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CHAF1B antibody (Chromatin Assembly Factor 1, Subunit B (p60))

Details for Product anti-CHAF1B Antibody No. ABIN153017, Supplier: Log in to see
Antigen
  • 2600017H24Rik
  • C76145
  • CAF-1
  • caf-1
  • CAF-1P60
  • CAF-IP60
  • CAF-I p60
  • CAF-Ip60
  • CAF1
  • CAF1A
  • CAF1P60
  • CHAF1B
  • MPHOSPH7
  • MPP7
  • wu:fd07c09
  • wu:fe36g06
  • wu:fv38g09
  • zgc:56096
Reactivity
Human, Mouse (Murine), Primate
41
12
6
3
1
1
1
1
1
Host
Mouse
28
15
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
32
18
13
12
8
7
6
1
Supplier
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Immunogen Recombinant human CHAF1B [UniProt# Q13112]
Clone SS 24 1-68
Isotype IgG1
Purification Ascites
Alternative Name CHAF1B (CHAF1B Antibody Abstract)
Background Gene Symbol: CHAF1B
Molecular Weight Theoretical MW: 60 kDa
UniProt Q13112
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 0.1-1 μg
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunoprecipitation protocol specific for CAF-1 p60 Antibody Procedure Guide for Monoclonal anti- CAF-1 p60 (clone 24)Catalog# NB 500-211Immunoprecipitation Procedure1) Cells were prepared in 0.4-1 % NP-40 lysis buffer.
2) After osmotic shock and nuclear extractions of the cells, use the following buffer: 25mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, and 0.01 % NP-
40. 3) Clarify the extracts with two successive 5 minute clearing spins (in a microfuge).
) Preclear the extracts with some protein G beads (~20 µL). 5) Incubate the extracts with ~ 0.1-1ug of anti-CAF-1p60-24 [NB 500-211]/ control antibody for 1-3 hours. 6) Spin the antibody/extract for 5 minutes.
) Incubate the antibody/extract with protein G beads, rotating for 1-2 hours.
) Wash the beads, twice, for 5 minutes, at low ionic strength [150mM MaCl] in 1ml volume (*Optional: wash again, twice, at high ionic strength [ 400mM NaCl]).
) Resuspend the washed beads in Laemlli sample buffer and boil for 5 minutes. NOTE: a. All steps for IP are performed at 4C.b. CAF-1p60 is phosphorylated and contains a PEST sequence. Therefore, it is recommended that protease and phophatase inhibitors be added to all buffers.c. The protocol for crosslinking CAF-1p60 to protein G beads was obtained from the Harlow and Lane book, Using Antibodies.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-CHAF1B antibody (Chromatin Assembly Factor 1, Subunit B (p60)) (ABIN153017) anti-Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B) antibody
Immunofluorescence (IF) image for anti-CHAF1B antibody (Chromatin Assembly Factor 1, Subunit B (p60)) (ABIN153017) Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [ABIN153017] - I...
Product cited in: Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence