Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) antibody

Details for Product No. ABIN153025
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Antigen
Synonyms TRAIL, tl2, apo2l, cd253, trail, apo-2l, trail1, xtrail1, APO2L, Apo-2L, CD253, TL2, A330042I21Rik, AI448571, APO-2L, Ly81, Trail
Reactivity
Human
(187), (51), (23), (2), (2), (1)
Host
Rabbit
(126), (63), (25), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(25), (14), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(132), (81), (78), (31), (20), (19), (13), (12), (10), (7), (7), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN153025
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Immunogen residues
Specificity Reacts with residues [SNTLSSPNSKNE] of the N terminus of the TRAIL protein.
Cross-Reactivity (Details) Mouse reactivity reported in scientific literature (PMID: 1914164).
Purification affinity purified
Alternative Name CD253 / TRAIL
Background Apoptosis or programmed cell death is induced in cells by a group of death domaincontaining receptors. Binding of ligand to these receptors sends signals that activatemembers of the caspase family of proteases. The signals ultimately cause degradationof chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis inducedligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors,DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and acytoplasmic death domain. In addition, two decoy receptors for TRAIL havealso been identified. These receptors, designated DcR1 and DcR2, lack the deathdomain. Binding of TRAIL to either of these receptors, therefore, does not transmit thedeath signal. Thus, these receptors represent a novel way of regulating cell sensitivity toa pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly inspleen, lung, and prostate but also in many other tissues.TRAIL is a type II membrane protein from the TNF family. The human form of theprotein is 281 amino acids in length, whereas the murine form is 291 amino acids. Thetwo forms share 65% identity. Recombinant human TRAIL monomer has a molecularmass of approximately 21 kDa. Due to glycosylation, recombinant human TRAILmigrates as a 24 kDa protein in SDS PAGE. Alternate Names: anti-Apo 2 ligand antibody, anti-Apo 2L antibody, anti-APO2L antibody, anti-CD253antibody, anti-TL2 antibody, anti-TNF Related Apoptosis Inducing Ligand antibody,anti-TNFSF10 antibody, anti-TRAIL antibody, anti-TRAIL PEN antibody, anti-TumorNecrosis Factor (ligand) Superfamily Member 10 antibody.
Gene Symbol: TNFSF10
Gene ID 8743
Application Notes Western blot- Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry
Recommended dilutions: Western Blot 1:100-1:2000
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Wiley, Schooley, Smolak et al.: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, 1996 (PubMed).

Herzer, Hofmann, Teufel et al.: "IFN-alpha-induced apoptosis in hepatocellular carcinoma involves promyelocytic leukemia protein and TRAIL independently of p53." in: Cancer research, Vol. 69, Issue 3, pp. 855-62, 2009 (PubMed).

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