Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) antibody

Details for Product No. ABIN153013
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Antigen
Synonyms CHAF1A, CAF-1, CAF1, CAF1B, CAF1P150, P150, AL023013, AL024058, Cac1p, CAF-1P150, CAF-I-p150, CAF-Ip150, caip150, wu:fd14g10, zgc:153930, chaf1a-b, xp150
Reactivity
Human
(47), (24), (23), (12), (12), (12)
Host
Mouse
(29), (19)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(35), (18), (10), (8), (8), (6), (3), (2), (1)
Pubmed 4 references available
Quantity 0.2 mL
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Catalog No. ABIN153013
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Immunogen Recombinant human CHAF1A
Clone SS 1 1-13
Isotype IgG1
Purification Ascites
Alternative Name CAF-1 subunit A
Background Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It isrequired for inheritance of epigenetically determined chromosomal states, and in vitro, itpromotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 isa complex of three subunits, p150, p60, and p48. This complex is localized to thenucleus of human cells.Both the composition and the biochemical activity of CAF-1 are conserved amonghuman, yeast, and Drosophila. Alternate Names: anti-p150 CAF1 antibody.
Gene Symbol: CHAF1A
Gene ID 10036
UniProt Q13111
Application Notes This CHAF1A (SS 1 1-13) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band at 150 kDa is seen.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:500-1:1000
Protocol Protocol specific for Caf1p150 Antibody Procedure Guide for NB 500-206 Monoclonal anti-Caspase 7 Western Protocol
1) Prepare samples for a NuPAGE gel and heat them at 70 degrees C for 10 minutes.
2) Run a 4-12 % NuPAGE Bis-Tris gel, loading 50-100 mg of whole cell extract per lane, for 45 minutes at 200 Volts.
) Soak transfer sponges and gel 15 minutes and nitrocellulose membrane 5 minutes prior to transfer.
) Transfer the proteins to the nitrocellulose membrane for 1 hour at 30 Volts, in 4 degrees C transfer buffer.
) Block the membrane in TBST + 5 % non-fat dry milk, for 1 hour at room temperature (RT), shaking gently.
) Rinse the membrane twice with TBST.
) Incubate the membrane in anti-Caspase 7 [NB 500-206], diluted 1:500-1:1,000 in TBS + 1 % BSA, for 90 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in TBS + 1 % BSA for 35 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.*Positive control(s): HeLa whole cell extracts (cat# NB 800-PC1) Jurkat whole cell extracts (cat# NB 800-PC2)
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).

Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).

Green, Almouzni: "Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo." in: The EMBO journal, Vol. 22, Issue 19, pp. 5163-74, 2003 (PubMed).

Naryzhny: "Proliferating cell nuclear antigen: a proteomics view." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 23, pp. 3789-808, 2008 (PubMed).

Validation Images
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