Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (N-Term) antibody

Antigen: Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10)

Synonyms: TL2, APO2L, CD253, TRAIL, Apo-2L, Ly81, Trail, APO-2L, AI448571, A330042I21Rik, TRAIL-LIKE, TNFSF10, tl2, apo2l, cd253, trail, apo-2l, trail1, xtrail1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN153025

Additional Information

Alternative name: CD253 / TRAIL

Gene ID: 8743

Cross-Reactivity: Human

Description: Apoptosis or programmed cell death is induced in cells by a group of death domaincontaining receptors. Binding of ligand to these receptors sends signals that activatemembers of the caspase family of proteases. The signals ultimately cause degradationof chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis inducedligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors,DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and acytoplasmic death domain. In addition, two decoy receptors for TRAIL havealso been identified. These receptors, designated DcR1 and DcR2, lack the deathdomain. Binding of TRAIL to either of these receptors, therefore, does not transmit thedeath signal. Thus, these receptors represent a novel way of regulating cell sensitivity toa pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly inspleen, lung, and prostate but also in many other tissues.TRAIL is a type II membrane protein from the TNF family. The human form of theprotein is 281 amino acids in length, whereas the murine form is 291 amino acids. Thetwo forms share 65% identity. Recombinant human TRAIL monomer has a molecularmass of approximately 21 kDa. Due to glycosylation, recombinant human TRAILmigrates as a 24 kDa protein in SDS PAGE. Alternate Names: anti-Apo 2 ligand antibody, anti-Apo 2L antibody, anti-APO2L antibody, anti-CD253antibody, anti-TL2 antibody, anti-TNF Related Apoptosis Inducing Ligand antibody,anti-TNFSF10 antibody, anti-TRAIL antibody, anti-TRAIL PEN antibody, anti-TumorNecrosis Factor (ligand) Superfamily Member 10 antibody.

Specificity: Reacts with residues [SNTLSSPNSKNE] of the N terminus of the TRAIL protein. Species Reactivity: Human.

Application Details

Application Notes: Western blot- Use at 1:500 to 1:1,000 dilution. However, the investigator shoulddetermine the optimal dilution for a specific application such as immunohistochemistry

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: 0.1 mg in 0.1 ml (1 mg/ml) in PBS containing 0.02% sodium azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Publications

Wiley, Schooley, Smolak et al.: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, 1996 (PubMed).

Herzer, Hofmann, Teufel et al.: "IFN-alpha-induced apoptosis in hepatocellular carcinoma involves promyelocytic leukemia protein and TRAIL independently of p53." in: Cancer research, Vol. 69, Issue 3, pp. 855-62, 2009 (PubMed).