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Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP7 Antibody No. ABIN153012, Supplier: Log in to see
Antigen
  • xCaspase-7
  • caspase-7
  • caspase7
  • cmh-1
  • ice-lap3
  • mch3
  • CASP7
  • AI314680
  • CMH-1
  • ICE-IAP3
  • Mch3
  • mCASP-7
  • CASP-7
  • ICE-LAP3
  • LICE2
  • MCH3
  • Caspase-7
  • MCH3/SCA-2
  • SCA-2
  • caspase-7-like
  • caspase-7
  • caspase 7, apoptosis-related cysteine peptidase
  • caspase 7
  • LOC100068258
  • xCaspase-7
  • casp7
  • CASP7
  • Casp7
  • LOC100156777
  • LOC100101591
Alternatives
anti-Human Caspase 7 antibody for Immunoprecipitation
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 7 antibody is un-conjugated
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Application
Simple Western (SimWes), Western Blotting (WB)
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Options
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Immunogen Full-length recombinant human Caspase 7 [UniProt# P55210]
Clone Mch3 1-1-11
Isotype IgG1 kappa
Purification Unpurified
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background Gene Symbol: CASP7
Molecular Weight Theoretical MW: 35 kDa
Gene ID 840
UniProt P55210
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot 1:500-1:1000, Simple Western 1:500This Caspase 7 (Mch3 1-1-11) antibody is useful for Western blot, where a band can be seen at approx. 35 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Caspase 7 Antibody Procedure Guide for NB 500-206 Monoclonal anti-Caspase 7 Western Protocol
1) Prepare samples for a NuPAGE gel and heat them at 70?C for 10 minutes.
2) Run a 4-12 % NuPAGE Bis-Tris gel, loading 50-100 mg of whole cell extract per lane, for 45 minutes at 200 Volts.
) Soak transfer sponges and gel 15 minutes and nitrocellulose membrane 5 minutes prior to transfer.
) Transfer the proteins to the nitrocellulose membrane for 1 hour at 30 Volts, in 4 degrees C transfer buffer.
) Block the membrane in TBST + 5 % non-fat dry milk, for 1 hour at room temperature (RT), shaking gently.
) Rinse the membrane twice with TBST.
) Incubate the membrane in anti-Caspase 7 [NB 500-206], diluted 1:500-1:1,000 in TBS + 1 % BSA, for 90 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in TBS + 1 % BSA for 35 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.*Positive control(s): HeLa whole cell extracts (cat# NB 800-PC1) Jurkat whole cell extracts (cat# NB 800-PC2)
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (ABIN153012) Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [ABIN153012] - HeLa whole cell lysate.
Simple Western (SimWes) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (ABIN153012) Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [ABIN153012] - Simple Western lane v...
Product cited in: Badr, Hafez, Agha, Shouman: "The Combination of α-Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?" in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 4741694, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Blanc-Brude, Mesri, Wall, Plescia, Dohi, Altieri: "Therapeutic targeting of the survivin pathway in cancer: initiation of mitochondrial apoptosis and suppression of tumor-associated angiogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 9, Issue 7, pp. 2683-92, 2003 (PubMed).

Background publications Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999 (PubMed).

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998 (PubMed).