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Coatomer Protein Complex, Subunit beta 2 (COPB2) antibody

Details for Product No. ABIN153068, Supplier: Log in to see
Antigen
  • sb:cb121
  • wu:fb30f06
  • COPB
  • Beta-COP
  • 2610019B04Rik
  • beta'-COP
  • AI256832
  • coatomer protein complex, subunit beta 2
  • coatomer beta' subunit
  • coatomer beta subunit
  • Coatomer beta' subunit
  • beta-cop
  • coatomer protein complex, subunit beta 1
  • coatomer protein complex, subunit beta 2 (beta prime)
  • copb2
  • NCU07319
  • ATEG_01512
  • beta-coP
  • LELG_04874
  • HCAG_08724
  • GL50803_9593
  • COPB1
  • CTRG_02922
  • UREG_02221
  • EBI_25283
  • Copb1
  • Copb2
  • COPB2
Reactivity
Bird (Avian), Chicken, Cow (Bovine), Dog (Canine), Hamster, Human, Monkey, Primate, Rabbit, Rat (Rattus)
28
17
7
1
1
1
1
1
1
Host
Mouse
28
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
25
15
5
5
3
2
2
1
1
Options
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Immunogen Microtubule-associated protein from goose brain.
Clone M3A5
Isotype IgG1
Specificity Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).
Purification Unpurified
Alternative Name COPB2 (COPB2 Antibody Abstract)
Background Gene Symbol: COPB2
Gene ID 9276
Application Notes Western Blot 1:50-1:1000, Immunocytochemistry/Immunofluorescence 1:20-1:50, Immunoprecipitation 1:10-1:500AF: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: CHO cells IP: Use at an assay dependant dilution. Stacked Golgi membranes from rat liver extract. Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Product cited in: Cianciola, Carlin: "Adenovirus RID-alpha activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C." in: The Journal of cell biology, Vol. 187, Issue 4, pp. 537-52, 2009 (PubMed).

McGugan, Temesvari: "Characterization of a Rab11-like GTPase, EhRab11, of Entamoeba histolytica." in: Molecular and biochemical parasitology, Vol. 129, Issue 2, pp. 137-46, 2003 (PubMed).

Welter, Laughlin, Temesvari: "Characterization of a Rab7-like GTPase, EhRab7: a marker for the early stages of endocytosis in Entamoeba histolytica." in: Molecular and biochemical parasitology, Vol. 121, Issue 2, pp. 254-64, 2002 (PubMed).

Donaldson, Lippincott-Schwartz, Klausner: "Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus." in: The Journal of cell biology, Vol. 112, Issue 4, pp. 579-88, 1991 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Christensen, Schweppe, Jungmann: "Cyclic AMP-dependent and -independent protein phosphokinase activity in subcellular fractions of synchronously growing leydig I-10 cells." in: Experimental cell research, Vol. 124, Issue 1, pp. 15-24, 1980 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Background publications Orci, Palmer, Ravazzola, Perrelet, Amherdt, Rothman: "Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins." in: Nature, Vol. 362, Issue 6421, pp. 648-52, 1993 (PubMed).

Sugai, Chen, Wu: "Bacterial ADP-ribosyltransferase with a substrate specificity of the rho protein disassembles the Golgi apparatus in Vero cells and mimics the action of brefeldin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 8903-7, 1992 (PubMed).

Ktistakis, Roth, Bloom: "PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1009-23, 1991 (PubMed).

Donaldson, Lippincott-Schwartz, Bloom, Kreis, Klausner: "Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2295-306, 1991 (PubMed).

Donaldson, Kahn, Lippincott-Schwartz, Klausner: "Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein." in: Science (New York, N.Y.), Vol. 254, Issue 5035, pp. 1197-9, 1991 (PubMed).

Duden, Ho, Allan, Kreis: "What's new in cytoskeleton-organelle interactions? Relationship between microtubules and the Golgi-apparatus." in: Pathology, research and practice, Vol. 186, Issue 4, pp. 535-41, 1991 (PubMed).

Allan, Kreis: "A microtubule-binding protein associated with membranes of the Golgi apparatus." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2229-39, 1987 (PubMed).