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|+1 888 205 9894 (TF)|
CAF-1 Subunit antibody
Fruit Fly (Drosophila melanogaster)
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
|Quantity||0.1 ml (16.3 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Recombinant human CAF-1p60.|
|Cross-Reactivity||Fruit Fly (Drosophila melanogaster), Human, Yeast|
|Description||Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It isrequired for inheritance of epigenetically determined chromosomal states, and in vitro, itpromotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 isa complex of three subunits, p150, p60, and p48. This complex is localized to thenucleus of human cells.Both the composition and the biochemical activity of CAF-1 are conserved amonghuman, yeast, and Drosophila. Alternate Names: anti-p60 CAF1 antibody.|
|Specificity||Specific for CAF-1p60. Species Reactivity: Recognizes human CAF-1p60. Other species have not been tested. Localization: Nuclear.|
|Application Notes||Western analysis: A band is seen at 60 kDa representing CAF-1p60. In addition, thisantibody may be used for immunoprecipitation (100-500 ng of IgG).|
|Buffer||Ascitic fluid. Preservative: 0.1% sodium azide.|
|Storage||Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).
Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).
Green, Almouzni: "Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo." in: The EMBO journal, Vol. 22, Issue 19, pp. 5163-74, 2003 (PubMed).
Galvani, Courbeyrette, Agez et al.: "In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3672-85, 2008 (PubMed).