Survivin antibody (BIRC5) (Cytoplasmic Form)

Details for Product anti-BIRC5 Antibody No. ABIN153038, Supplier: Log in to see
Antigen
  • bir5a
  • survivin
  • LOC398389
  • BIRC5
  • BIR1
  • Su2
  • Survivin
  • Survivin1
  • XBIR1
  • api4
  • epr-1
  • svv1
  • xSurvivin1
  • xsurv
  • SIX
  • Su1
  • svv2
  • birc5
  • xSurvivin
  • xSurvivin2
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • baculoviral IAP repeat-containing 5a
  • survivin
  • baculoviral IAP repeat-containing 5
  • baculoviral IAP repeat-containing 5, gene 1
  • baculoviral IAP repeat containing 5
  • birc5a
  • LOC398389
  • BIRC5
  • birc5.1
  • birc5.2
  • AaeL_AAEL012446
  • CpipJ_CPIJ001663
  • CpipJ_CPIJ002689
  • CpipJ_CPIJ006694
  • Birc5
Alternatives
anti-Cat (Feline) Survivin antibody for Chromatin Immunoprecipitation
Epitope
Cytoplasmic Form
65
30
26
18
18
16
15
15
12
7
7
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
438
167
163
35
20
19
12
4
2
2
1
Host
Mouse
300
145
16
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Survivin antibody is un-conjugated
24
17
16
13
13
13
13
12
10
10
8
8
6
5
5
5
5
4
4
4
2
2
2
2
2
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
349
206
182
158
147
126
113
33
32
26
8
5
3
3
3
3
2
2
2
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Clone 60-11
Isotype IgG2a kappa
Specificity This antibody is specific for the cytoplasmic form of Survivin.
Purification Protein G purified
Alternative Name Survivin (BIRC5 Antibody Abstract)
Background Gene Symbol: BIRC5
Molecular Weight Theoretical MW: 16.5 kDa
Gene ID 332
UniProt O15392
Research Area Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Pathways Nuclear Hormone Receptor Binding
Application Notes Western Blot 1:1000, Flow Cytometry 1 μg/mL, ELISA, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-FrozenThis Survivin (60.11) antibody is useful for Immunocytochemistry (PMID: 21976248)/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. Flow Cytometry and ELISA were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Survivin Antibody Immunohistochemistry - FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 0.9 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Staing of ovary, cancer using ABIN153038.
Western Blotting (WB) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Detection of Survivin in HeLa whole cell extract using ABIN153038.
Western Blotting (WB) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Western Blot: Survivin Antibody (60.11) [ABIN153038] - Detected in rat aorta smooth m...
 image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Flow (Intracellular): Survivin Antibody (60.11) [ABIN153038] - An intracellular stain...
Flow Cytometry (FACS) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Flow Cytometry: Survivin Antibody (60.11) [ABIN153038] - Staining of NTERA-2 cells us...
Western Blotting (WB) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Western Blot: Survivin Antibody (60.11) [ABIN153038] - Cells were transfected with th...
Immunofluorescence (IF) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [ABIN153038] - Dete...
Immunofluorescence (IF) image for anti-Survivin (BIRC5) (Cytoplasmic Form) antibody (ABIN153038) Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [ABIN153038] - IHeL...
Product cited in: Bae, Kim, Kim, Cheong, Ryu, Han: "Correlation of survivin and B-cell lymphoma 2 expression with pathological malignancy and anti-apoptotic properties of glial cell tumors." in: Biomedical reports, Vol. 6, Issue 4, pp. 396-400, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Dheekollu, Malecka, Wiedmer, Delecluse, Chiang, Altieri, Messick, Lieberman: "Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency." in: Oncotarget, Vol. 8, Issue 5, pp. 7248-7264, 2017 (PubMed). Method employed by authors: Proximity Ligation Assay (PLA)

Asuncion Valenzuela, Castro, Gonda, Diaz Osterman, Jutzy, Aspe, Khan, Neidigh, Wall: "Cell death in response to antimetabolites directed at ribonucleotide reductase and thymidylate synthase." in: OncoTargets and therapy, Vol. 8, pp. 495-507, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Kusner, Ciesielski, Marx, Kaminski, Fenstermaker: "Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study." in: PLoS ONE, Vol. 9, Issue 7, pp. e102231, 2014 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Kapinas, Kim, Mandeville, Martin-Buley, Croce, Lian, van Wijnen, Stein, Altieri, Stein: "microRNA-mediated survivin control of pluripotency." in: Journal of cellular physiology, Vol. 230, Issue 1, pp. 63-70, 2014 (PubMed). (Sample species: Human).

