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CD28 antibody (pTyr218)

CD28 Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1531543
  • Target See all CD28 Antibodies
    CD28
    Binding Specificity
    • 16
    • 15
    • 10
    • 10
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 171-220, pTyr218
    Reactivity
    • 172
    • 127
    • 58
    • 38
    • 21
    • 20
    • 12
    • 12
    • 7
    • 7
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 126
    • 78
    • 33
    • 16
    • 8
    • 3
    • 2
    • 1
    Rabbit
    Clonality
    • 188
    • 73
    • 3
    Polyclonal
    Conjugate
    • 87
    • 30
    • 29
    • 21
    • 14
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD28 antibody is un-conjugated
    Application
    • 199
    • 93
    • 47
    • 47
    • 37
    • 31
    • 27
    • 26
    • 19
    • 17
    • 13
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificity

    CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.

    PhosphorylationH:Y218 M:Y216

    Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
    Purity
    > 95 %
    Immunogen
    The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
    Isotype
    IgG
    Top Product
    Discover our top product CD28 Primary Antibody
  • Application Notes
    WB: 1:500~1:1000 ELISA: 1:40000
    Comment

    Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Stable at -20°C for at least 1 year.
    Expiry Date
    12 months
  • Target
    CD28
    Alternative Name
    CD28 (CD28 Products)
    Synonyms
    Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
    Background

    Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen

    NCBI Gene Symbol: CD28

    Molecular Weight
    25 kDa
    Gene ID
    940
    OMIM
    186760
    UniProt
    P10747
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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