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Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) antibody

Details for Product No. ABIN153012
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Antigen
Synonyms CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, caspase7, cmh-1, ice-lap3, mch3, CASP7
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(169), (82), (63), (26), (24), (13), (12), (3), (3), (2), (1), (1), (1)
Host
Mouse
(138), (37), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(149), (72), (49), (45), (20), (20), (9), (8), (5), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
Shipping to United States (Change)
Catalog No. ABIN153012
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  • +1 404 474 4654
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Immunogen Full-length recombinant human Caspase 7
Clone Mch3 1-1-11
Isotype IgG1 kappa
Purification Ascites
Alternative Name Caspase-7
Background Caspases are cysteine proteases, expressed as inactive precursors, that mediateapoptosis by proteolysis of specific substrates. Caspases have the ability to cleave afteraspartic acid residues. There are two classes of caspases involved in apoptosis,initiators (activation by receptor cluster) and effectors (activation by mitochondrialpermeability transition). Proapoptotic signals autocatalytically activate initiatorcaspases, such as Caspase-8 and Caspase-9. Activated initiator caspases thenprocess effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cellcollapse. Alternate Names: anti-Apoptosis related cysteine peptidase antibody, anti-Apoptosis Related CysteineProtease antibody, anti-Apoptotic Protease MCH3 antibody, anti-CASP 7 antibody,anti-CMH1 antibody, anti-ICE LAP3 antibody, anti-ICE like Apoptotic Protease 3antibody, anti-MCH3 antibody.
Gene Symbol: CASP7
Gene ID 840, 12369, 64026
UniProt P55210
Research Area Apoptosis/Necrosis
Application Notes This Caspase 7 (Mch3 1-1-11) antibody is useful for Western blot, where a band can be seen at ~ 35 kDa.
Recommended dilutions: Western Blot 1:500-1:1000
Protocol Protocol specific for Caspase 7 Antibody Procedure Guide for NB 500-206 Monoclonal anti-Caspase 7 Western Protocol
1) Prepare samples for a NuPAGE gel and heat them at 70?C for 10 minutes.
2) Run a 4-12 % NuPAGE Bis-Tris gel, loading 50-100 mg of whole cell extract per lane, for 45 minutes at 200 Volts.
) Soak transfer sponges and gel 15 minutes and nitrocellulose membrane 5 minutes prior to transfer.
) Transfer the proteins to the nitrocellulose membrane for 1 hour at 30 Volts, in 4 degrees C transfer buffer.
) Block the membrane in TBST + 5 % non-fat dry milk, for 1 hour at room temperature (RT), shaking gently.
) Rinse the membrane twice with TBST.
) Incubate the membrane in anti-Caspase 7 [NB 500-206], diluted 1:500-1:1,000 in TBS + 1 % BSA, for 90 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in TBS + 1 % BSA for 35 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.*Positive control(s): HeLa whole cell extracts (cat# NB 800-PC1) Jurkat whole cell extracts (cat# NB 800-PC2)
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Kirsch, Doseff, Chau et al.: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999 (PubMed).

Blanc-Brude, Mesri, Wall et al.: "Therapeutic targeting of the survivin pathway in cancer: initiation of mitochondrial apoptosis and suppression of tumor-associated angiogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 9, Issue 7, pp. 2683-92, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN153012
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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