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Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) antibody

Antigen

Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A)
Synonyms CHAF1A, MGC152577, caip150, CAF-Ip150, wu:fd14g10, zgc:153930, LOC398222, xp150, chaf1a-A, CAF1, P150, CAF-1, CAF1B, CAF1P150, MGC71229
Clonality Monoclonal (SS11-13)
Host
Alternatives

Mouse
Reactivity
Alternatives

Fruit Fly (Drosophila melanogaster)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
4 references available
Catalog no. ABIN153013
Quantity 0.2 ml  (18.7 mg/ml)
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives back to top

Alternative name CAF-1 subunit A
Gene ID 10036
UniProt Q13111
Immunogen Recombinant human CAF-1p150.
Cross-Reactivity Fruit Fly (Drosophila melanogaster), Human, Yeast
Format Ascites
Isotype IgG1
Clone SS11-13
Description Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It isrequired for inheritance of epigenetically determined chromosomal states, and in vitro, itpromotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 isa complex of three subunits, p150, p60, and p48. This complex is localized to thenucleus of human cells.Both the composition and the biochemical activity of CAF-1 are conserved amonghuman, yeast, and Drosophila. Alternate Names: anti-p150 CAF1 antibody.
Specificity Specific for CAF-1p150. Species Reactivity: Human.

Application Details
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Application Notes Suggested working dilutions: Western Blot 1:500-1:1,000
Concentration 18.7 mg/ml
Purification Ascites
Buffer Preservative: 0.1% sodium azide.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).

Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).

Green, Almouzni: "Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo." in: The EMBO journal, Vol. 22, Issue 19, pp. 5163-74, 2003 (PubMed).

Naryzhny: "Proliferating cell nuclear antigen: a proteomics view." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 23, pp. 3789-808, 2008 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A)", type "Antibodies"
Hosts Rabbit (25), Mouse (5)
Reactivities Human (30), Mouse (Murine) (23), Rat (Rattus) (22), Cow (Bovine) (19), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Cat (Feline) (1), Chicken (1)
Applications Immunofluorescence (IF) (18), Western Blotting (WB) (13), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (IHC) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes C-Term (1)