Survivin antibody (BIRC5) (Nuclear)

Details for Product anti-BIRC5 Antibody No. ABIN153037, Supplier: Log in to see
Antigen
  • bir5a
  • survivin
  • LOC398389
  • BIRC5
  • BIR1
  • Su2
  • Survivin
  • Survivin1
  • XBIR1
  • api4
  • epr-1
  • svv1
  • xSurvivin1
  • xsurv
  • SIX
  • Su1
  • svv2
  • birc5
  • xSurvivin
  • xSurvivin2
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • baculoviral IAP repeat-containing 5a
  • survivin
  • baculoviral IAP repeat-containing 5
  • baculoviral IAP repeat-containing 5, gene 1
  • baculoviral IAP repeat containing 5
  • birc5a
  • LOC398389
  • BIRC5
  • birc5.1
  • birc5.2
  • AaeL_AAEL012446
  • CpipJ_CPIJ001663
  • CpipJ_CPIJ002689
  • CpipJ_CPIJ006694
  • Birc5
Alternatives
anti-Human Survivin antibody for Biochemical Assay
Epitope
Nuclear
65
25
19
19
17
16
15
15
13
7
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human
403
167
162
34
20
19
12
4
2
2
1
Host
Mouse
290
120
16
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Survivin antibody is un-conjugated
23
15
14
12
12
12
11
10
8
8
7
6
4
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
332
187
163
142
132
105
88
33
32
26
7
5
3
3
3
3
2
2
2
2
1
1
1
Options
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Clone 32-1
Isotype IgG1 kappa
Specificity This antibody is specific for the nuclear form of survivin.
Purification Protein G purified
Alternative Name Survivin (BIRC5 Antibody Abstract)
Background Gene Symbol: BIRC5
Molecular Weight Theoretical MW: 16.5 kDa
Gene ID 332
UniProt O15392
Research Area Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Pathways Nuclear Hormone Receptor Binding
Application Notes Western Blot 1:1000, ELISA, Immunohistochemistry 20 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 20 μg/mLThis Survivin (32.1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. ELISA was reported in scientific literature. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. *The investigator should determine the optimal working dilution for a specific application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Survivin Antibody IHC-FFPE sections I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.9 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Survivin antibody (BIRC5) (Nuclear) (ABIN153037) Staining of human lung, cancer using ABIN153037.
Product cited in: Guvenc, Pavlyukov, Joshi, Kurt, Banasavadi-Siddegowda, Mao, Hong, Yamada, Kwon, Bhasin, Chettiar, Kitange, Park, Sarkaria, Li, Shakhparonov, Nakano: "Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 19, Issue 3, pp. 631-42, 2013 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Hori, Miki, Okamoto, Yazama, Konishi, Kaneko, Shimamoto, Ota, Temme, Tatsuka: "The detergent-soluble cytoplasmic pool of survivin suppresses anoikis and its expression is associated with metastatic disease of human colon cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55710, 2013 (PubMed). Further details: Western Blotting

Lechler, Renkawitz, Campean, Balakrishnan, Tingart, Grifka, Schaumburger: "The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro." in: BMC cancer, Vol. 11, pp. 120, 2011 (PubMed).

Lechler, Schaumburger, Köck, Balakrishnan, Doukas, Prantl, Grifka: "The oncofetal gene survivin promotes cell proliferation and survival in primary human osteoblastic cells." in: Calcified tissue international, Vol. 89, Issue 3, pp. 211-20, 2011 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Qi, Tuncel, Aoki, Tanaka, Oka, Kaneko, Okamoto, Tatsuka, Nakai, Shimamoto: "Intracellular localization of survivin determines biological behavior in colorectal cancer." in: Oncology reports, Vol. 22, Issue 3, pp. 557-62, 2009 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, 2007 (PubMed). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ding, Prieto, Zhang, Rosenthal, Smith, Skelton, Diwan: "Nuclear expression of the antiapoptotic protein survivin in malignant melanoma." in: Cancer, Vol. 106, Issue 5, pp. 1123-9, 2006 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Giodini, Kallio, Wall, Gorbsky, Tognin, Marchisio, Symons, Altieri: "Regulation of microtubule stability and mitotic progression by survivin." in: Cancer research, Vol. 62, Issue 9, pp. 2462-7, 2002 (PubMed).

Fortugno, Wall, Giodini, OConnor, Plescia, Padgett, Tognin, Marchisio, Altieri: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,ELISA,Immunofluorescence

Adida, Haioun, Gaulard, Lepage, Morel, Briere, Dombret, Reyes, Diebold, Gisselbrecht, Salles, Altieri, Molina: "Prognostic significance of survivin expression in diffuse large B-cell lymphomas." in: Blood, Vol. 96, Issue 5, pp. 1921-5, 2000 (PubMed).

Grossman, McNiff, Li, Altieri: "Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma." in: The Journal of investigative dermatology, Vol. 113, Issue 6, pp. 1076-81, 2000 (PubMed).

Li, Ackermann, Bennett, Rothermel, Plescia, Tognin, Villa, Marchisio, Altieri: "Pleiotropic cell-division defects and apoptosis induced by interference with survivin function." in: Nature cell biology, Vol. 1, Issue 8, pp. 461-6, 2000 (PubMed).

Background publications Li, Ambrosini, Chu, Plescia, Tognin, Marchisio, Altieri: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999 (PubMed).

Ambrosini, Adida, Sirugo, Altieri: "Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 11177-82, 1998 (PubMed).