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Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100)

Details for Product anti-BECN1 Antibody No. ABIN153048, Supplier: Log in to see
Antigen
  • 4921513J16Rik
  • 5430417M23Rik
  • atg6
  • ATG6
  • Atg6
  • beclin-1
  • Beclin-1
  • beclin1
  • becn1
  • VPS30
  • vps30
  • zgc:73097
Epitope
AA 1-100
34
33
19
16
14
14
13
13
13
12
11
11
9
8
8
8
6
6
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
408
128
125
27
15
14
13
13
13
8
6
3
1
1
1
1
Host
Rabbit
318
95
3
Clonality
Polyclonal
Conjugate
This Beclin 1 antibody is un-conjugated
22
21
21
19
19
19
7
7
7
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
333
168
160
94
67
59
31
30
27
12
12
6
3
2
1
Supplier
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Immunogen Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457].
Purification Immunogen affinity purified
Alternative Name Beclin 1 (BECN1 Antibody Abstract)
Background Gene Symbol: BECN1
Gene ID 8678, 56208
UniProt Q14457
Research Area Transcription Factors, Autophagy, Cancer
Application Notes Western Blot 1:500-1:2000, Simple Western 1:50, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:80, Immunohistochemistry-Paraffin 1:400
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunoprecipitation protocol specific for Beclin 1 Antibody Immunoprecipitation Protocol:
1. Cells in 2x 75cm flasks (60 % confluency) are scraped with 0.5ml of Tris lysis
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS pH 7.4
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Detection of Beclin in mouse liver tissue lysate (50 ug) using ABIN153048. ECL detec...
Immunohistochemistry (IHC) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Pheochromocytes of the Adrenal Medulla 40x
Immunohistochemistry (IHC) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Immunohistochemistry: Beclin 1/ATG6 Antibody [ABIN153048] - ATG6 on pheochromocytes o...
Simple Western (SimWes) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Simple Western: Beclin 1/ATG6 Antibody [ABIN153048] - Simple Western lane view shows ...
Western Blotting (WB) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Western Blot: Beclin 1/ATG6 Antibody [ABIN153048] - WB analysis of Belclin1. Lane 1 h...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Immunohistochemistry-Paraffin: Beclin 1/ATG6 Antibody [ABIN153048] - Analysis of Becl...
Immunofluorescence (IF) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100) (ABIN153048) Immunocytochemistry/Immunofluorescence: Beclin 1/ATG6 Antibody [ABIN153048] - Beclin ...
Product cited in: Zhang, Ding, Wang, Wu, Xu: "Traumatic Brain Injury-Induced Neuronal Apoptosis is Reduced Through Modulation of PI3K and Autophagy Pathways in Mouse by FTY720." in: Cellular and molecular neurobiology, Vol. 36, Issue 1, pp. 131-42, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Masuda, Yashiro, Kitayama, Miki, Kasashima, Kinoshita, Morisaki, Fukuoka, Hasegawa, Sakurai, Toyokawa, Kubo, Tanaka, Muguruma, Masaichi, Hirakawa: "Clinicopathological Correlations of Autophagy-related Proteins LC3, Beclin 1 and p62 in Gastric Cancer." in: Anticancer research, Vol. 36, Issue 1, pp. 129-36, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ueno, Saji, Sugimoto, Masuda, Kuroi, Sato, Takei, Yamamoto, Ohno, Yamashita, Hisamatsu, Aogi, Iwata, Imoto, Sasano, Toi: "Clinical significance of the expression of autophagy-associated marker, beclin 1, in breast cancer patients who received neoadjuvant endocrine therapy." in: BMC cancer, Vol. 16, pp. 230, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Choudhury, Bucha, Baksi, Mukhopadhyay, Bhattacharyya: "Chaperone-like protein HYPK and its interacting partners augment autophagy." in: European journal of cell biology, Vol. 95, Issue 6-7, pp. 182-94, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kivinen, Felszeghy, Kinnunen, Setälä, Aikio, Kinnunen, Sironen, Pihlajaniemi, Kauppinen, Kaarniranta: "Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis." in: Biogerontology, Vol. 17, Issue 4, pp. 749-61, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Hong, Kim, Lee: "Role of necroptosis in autophagy signaling during hepatic ischemia and reperfusion." in: Toxicology and applied pharmacology, Vol. 308, pp. 1-10, 2016 (PubMed). Further details: Western Blotting

Cheong, Kim, Eom, Jeung, Min: "Enhanced autophagy in cytarabine arabinoside-resistant U937 leukemia cells and its potential as a target for overcoming resistance." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3433-40, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Wang, Pei, Chen, Zhang: "Increased Apoptosis of Inflamed Odontoblasts Is Associated with CD47 Loss." in: Journal of dental research, Vol. 95, Issue 6, pp. 697-703, 2016 (PubMed).

Jang, Eom, Jeung, Cheong, Lee, Kim, Min: "AMPK-ULK1-mediated autophagy confers resistance to BET inhibitor JQ1 in acute myeloid leukemia stem cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Hu, Zou, Huang, Zhang, Lourenco, Chen, Halliday, Wang, Ren: "ROCK1 Is Associated with Alzheimer's Disease-Specific Plaques, as well as Enhances Autophagosome Formation But not Autophagic Aβ Clearance." in: Frontiers in cellular neuroscience, Vol. 10, pp. 253, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Wakabayashi, Mori, Kakita, Takahashi, Utsumi, Sasaki: "Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis." in: Acta neuropathologica communications, Vol. 2, pp. 173, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Casalino-Matsuda, Nair, Beitel, Gates, Sporn: "Hypercapnia Inhibits Autophagy and Bacterial Killing in Human Macrophages by Increasing Expression of Bcl-2 and Bcl-xL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 11, pp. 5388-96, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhang, Wang, Ding, Xu: "FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells." in: Toxicology letters, Vol. 236, Issue 1, pp. 43-59, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Dever, Rodriguez, Lapierre, Costin, El-Hage: "Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure." in: Frontiers in microbiology, Vol. 6, pp. 653, 2015 (PubMed). Further details: Western Blotting

Siddiqui, Mukherjee, Manivannan, Malathi: "RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 17611-36, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Tanji, Miki, Maruyama, Mimura, Matsumiya, Mori, Imaizumi, Itoh, Wakabayashi: "Trehalose intake induces chaperone molecules along with autophagy in a mouse model of Lewy body disease." in: Biochemical and biophysical research communications, Vol. 465, Issue 4, pp. 746-52, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Liu, Li, Lu, Zong, Luo, Sun, Guo: "Baclofen mediates neuroprotection on hippocampal CA1 pyramidal cells through the regulation of autophagy under chronic cerebral hypoperfusion." in: Scientific reports, Vol. 5, pp. 14474, 2015 (PubMed). Further details: Western Blotting

Majumder, Chakrabarti: "Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101." in: Cell death & disease, Vol. 6, pp. e1970, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Park, Park, Ko, Shin, Koh: "mTOR inhibition by rapamycin protects against deltamethrin-induced apoptosis in PC12 Cells." in: Environmental toxicology, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Laguna, Schintu, Nobre, Alvarsson, Volakakis, Jacobsen, Gómez-Galán, Sopova, Joodmardi, Yoshitake, Deng, Kehr, Ericson, Svenningsson, Shupliakov, Perlmann: "Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease." in: Nature neuroscience, Vol. 18, Issue 6, pp. 826-35, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting