CHAF1B antibody (Chromatin Assembly Factor 1, Subunit B (p60))

Details for Product anti-CHAF1B Antibody No. ABIN153017, Supplier: Log in to see
Antigen
  • CHAF1B
  • wu:fd07c09
  • wu:fe36g06
  • wu:fv38g09
  • zgc:56096
  • CAF-1
  • CAF-IP60
  • CAF1
  • CAF1A
  • CAF1P60
  • MPHOSPH7
  • MPP7
  • 2600017H24Rik
  • C76145
  • CAF-I p60
  • CAF-Ip60
  • caf-1
  • CAF-1P60
  • chromatin assembly factor 1, subunit B (p60)
  • chromatin assembly factor 1, subunit B
  • CHAF1B
  • chaf1b
  • LOC100223590
  • Chaf1b
Reactivity
Human, Mouse (Murine), Primate
47
17
10
5
4
2
2
2
2
1
1
1
1
1
Host
Mouse
30
19
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
42
19
15
12
11
10
7
2
Options
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Immunogen Recombinant human CHAF1B [UniProt# Q13112]
Clone SS 24 1-68
Isotype IgG1
Purification Unpurified
Alternative Name CHAF1B (CHAF1B Antibody Abstract)
Background Gene Symbol: CHAF1B
Molecular Weight Theoretical MW: 60 kDa
Gene ID 8208
UniProt Q13112
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 0.1-1 μgThis CHAF1B (SS 24 1-68) antibody is useful for Immunoprecipitation, Western blot and Immunocytochemistry/Immunofluorescence. In WB a band can be seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunoprecipitation protocol specific for CAF-1 p60 Antibody Procedure Guide for Monoclonal anti- CAF-1 p60 (clone 24)Catalog# NB 500-211Immunoprecipitation Procedure1) Cells were prepared in 0.4-1 % NP-40 lysis buffer.
2) After osmotic shock and nuclear extractions of the cells, use the following buffer: 25mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, and 0.01 % NP-
40. 3) Clarify the extracts with two successive 5 minute clearing spins (in a microfuge).
) Preclear the extracts with some protein G beads (~20 µL). 5) Incubate the extracts with ~ 0.1-1ug of anti-CAF-1p60-24 [NB 500-211]/ control antibody for 1-3 hours. 6) Spin the antibody/extract for 5 minutes.
) Incubate the antibody/extract with protein G beads, rotating for 1-2 hours.
) Wash the beads, twice, for 5 minutes, at low ionic strength [150mM MaCl] in 1ml volume (*Optional: wash again, twice, at high ionic strength [ 400mM NaCl]).
) Resuspend the washed beads in Laemlli sample buffer and boil for 5 minutes. NOTE: a. All steps for IP are performed at 4C.b. CAF-1p60 is phosphorylated and contains a PEST sequence. Therefore, it is recommended that protease and phophatase inhibitors be added to all buffers.c. The protocol for crosslinking CAF-1p60 to protein G beads was obtained from the Harlow and Lane book, Using Antibodies.
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-CHAF1B antibody (Chromatin Assembly Factor 1, Subunit B (p60)) (ABIN153017) anti-Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B) antibody
Immunofluorescence (IF) image for anti-CHAF1B antibody (Chromatin Assembly Factor 1, Subunit B (p60)) (ABIN153017) Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [ABIN153017] - I...
Product cited in: Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence