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Coatomer Protein Complex, Subunit beta 1 (COPB1) antibody

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Synonyms id:ibd5034, wu:fc66c05, zgc:92482, COPB1, DKFZp459K154, COPB, 2610019B04Rik, Rack2, Beta-COP
Bird (Avian), Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Rat (Rattus)
(31), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1)
(26), (7)
Clonality (Clone)
Monoclonal ()
(2), (2), (2), (2), (2), (2)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(25), (22), (4), (3), (3), (2), (1), (1)
Pubmed 10 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen Microtubule-associated protein from goose brain.
Clone M3A5
Isotype IgG1
Specificity Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).
Cross-Reactivity (Details) Not yet tested in other species.
Purification Ascites
Alternative Name Coatomer subunit beta (COPB) (COPB1 Antibody Abstract)
Background May be used for studies on the effects of various agents influencing energy status, ondisrupting the Golgi complex, or on altering the activity of G-proteins or smallGTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinitypurification of the beta-COP protein. Alternate Names: Anti-Beta-coat protein antibody, Anti-betacop antibody, Anti-Coatomer beta subunitantibody, Anti-Coatomer protein complex subunit beta 1 antibody, Anti-Coatomer proteincomplex subunit beta antibody, Anti-Coatomer subunit beta antibody, Anti-COPBantibody, Anti-COPB1 antibody, Anti-DKFZp761K102 antibody, Anti-FLJ10341 antibody.
Gene Symbol: COPB2
Gene ID 9276, 60384
Application Notes AF: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: CHO cells IP: Use at an assay dependant dilution. Stacked Golgi membranes from rat liver extract. Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.
Recommended dilutions: Immunoprecipitation 1:10-1:500, Western Blot 1:50-1:1000, Immunocytochemistry/Immunofluorescence 1:20-1:50
Restrictions For Research Use only
Format Liquid
Buffer ascites fluid, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Product cited in: McGugan, Temesvari: "Characterization of a Rab11-like GTPase, EhRab11, of Entamoeba histolytica." in: Molecular and biochemical parasitology, Vol. 129, Issue 2, pp. 137-46, 2003 (PubMed).

Welter, Laughlin, Temesvari: "Characterization of a Rab7-like GTPase, EhRab7: a marker for the early stages of endocytosis in Entamoeba histolytica." in: Molecular and biochemical parasitology, Vol. 121, Issue 2, pp. 254-64, 2002 (PubMed).

Background publications Orci, Palmer, Ravazzola et al.: "Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins." in: Nature, Vol. 362, Issue 6421, pp. 648-52, 1993 (PubMed).

Allan, Kreis: "A microtubule-binding protein associated with membranes of the Golgi apparatus." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2229-39, 1987 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Duden, Ho, Allan et al.: "What's new in cytoskeleton-organelle interactions? Relationship between microtubules and the Golgi-apparatus." in: Pathology, research and practice, Vol. 186, Issue 4, pp. 535-41, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Klausner: "Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus." in: The Journal of cell biology, Vol. 112, Issue 4, pp. 579-88, 1991 (PubMed).

Donaldson, Kahn, Lippincott-Schwartz et al.: "Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein." in: Science (New York, N.Y.), Vol. 254, Issue 5035, pp. 1197-9, 1991 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Sugai, Chen, Wu: "Bacterial ADP-ribosyltransferase with a substrate specificity of the rho protein disassembles the Golgi apparatus in Vero cells and mimics the action of brefeldin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 8903-7, 1992 (PubMed).

Catalog No. ABIN153068
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