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BrdU antibody (Bromodeoxyuridine)

Details for Product anti-BrDU Antibody No. ABIN153035, Supplier: Log in to see
Antigen
Reactivity
Chemical
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5
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Host
Sheep
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Clonality
Polyclonal
Conjugate
This BrdU antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
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Options
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Immunogen Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH).
Specificity BrdU
Purification Protein G purified
Alternative Name Bromodeoxyuridine/BrdU
Target Type Chemical
Application Notes Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 25-100 μg/mL, Immunohistochemistry-Paraffin 10 μg/mL, Immunohistochemistry-Frozen 10 μg/mLThis BrdU antibody is useful for ICC/IF, IHC-frozen, IHC-paraffin, and IP.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for BrdU Antibody Immunohistochemistry protocol for Bromodeoxyuridine (BrdU) Polyclonal AntibodyOriginal antigenic studies performed by Dr David Stollar, Tufts University, Boston, MAABSTRACTNew methods for double and triple colour labeling using monoclonal antibodies to the proliferation-associated markers 5-methly-cytosine, BrdU and Ki67 are described. In order to make incorporated 5-methyl-cytosine or BrdU accessible to most antibodies, mild denaturation of the DNA is needed, and this is usually obtained by exposing the cells to acid or base. This procedure destroys most cellular antigen, including nuclear TdT and Ki
67. In this study, we show that fixation in cold methanol instead of 70 % ethanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail. This method is optimal for detection of nuclear Ki67 and TdT. These reagents, together with antibodies to membrane assigns can be included in triple colour labeling using second layers conjugated to FITC, TRITC and colloidal gold. With these methods it is now possible to characterize the phenotype of dividing cell populations such as precursors in central lymphoid tissues and germinal centre blasts in peripheral lymphoid organs.Reference for tissue staining with anti-BrdUCampana D, Coustan-Smith E, Janossy G, Department of Immunology, Royal Free Hospital School of Medicine, London, UK. J Immunol Methods 107(1):79-88 1988 Feb 24 Double and triple staining methods for studying the proliferative activity of human B and T lymphoid cells AbstractFor best results on unconjugated antibody, use the product at a concentration of 25 to 100 µg/mL. Nearly complete immunoprecipitation was obtained at concentrations of 100 to 500ug/mL. The product has also been tested for utility for staining of bromodeoxyuridine incorporated into DNA of replicating cells. When utilized at a purified concentration of 10 µg/mL, the product is comparable to commercially available monoclonal antibodies commonly used for the same purpose.A key issue is that it is necessary to denature the DNA so as to expose the base to the antibodies. One protocol is: Sachiko Matsuuraa and Kazuo Suzukia Immunohistochemical Analysis of DNA Synthesis During Chronic Stimulation with Isoproterenol in Mouse Submandibular Gland . Journal of Histochemistry and Cytochemistry, Vol. 45, 1137-1146, Immunohistochemistry DNA-synthesizing cells were detected by peroxidase-anti-peroxidase (PAP) immunostaining with anti-BrdU antibodies by the method of Harms et al. 1986 , which was slightly modified as follows: Sections were treated with 2 N HCl at 60C for 15 min and washed in running water for 15 min. After dehydration in an ethanol series, the sections were etched with xylene (15 min, twice). Nuclear proteins, which mask incorporated BrdU, were digested with 0.025 % protease (Type V, Sigma) in PBS at 37C for 10 min, and the sections were then washed with cold PBS three times for 5 min.
Restrictions For Research Use only
Format Liquid
Concentration 3.2 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
 image for anti-BrdU antibody (Bromodeoxyuridine) (ABIN153035) anti-Bromodeoxyuridine (BrDU) antibody
Immunofluorescence (IF) image for anti-BrdU antibody (Bromodeoxyuridine) (ABIN153035) Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [ABIN153035] ...
Product cited in: Lin, Zu, Yang, Tsai, Shyu, Chen, Weng, Chen, Wang: "IL-1β-Induced Mesenchymal Stem Cell Migration Involves MLCK Activation via PKC Signaling." in: Cell transplantation, Vol. 24, Issue 10, pp. 2011-28, 2015 (PubMed). Further details: Immunohistochemistry (Frozen Sections)

