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Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody

Details for Product No. ABIN153048
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Antigen
Synonyms beclin-1, atg6, vps30, beclin1, Beclin-1, becn1, zgc:73097, ATG6, VPS30, 4921513J16Rik, 5430417M23Rik, Atg6
Epitope
AA 1-100
(24), (13), (9), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(211), (86), (59), (32), (30), (21), (18), (15), (12), (6), (2), (1), (1)
Host
Rabbit
(162), (56), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (9), (9), (9), (8), (8), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(185), (85), (69), (52), (38), (31), (17), (16), (15), (10), (5), (1)
Pubmed 9 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN153048
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Immunogen Internal synthetic peptide to human Beclin 1, within residues 1-100
Purification affinity purified
Alternative Name Beclin-1
Background Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumorsuppressor and antiviral function. Autophagy, a process of bulk protein degradationthrough an autophagosomic-lysosomal pathway, is important for differentiation, survivalduring nutrient deprivation, and normal growth control, and is often defective in tumorcells. Beclin 1 was originally isolated in a yeast two hybrid screen to identifyBcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome17q21 that is frequently monoallelically deleted in human breast, ovarian and prostatecancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that isexpressed in human muscle, epithelial cells and neurons. In gene transfer studies,beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammarytumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein isfrequently decreased in malignant breast epithelial cells. Based upon theseobservations, it is speculated that beclin 1 may work through induction of autophagy tonegatively regulate tumorigenesis and to control viral infections. Beclin 1 may also playa role in other biological processes in which autophagy is important such as celldifferentiation and nutritional stress responses. Alternate Names: anti-CTIP-1 COUP-TF interacting protein 1 antibody, anti-Beclin1 autophagy antibody,anti-Coiled-coil myosin-like BCL2-interacting protein antibody, anti-Beclin-1 antibody,anti-Protein GT197 antibody, anti-APG6 antibody.
Gene Symbol: BECN1
Gene ID 8678, 56208
UniProt Q14457
Application Notes This Beclin 1 Antibody is useful for Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry paraffin-embedded sections. In Western Blot, a band is seen at ~52 kDa representing Beclin 1.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:400, Immunohistochemistry-Paraffin 1:400, immunoprecipitation 1:80, Western Blot 1:500-1:2000
Protocol Immunoprecipitation protocol specific for Beclin 1 Antibody Immunoprecipitation Protocol:
1. Cells in 2x 75cm flasks (60 % confluency) are scraped with 0.5ml of Tris lysis
Restrictions For Research Use only
Format Liquid
Concentration 1.15 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody Detection of Beclin in mouse liver tissue lysate (50 ug) using ABIN153048. ECL detection at 1 minute.
anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (2) Pheochromocytes of the Adrenal Medulla 40x
General Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Massey, Kaushik, Sovak et al.: "Consequences of the selective blockage of chaperone-mediated autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5805-10, 2006 (PubMed).

Park, Lee, Kim et al.: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).

Wang, Singh, Massey et al.: "Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4766-77, 2008 (PubMed).

Massey, Follenzi, Kiffin et al.: "Early cellular changes after blockage of chaperone-mediated autophagy." in: Autophagy, Vol. 4, Issue 4, pp. 442-56, 2008 (PubMed).

Gulati, Gaspers, Dann et al.: "Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34." in: Cell metabolism, Vol. 7, Issue 5, pp. 456-65, 2008 (PubMed).

Pattingre, Bauvy, Carpentier et al.: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009 (PubMed).

Validation Images
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