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CHAF1A antibody (Chromatin Assembly Factor 1, Subunit A (p150))

Details for Product anti-CHAF1A Antibody No. ABIN153013, Supplier: Log in to see
Antigen
  • AL023013
  • AL024058
  • Cac1p
  • CAF-1
  • CAF-1P150
  • CAF-I-p150
  • CAF-Ip150
  • CAF1
  • CAF1B
  • CAF1P150
  • caip150
  • CHAF1A
  • chaf1a-b
  • P150
  • wu:fd14g10
  • xp150
  • zgc:153930
Reactivity
Human
54
22
20
Host
Mouse
33
19
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CHAF1A antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
38
20
11
8
7
6
3
3
1
1
Supplier
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Supplier Product No.
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Immunogen Recombinant human CHAF1A [UniProt# Q13111]
Clone SS 1 1-13
Isotype IgG1
Purification Ascites
Alternative Name CHAF1A (CHAF1A Antibody Abstract)
Background Gene Symbol: CHAF1A
Molecular Weight Theoretical MW: 150 kDa
Gene ID 10036
UniProt Q13111
Application Notes Western Blot 1:500-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Caf1p150 Antibody Procedure Guide for NB 500-206 Monoclonal anti-Caspase 7 Western Protocol
1) Prepare samples for a NuPAGE gel and heat them at 70 degrees C for 10 minutes.
2) Run a 4-12 % NuPAGE Bis-Tris gel, loading 50-100 mg of whole cell extract per lane, for 45 minutes at 200 Volts.
) Soak transfer sponges and gel 15 minutes and nitrocellulose membrane 5 minutes prior to transfer.
) Transfer the proteins to the nitrocellulose membrane for 1 hour at 30 Volts, in 4 degrees C transfer buffer.
) Block the membrane in TBST + 5 % non-fat dry milk, for 1 hour at room temperature (RT), shaking gently.
) Rinse the membrane twice with TBST.
) Incubate the membrane in anti-Caspase 7 [NB 500-206], diluted 1:500-1:1,000 in TBS + 1 % BSA, for 90 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in TBS + 1 % BSA for 35 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.*Positive control(s): HeLa whole cell extracts (cat# NB 800-PC1) Jurkat whole cell extracts (cat# NB 800-PC2)
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-CHAF1A antibody (Chromatin Assembly Factor 1, Subunit A (p150)) (ABIN153013) anti-Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) antibody
Immunofluorescence (IF) image for anti-CHAF1A antibody (Chromatin Assembly Factor 1, Subunit A (p150)) (ABIN153013) Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [ABIN153013] - Im...
Product cited in: Rivera, Saavedra, Alvarez, Díaz-Celis, Ugalde, Li, Forné, Gurard-Levin, Almouzni, Imhof, Loyola: "Methylation of histone H3 lysine 9 occurs during translation." in: Nucleic acids research, Vol. 43, Issue 19, pp. 9097-106, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Naryzhny: "Proliferating cell nuclear antigen: a proteomics view." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 23, pp. 3789-808, 2008 (PubMed).

Mocquet, Lainé, Riedl, Yajin, Lee, Egly: "Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step." in: The EMBO journal, Vol. 27, Issue 1, pp. 155-67, 2008 (PubMed). (Sample species: Human).

Ghosh, Dohi, Kang, Altieri: "Hsp60 regulation of tumor cell apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 5188-94, 2008 (PubMed).

Galvani, Courbeyrette, Agez, Ochsenbein, Mann, Thuret: "In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3672-85, 2008 (PubMed).

Naryzhny, Zhao, Lee: "Proliferating cell nuclear antigen (PCNA) may function as a double homotrimer complex in the mammalian cell." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13888-94, 2005 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Jiao, Bachrati, Pedrazzi, Kuster, Petkovic, Li, Egli, Hickson, Stagljar: "Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1." in: Molecular and cellular biology, Vol. 24, Issue 11, pp. 4710-9, 2004 (PubMed).

Green, Almouzni: "Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo." in: The EMBO journal, Vol. 22, Issue 19, pp. 5163-74, 2003 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunoprecipitation,Immunofluorescence

Background publications Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).