Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Details for Product No. ABIN153037
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Antigen
Synonyms API4, EPR-1, AAC-11, Api4, TIAP, survivin40, AP14
Reactivity
Human
(67), (25), (8), (1), (1), (1), (1)
Host
Mouse
(68), (15), (2)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(69), (26), (25), (18), (17), (13), (7), (5), (4), (3), (1), (1), (1)
Pubmed 7 references available
Quantity 0.1 mL
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Catalog No. ABIN153037
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Immunogen Full length recombinant human Survivin
Clone 32-1
Isotype IgG1 kappa
Specificity NB500-237 is specific for the nuclear form of survivin.
Purification Protein G purified
Alternative Name IAP4 / BIRC5
Background Regulated inhibition of programmed cell death (apoptosis) preserves normalhomeostasis and tissue and organ morphogenesis. Aberrations in this processcontribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have beenfound in various species, including human. IAP proteins contain one/three Cys/Hisbaculovirus IAP repeats plus a c-terminus RING finger and are thought to block anevolutionary conserved step in apoptosis.Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expressionis turned off during fetal development and is not found in non-neoplastic adult humantissues. Survivin becomes abundantly re-expressed in transformed cells and in all ofthe most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division,governing a checkpoint involved in cytokinesis while also suppressing apoptosis.Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) ofadult rats following traumatic brain injury. Survivin has been found co-expressed withNeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affectregulation of neural cell proliferative responses after brain injury. Alternate Names: anti-API4 antibody, anti-Apoptosis Inhibitor 4 antibody, anti-Apoptosis inhibitor survivinantibody, anti-Baculoviral IAP repeat containing 5 antibody, anti-BIRC 5 antibody,anti-EPR 1 antibody, anti-IAP4 antibody, anti-SVV antibody, anti-TIAP antibody,anti-BIRC5 antibody.
Gene Symbol: BIRC5
Gene ID 332
UniProt O15392
Research Area Cancer, Apoptosis/Necrosis, Neurology
Application Notes This Survivin (32.1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. ELISA was reported in scientific literature. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. *The investigator should determine the optimal working dilution for a specific application.
Recommended dilutions: ELISA, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 20 µg/mL, Immunohistochemistry-Paraffin 20 µg/mL, Immunoprecipitation, Western Blot 1:1000
Protocol Protocol specific for Survivin Antibody IHC-FFPE sections I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.9 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody Staining of human lung, cancer using ABIN153037.
General Ambrosini, Adida, Sirugo et al.: "Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 11177-82, 1998 (PubMed).

Li, Ambrosini, Chu et al.: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999 (PubMed).

Li, Ackermann, Bennett et al.: "Pleiotropic cell-division defects and apoptosis induced by interference with survivin function." in: Nature cell biology, Vol. 1, Issue 8, pp. 461-6, 2000 (PubMed).

Grossman, McNiff, Li et al.: "Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma." in: The Journal of investigative dermatology, Vol. 113, Issue 6, pp. 1076-81, 2000 (PubMed).

Adida, Haioun, Gaulard et al.: "Prognostic significance of survivin expression in diffuse large B-cell lymphomas." in: Blood, Vol. 96, Issue 5, pp. 1921-5, 2000 (PubMed).

Fortugno, Wall, Giodini et al.: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002 (PubMed).

Giodini, Kallio, Wall et al.: "Regulation of microtubule stability and mitotic progression by survivin." in: Cancer research, Vol. 62, Issue 9, pp. 2462-7, 2002 (PubMed).

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