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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Beclin 1, Autophagy Related (BECN1) antibody
|Synonyms||ATG6, VPS30, beclin1, MGC6843, 4921513J16Rik, 5430417M23Rik, MGC73097, zgc:73097, BECN1, becn1, MGC80672, MGC126978, atg6, vps30, Beclin-1, DKFZp459C0719|
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|9 references available|
|Quantity||0.1 ml (1 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Gene ID||8678, 56208|
|Immunogen||ABIN153048 was raised against an internal synthetic peptide to human Beclin 1,containing residues within 1-100. This sequence has 94% identity with the mousesequence and 88% identity with the rat sequence.|
|Description||Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumorsuppressor and antiviral function. Autophagy, a process of bulk protein degradationthrough an autophagosomic-lysosomal pathway, is important for differentiation, survivalduring nutrient deprivation, and normal growth control, and is often defective in tumorcells. Beclin 1 was originally isolated in a yeast two hybrid screen to identifyBcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome17q21 that is frequently monoallelically deleted in human breast, ovarian and prostatecancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that isexpressed in human muscle, epithelial cells and neurons. In gene transfer studies,beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammarytumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein isfrequently decreased in malignant breast epithelial cells. Based upon theseobservations, it is speculated that beclin 1 may work through induction of autophagy tonegatively regulate tumorigenesis and to control viral infections. Beclin 1 may also playa role in other biological processes in which autophagy is important such as celldifferentiation and nutritional stress responses. Alternate Names: anti-CTIP-1 COUP-TF interacting protein 1 antibody, anti-Beclin1 autophagy antibody,anti-Coiled-coil myosin-like BCL2-interacting protein antibody, anti-Beclin-1 antibody,anti-Protein GT197 antibody, anti-APG6 antibody.|
|Specificity||This antibody is specific to human Beclin 1. Species Reactivity: This antibody can be used for detection of Beclin 1 in human samples. Preliminaryresults suggest that this antibody may cross-react with mouse protein, as well. Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cellbodies of cerebellar Purkinje cells.|
|Application Notes||Suggested working dilutions: Western Blot 1:2000 - 1:5000|
|Purification||Aff - Purified|
|Buffer||Tris-citrate/phosphate, pH 7-8. Preservative: 0.1% sodium azide.|
|Storage||Store at 4C. Do not freeze.|
|Restrictions||For Research Use only|
|Detection of Beclin in mouse liver tissue lysate (50 ug) using ABIN153048. ECL detection at 1 minute. Pheochromocytes of the Adrenal Medulla 40x|
Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).
Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).
Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).
Massey, Kaushik, Sovak et al.: "Consequences of the selective blockage of chaperone-mediated autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5805-10, 2006 (PubMed).
Park, Lee, Kim et al.: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).
Wang, Singh, Massey et al.: "Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4766-77, 2008 (PubMed).
Massey, Follenzi, Kiffin et al.: "Early cellular changes after blockage of chaperone-mediated autophagy." in: Autophagy, Vol. 4, Issue 4, pp. 442-56, 2008 (PubMed).
Gulati, Gaspers, Dann et al.: "Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34." in: Cell metabolism, Vol. 7, Issue 5, pp. 456-65, 2008 (PubMed).
Pattingre, Bauvy, Carpentier et al.: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009 (PubMed).
|Hosts||Rabbit (75), Mouse (17), Sheep (2)|
|Reactivities||Human (90), Mouse (Murine) (42), Rat (Rattus) (35), Dog (Canine) (22), Cow (Bovine) (19), Horse (Equine) (18), Pig (Porcine) (18), Monkey (3), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (78), ELISA (37), Immunofluorescence (IF) (34), Immunohistochemistry (IHC) (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Flow Cytometry (FACS) (11), Immunocytochemistry (ICC) (9), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (2), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (10), C-Term (7), Internal Region (2), AA 53-72 (1), N-Term,Thr72 (1)|