CAF-1 Subunit antibody

Details for Product No. ABIN153017
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Fruit Fly (Drosophila melanogaster), Human, Yeast
(11), (2), (1)
(10), (3)
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(11), (10), (5), (3), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN153017
Quantity 50 µL
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Immunogen Recombinant human CAF-1p60.
Clone SS241-68
Isotype IgG1
Specificity Species Reactivity: Human. Localization: Nuclear.
Cross-Reactivity Fruit Fly (Drosophila melanogaster), Human, Yeast
Purification Ascites
Background Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It isrequired for inheritance of epigenetically determined chromosomal states, and in vitro, itpromotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 isa complex of three subunits, p150, p60, and p48. This complex is localized to thenucleus of human cells.Both the composition and the biochemical activity of CAF-1 are conserved amonghuman, yeast, and Drosophila. Alternate Names: anti-p60 CAF1 antibody, CHAF1B antibody Chromatin assembly factor 1 subunit B antibody, anti-Chromatin assembly factor Iantibody, anti-M phase phosphoprotein 7 antibody, anti-MPHOSPH7 antibody.
Gene ID 8208
UniProt Q13112
Application Notes Suggested working dilutions: immunoprecipitation 0.1-1 µg
Restrictions For Research Use only
Format Liquid
Concentration 31.5 mg/ml
Buffer Ascitic fluid. Preservative: 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage -20 °C
General Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).

Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).

Hosts (10), (3)
Reactivities (11), (2), (1)
Applications (11), (10), (5), (3), (1), (1), (1)
Epitopes (1), (1)
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