Proteasome 20S C2 antibody

Details for Product No. ABIN153064, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast
16
16
16
Host
Rabbit
16
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
13
4
4
1
1
1
Options
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Immunogen A proteasomal preparation isolated from human red blood cells.
Specificity This detects a combination of alpha and beta proteasome subunits.
Purification Unpurified
Background Gene Symbol: PSMA1
Gene ID 5682
UniProt P25786
Research Area Proteolysis / Ubiquitin, Proteasome
Application Notes Western Blot 1:1000-1:10000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500Western blotting - The antibody has been characterized by single dimension SDS-PAGE using both purified human and yeast 20S proteasome and a number of tissue/cell preparations including a human placental proteasome preparation, a HeLa cell lysate, a RSV 3T3 mouse fibroblast cell lysate, and a yeast whole cell extract.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer Whole antisera with PBS
Buffer contains: 10 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Proteasome 20S C2 antibody (ABIN153064) Luminograph of human erythrocyte-derived 20S proteasome lysate after SDS-PAGE followe...
Product cited in: Liu, Tsai, Morleo, Oh, Leitch, Massa, Lee, Parker, Finley, Zaghloul, Franco, Katsanis: "Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators." in: The Journal of clinical investigation, Vol. 124, Issue 5, pp. 2059-70, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Laporte, Salin, Daignan-Fornier, Sagot: "Reversible cytoplasmic localization of the proteasome in quiescent yeast cells." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 737-45, 2008 (PubMed).

Funakoshi, Sasaki, Nishimoto, Kobayashi: "Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 745-50, 2002 (PubMed).

Lelouard, Gatti, Cappello, Gresser, Camosseto, Pierre: "Transient aggregation of ubiquitinated proteins during dendritic cell maturation." in: Nature, Vol. 417, Issue 6885, pp. 177-82, 2002 (PubMed).

Xie, Varshavsky: "UFD4 lacking the proteasome-binding region catalyses ubiquitination but is impaired in proteolysis." in: Nature cell biology, Vol. 4, Issue 12, pp. 1003-7, 2002 (PubMed).

Kopp, Hendil, Dahlmann, Kristensen, Sobek, Uerkvitz: "Subunit arrangement in the human 20S proteasome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 7, pp. 2939-44, 1997 (PubMed).

Tanaka, Tsurumi: "The 26S proteasome: subunits and functions." in: Molecular biology reports, Vol. 24, Issue 1-2, pp. 3-11, 1997 (PubMed).