Coatomer Protein Complex, Subunit beta 1 (COPB1) antibody

Antigen: Coatomer Protein Complex, Subunit beta 1 (COPB1)

Synonyms: COPB, FLJ10341, FLJ46444, FLJ57957, DKFZp761K102, beta'-COP, 2610019B04Rik, Rack2, MGC92482, zgc:92482, id:ibd5034, wu:fc66c05, MGC80934, COPB1, MGC139703, DKFZp459K154

Clonality: Monoclonal (M3A5)

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN153068

Additional Information

Alternative name: Coatomer subunit beta (COPB)

Gene ID: 9276, 60384

Immunogen: Full length native protein (purified). (Goose).

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Hamster, Human, Rat (Rattus)

Format: Ascites

Isotype: IgG1

Clone: M3A5

Description: May be used for studies on the effects of various agents influencing energy status, ondisrupting the Golgi complex, or on altering the activity of G-proteins or smallGTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinitypurification of the beta-COP protein. Alternate Names: Anti-Beta-coat protein antibody, Anti-betacop antibody, Anti-Coatomer beta subunitantibody, Anti-Coatomer protein complex subunit beta 1 antibody, Anti-Coatomer proteincomplex subunit beta antibody, Anti-Coatomer subunit beta antibody, Anti-COPBantibody, Anti-COPB1 antibody, Anti-DKFZp761K102 antibody, Anti-FLJ10341 antibody.

Specificity: Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissueculture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2,270-300 kD). Species Reactivity: Cross-reacts with Chicken, Cow, Dog, Goose, Hamster, Human, Monkey, Rat kangarooand Rat.Not yet tested in other species. Localization: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, aswell as on the vesicles/buds originating from it.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1/20 - 1/50,Western Blot 1/50 - 1/1000,AF

Concentration: 0.3 mg/ml

Purification: Ascites

Buffer: Preservative: 0.1% Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Publications

Sugai, Chen, Wu: "Bacterial ADP-ribosyltransferase with a substrate specificity of the rho protein disassembles the Golgi apparatus in Vero cells and mimics the action of brefeldin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 8903-7, 1992 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Donaldson, Kahn, Lippincott-Schwartz et al.: "Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein." in: Science (New York, N.Y.), Vol. 254, Issue 5035, pp. 1197-9, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Klausner: "Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus." in: The Journal of cell biology, Vol. 112, Issue 4, pp. 579-88, 1991 (PubMed).

Duden, Ho, Allan et al.: "What's new in cytoskeleton-organelle interactions? Relationship between microtubules and the Golgi-apparatus." in: Pathology, research and practice, Vol. 186, Issue 4, pp. 535-41, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom et al.: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Allan, Kreis: "A microtubule-binding protein associated with membranes of the Golgi apparatus." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2229-39, 1987 (PubMed).

Orci, Palmer, Ravazzola et al.: "Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins." in: Nature, Vol. 362, Issue 6421, pp. 648-52, 1993 (PubMed).

Welter, Laughlin, Temesvari: "Characterization of a Rab7-like GTPase, EhRab7: a marker for the early stages of endocytosis in Entamoeba histolytica." in: Molecular and biochemical parasitology, Vol. 121, Issue 2, pp. 254-64, 2002 (PubMed).

McGugan, Temesvari: "Characterization of a Rab11-like GTPase, EhRab11, of Entamoeba histolytica." in: Molecular and biochemical parasitology, Vol. 129, Issue 2, pp. 137-46, 2003 (PubMed).