, Mann: "Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear." in: Molecular and cellular neurosciences, Vol. 45, Issue 3, pp. 297-305, 2010 (PubMed).

Habtemichael, Wünsch, Bier, Tillmann, Unruhe, Frauenknecht, Heinrich, Mann, Stauber, Knauer: "Cloning and functional characterization of the guinea pig apoptosis inhibitor protein Survivin." in: Gene, Vol. 469, Issue 1-2, pp. 9-17, 2010 (PubMed).

Brocardo, Lei, Tighe, Taylor, Mok, Henderson: "Mitochondrial targeting of adenomatous polyposis coli protein is stimulated by truncating cancer mutations: regulation of Bcl-2 and implications for cell survival." in: The Journal of biological chemistry, Vol. 283, Issue 9, pp. 5950-9, 2008 (PubMed).

Huang, Fletcher, Beeharry, Daniel, Kao, Yen, Muschel: "Abnormal cytokinesis after X-irradiation in tumor cells that override the G2 DNA damage checkpoint." in: Cancer research, Vol. 68, Issue 10, pp. 3724-32, 2008 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Levkau, Schäfers, Wohlschlaeger, von Wnuck Lipinski, Keul, Hermann, Kawaguchi, Kirchhof, Fabritz, Stypmann, Stegger, Flögel, Schrader, Fischer, Fischer, Hsieh, Ou, Mehrhof, Tiemann, Ghanem, Matus et al.: "Survivin determines cardiac function by controlling total cardiomyocyte number. ..." in: Circulation, Vol. 117, Issue 12, pp. 1583-93, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Hino, Uozaki, Inoue, Shintani, Ushiku, Sakatani, Takada, Fukayama: "Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A." in: Cancer research, Vol. 68, Issue 5, pp. 1427-35, 2008 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Ryan, ODonovan, Browne, OShea, Crown, Hill, McDermott, OHiggins, Duffy: "Expression of survivin and its splice variants survivin-2B and survivin-DeltaEx3 in breast cancer." in: British journal of cancer, Vol. 92, Issue 1, pp. 120-4, 2005 (PubMed).

Ansell, Arendt, Grote, Jelinek, Novak, Wellik, Remstein, Bennett, Fielding: "Inhibition of survivin expression suppresses the growth of aggressive non-Hodgkin's lymphoma." in: Leukemia, Vol. 18, Issue 3, pp. 616-23, 2004 (PubMed). (Sample species: Human). Further details: Western Blotting

Xu, Zhao, Ding, Tran, Zhang, Pandolfi, Chang: "Promyelocytic leukemia protein 4 induces apoptosis by inhibition of survivin expression." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 1838-44, 2004 (PubMed).

Ku, Kwak, Lee, Park, Lee, Lee: "Expression of survivin, a novel inhibitor of apoptosis, in superficial transitional cell carcinoma of the bladder." in: The Journal of urology, Vol. 171, Issue 2 Pt 1, pp. 631-5, 2004 (PubMed).

Koyama: "Differential expression of intracellular apoptotic signaling molecules in tumor and tumor-infiltrating lymphocytes during development of invasion and/or metastasis of gastric carcinoma." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2290-300, 2004 (PubMed).

Allen, Florell, Hanks, Alexander, Diedrich, Altieri, Grossman: "Survivin expression in mouse skin prevents papilloma regression and promotes chemical-induced tumor progression." in: Cancer research, Vol. 63, Issue 3, pp. 567-72, 2003 (PubMed).

Fortugno, Wall, Giodini, OConnor, Plescia, Padgett, Tognin, Marchisio, Altieri: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002 (PubMed).

Zhu, Fukuda, Cordis, Das, Maulik: "Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothelial cells by hypoxic preconditioning." in: FEBS letters, Vol. 508, Issue 3, pp. 369-74, 2001 (PubMed).