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NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (C-Term)

Details for Product anti-NOD2 Antibody No. ABIN153051, Supplier: Log in to see
Antigen
  • ACUG
  • BLAU
  • CARD15
  • CD
  • CLR16.3
  • IBD1
  • NLRC2
  • NOD2B
  • PSORAS1
  • Card15
  • F830032C23Rik
  • Nlrc2
  • caspase recruitment domain 15 protein
  • nucleotide-binding oligomerization domain containing 2
  • TTHERM_01951320
  • NOD2
  • Nod2
Alternatives
anti-Human NOD2 antibody for Flow Cytometry
Epitope
C-Term, AA 1000-1040
35
25
12
12
11
5
4
3
1
1
1
Reactivity
Human
145
52
20
1
1
Host
Rabbit
95
33
17
6
Clonality
Polyclonal
Conjugate
This NOD2 antibody is un-conjugated
6
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
126
47
46
42
37
35
30
23
13
5
4
2
2
Options
Supplier
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Immunogen A synthetic peptide made to the C-terminal region of human NOD2 (between residues 1000-1040). [UniProt# Q9HC29]
Purification Immunogen affinity purified
Alternative Name NOD2 (NOD2 Antibody Abstract)
Background Gene Symbol: NOD2
Molecular Weight Theoretical MW: 110 kDa
Gene ID 64127
UniProt Q9HC29
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Application Notes Western Blot 0.5-2 μg/mL, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50 - 1:100This NOD2 antibody has been tested for Western blot on NOD2 transfected 293T lysates where a band is seen at ~110 kDa. We did not see positive results probing endogenous protein using HT29 lysates. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for NOD2 Antibody
Western Blot Protocol:
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 20 µg of total transfected protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1 hour.
. Dilute the rabbit anti-NOD2 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.87 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (C-Term) (ABIN153051) Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using ABIN153051. ECL...
Immunofluorescence (IF) image for anti-NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (C-Term) (ABIN153051) Immunocytochemistry/Immunofluorescence: NOD2 Antibody [ABIN153051] - NOD2 antibody wa...
Product cited in: Lappas: "NOD1 and NOD2 regulate proinflammatory and prolabor mediators in human fetal membranes and myometrium via nuclear factor-kappa B." in: Biology of reproduction, Vol. 89, Issue 1, pp. 14, 2013 (PubMed).

Lipinski, Grabe, Jacobs, Billmann-Born, Till, Häsler, Aden, Paulsen, Arlt, Kraemer, Hagemann, Erdmann, Schreiber, Rosenstiel: "RNAi screening identifies mediators of NOD2 signaling: implications for spatial specificity of MDP recognition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 52, pp. 21426-31, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence,Immunohistochemistry

Till, Rosenstiel, Bräutigam, Sina, Jacobs, Oberg, Seegert, Chakraborty, Schreiber: "A role for membrane-bound CD147 in NOD2-mediated recognition of bacterial cytoinvasion." in: Journal of cell science, Vol. 121, Issue Pt 4, pp. 487-95, 2008 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Abbott, Wilkins, Asara, Cantley: "The Crohn's disease protein, NOD2, requires RIP2 in order to induce ubiquitinylation of a novel site on NEMO." in: Current biology : CB, Vol. 14, Issue 24, pp. 2217-27, 2004 (PubMed).

Hisamatsu, Suzuki, Reinecker, Nadeau, McCormick, Podolsky: "CARD15/NOD2 functions as an antibacterial factor in human intestinal epithelial cells." in: Gastroenterology, Vol. 124, Issue 4, pp. 993-1000, 2003 (PubMed).