Rho, Lampe: "High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays." in: Journal of proteome research, Vol. 12, Issue 5, pp. 2311-20, 2013 (PubMed). (Sample species: Human).

Femia, Salvianti, Luceri, Dolara, Salvadori, Pinzani, Caderni: "Sustained proliferation and resistance to apoptosis after a cytotoxic insult are early alterations in rat colon carcinogenesis." in: International journal of cancer, Vol. 131, Issue 3, pp. 529-36, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Sakao, Desineni, Hahm, Singh: "Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo." in: The Prostate, Vol. 72, Issue 10, pp. 1104-16, 2012 (PubMed). Further details: Western Blotting,Immunohistochemistry

Keerthivasan, Liu, Gump, Dowdy, Wickrema, Crispino: "A novel role for survivin in erythroblast enucleation." in: Haematologica, Vol. 97, Issue 10, pp. 1471-9, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhang, Wang, Liu, Zhang, Xia, Zhang, Wu, Jiang, Chen, Wu, Zha, Yu, Kong: "DNA and adenovirus tumor vaccine expressing truncated survivin generates specific immune responses and anti-tumor effects in a murine melanoma model." in: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 10, pp. 1857-67, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Khan, Jutzy, Valenzuela, Turay, Aspe, Ashok, Mirshahidi, Mercola, Lilly, Wall: "Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer." in: PLoS ONE, Vol. 7, Issue 10, pp. e46737, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Lopergolo, Tavecchio, Lisanti, Ghosh, Dohi, Faversani, Vaira, Bosari, Tanigawa, Delia, Kossenkov, Showe, Altieri: "Chk2 phosphorylation of survivin-DeltaEx3 contributes to a DNA damage-sensing checkpoint in cancer." in: Cancer research, Vol. 72, Issue 13, pp. 3251-9, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

de Souza, West, Rebert, de la Motte, Drazba, Fiocchi: "Increased levels of survivin, via association with heat shock protein 90, in mucosal T cells from patients with Crohn's disease." in: Gastroenterology, Vol. 143, Issue 4, pp. 1017-26.e9, 2012 (PubMed). (Sample species: Human).

Lechler, Renkawitz, Campean, Balakrishnan, Tingart, Grifka, Schaumburger: "The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro." in: BMC cancer, Vol. 11, pp. 120, 2011 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Lechler, Schaumburger, Köck, Balakrishnan, Doukas, Prantl, Grifka: "The oncofetal gene survivin promotes cell proliferation and survival in primary human osteoblastic cells." in: Calcified tissue international, Vol. 89, Issue 3, pp. 211-20, 2011 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Lechler, Balakrishnan, Schaumburger, Grässel, Baier, Grifka, Straub, Renkawitz: "The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro." in: BMC musculoskeletal disorders, Vol. 12, pp. 150, 2011 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Knauer, Heinrich, Bier, Habtemichael, Docter, Helling, Mann, Stauber: "An otoprotective role for the apoptosis inhibitor protein survivin." in: Cell death & disease, Vol. 1, pp. e51, 2011 (PubMed).

Habtemichael, Heinrich, Knauer, Schmidtmann, Bier, Docter, Brochhausen, Helling, Brieger, Stauber, Mann: "Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear." in: Molecular and cellular neurosciences, Vol. 45, Issue 3, pp. 297-305, 2010 (PubMed).

Habtemichael, Wünsch, Bier, Tillmann, Unruhe, Frauenknecht, Heinrich, Mann, Stauber, Knauer: "Cloning and functional characterization of the guinea pig apoptosis inhibitor protein Survivin." in: Gene, Vol. 469, Issue 1-2, pp. 9-17, 2010 (PubMed).

Brocardo, Lei, Tighe, Taylor, Mok, Henderson: "Mitochondrial targeting of adenomatous polyposis coli protein is stimulated by truncating cancer mutations: regulation of Bcl-2 and implications for cell survival." in: The Journal of biological chemistry, Vol. 283, Issue 9, pp. 5950-9, 2008 (PubMed).

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