Puzzo, Bizzoca, Privitera, Furnari, Giunta, Girolamo, Pinto, Gennarini, Palmeri: "F3/Contactin promotes hippocampal neurogenesis, synaptic plasticity, and memory in adult mice." in: Hippocampus, Vol. 23, Issue 12, pp. 1367-82, 2013 (PubMed). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Criswell, Cook, Morse, Lawton, Somps, Obert, Roy, Sokolowski, Koza-Taylor, Colangelo, Navetta, Brady, Pegg, Wojcinski, Rahbari, Duddy, Anderson: "Pregabalin induces hepatic hypoxia and increases endothelial cell proliferation in mice, a process inhibited by dietary vitamin E supplementation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 128, Issue 1, pp. 42-56, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Tveden-Nyborg, Vogt, Schjoldager, Jeannet, Hasselholt, Paidi, Christen, Lykkesfeldt: "Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs." in: PLoS ONE, Vol. 7, Issue 10, pp. e48488, 2012 (PubMed). (Sample species: Guinea Pig). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Dunlap, Silva, Chung: "Environmental complexity, seasonality and brain cell proliferation in a weakly electric fish, Brachyhypopomus gauderio." in: The Journal of experimental biology, Vol. 214, Issue Pt 5, pp. 794-805, 2011 (PubMed). Further details: Immunohistochemistry

Zhang, Zhang, Dutta, Argaw, Bonnamain, Seto, Braun, Zameer, Hayot, Lòpez, Raine, John: "Proapoptotic and antiapoptotic actions of Stat1 versus Stat3 underlie neuroprotective and immunoregulatory functions of IL-11." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 3, pp. 1129-41, 2011 (PubMed).

Tulloch, Afanador, Mexhitaj, Ghazaryan, Garzagongora, Angulo: "A single high dose of methamphetamine induces apoptotic and necrotic striatal cell loss lasting up to 3 months in mice." in: Neuroscience, Vol. 193, pp. 162-9, 2011 (PubMed).

You, Tariq, Kublin, Zoukhri: "Detection of BrdU-label retaining cells in the lacrimal gland: implications for tissue repair." in: Cell and tissue research, Vol. 346, Issue 3, pp. 317-26, 2011 (PubMed).

Gurfein, Zhang, López, Argaw, Zameer, Moran, John: "IL-11 regulates autoimmune demyelination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 7, pp. 4229-40, 2009 (PubMed).

Zhang, Argaw, Gurfein, Zameer, Snyder, Ge, Lu, Rowitch, Raine, Brosnan, John: "Notch1 signaling plays a role in regulating precursor differentiation during CNS remyelination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19162-7, 2009 (PubMed).

Wang, Tang, Maul, Yuan: "Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: dual role of replication and transcription activator." in: Journal of virology, Vol. 80, Issue 24, pp. 12171-86, 2006 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Tokuda, Kadokawa, Kurahashi, Marunouchi: "CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes." in: Biology of reproduction, Vol. 76, Issue 1, pp. 130-41, 2006 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Cooper, Isacson et al.: "Intrastriatal transforming growth factor alpha delivery to a model of Parkinson's disease induces proliferation and migration of endogenous adult neural progenitor cells without differentiation into ..." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 41, pp. 8924-31, 2004 (PubMed).

Reference: Morris, Solomon: "BRCA1 : BARD1 induces the formation of conjugated ubiquitin structures, dependent on K6 of ubiquitin, in cells during DNA replication and repair." in: Human molecular genetics, Vol. 13, Issue 8, pp. 807-17, 2004 (PubMed).

Campana, Coustan-Smith, Janossy: "Double and triple staining methods for studying the proliferative activity of human B and T lymphoid cells." in: Journal of immunological methods, Vol. 107, Issue 1, pp. 79-88, 1988 (PubMed).